『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=186163)

EID=186163EID:186163, Map:0, LastModified:2012年9月27日(木) 17:01:16, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[佐田 政隆], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Meguro Kentaro (日) (読)
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学籍番号 (推奨):
[継承]
2. (英) Iida Haruko (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Takano Haruhito (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Morita Toshihiro (日) (読)
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学籍番号 (推奨):
[継承]
5.佐田 政隆 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.循環器内科学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Nagai Ryozo (日) (読)
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学籍番号 (推奨):
[継承]
7. (英) Nakajima Toshiaki (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Function and Role of Voltage-Gated Sodium Channel (NaV1.7) Expressed in Aortic Smooth Muscle Cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Voltage-gated Na(+) channel currents (I(Na)) are expressed in several types of smooth muscle cells. The purpose of this study was to evaluate the expression of I(Na), its functional role, pathophysiology in cultured human (hASMCs) and rabbit aortic smooth muscle cells (rASMCs), and its association with vascular intimal hyperplasia. In whole cell voltage clamp, I(Na) was observed at potential positive to -40 mV, was blocked by tetrodotoxin (TTX), and replacing extracellular Na(+) with N-methyl-d-glucamine in cultured hASMCs. In contrast to native aorta, cultured hASMCs strongly expressed SCN9A encoding Na(V)1.7, as determined by quantitative RT-PCR. I(Na) was abolished by the treatment with SCN9A small-interfering (si)RNA (P < 0.01). TTX and SCN9A siRNA significantly inhibited cell migration (P < 0.01, respectively) and horseradish peroxidase uptake (P < 0.01, respectively). TTX also significantly reduced the secretion of matrix metalloproteinase-2 6 and 12 h after the treatment (P < 0.01 and P < 0.05, respectively). However, neither TTX nor siRNA had any effect on cell proliferation. L-type Ca(2+) channel current was recorded, and I(Na) was not observed in freshly isolated rASMCs, whereas TTX-sensitive I(Na) was recorded in cultured rASMCs. Quantitative RT-PCR and immunostaining for Na(V)1.7 revealed the prominent expression of SCN9A in cultured rASMCs and aorta 48 h after balloon injury but not in native aorta. In conclusion, these studies show that I(Na) is expressed in cultured and diseased conditions but not in normal aorta. The Na(V)1.7 plays an important role in cell migration, endocytosis, and secretion. Na(V)1.7 is also expressed in aorta after balloon injury, suggesting a potential role for Na(V)1.7 in the progression of intimal hyperplasia.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Aorta, Thoracic (日) (読) [継承]
3. (英) Balloon Dilation (日) (読) [継承]
4. (英) Cell Movement (日) (読) [継承]
5. (英) Endocytosis (日) (読) [継承]
6. (英) Humans (日) (読) [継承]
7.免疫組織化学 (immunohistochemistry) [継承]
8. (英) Male (日) (読) [継承]
9. (英) Matrix Metalloproteinase 8 (日) (読) [継承]
10. (英) Myocytes, Smooth Muscle (日) (読) [継承]
11.RNA (RNA) [継承]
12. (英) Rabbits (日) (読) [継承]
13. (英) Rats (日) (読) [継承]
14. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
15. (英) Sodium Channel Blockers (日) (読) [継承]
16. (英) Sodium Channels (日) (読) [継承]
17. (英) Tetrodotoxin (日) (読) [継承]
発行所 (推奨):
誌名 (必須): American Journal of Physiology, Heart and Circulatory Physiology ([アメリカ生理学会])
(pISSN: 0363-6135, eISSN: 1522-1539)

ISSN (任意): 0363-6135
ISSN: 0363-6135 (pISSN: 0363-6135, eISSN: 1522-1539)
Title: American journal of physiology. Heart and circulatory physiology
Title(ISO): Am J Physiol Heart Circ Physiol
Publisher: American Physiological Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 296 [継承]
(必須): 1 [継承]
(必須): H211 219 [継承]
都市 (任意):
年月日 (必須): 西暦 2009年 1月 初日 (平成 21年 1月 初日) [継承]
URL (任意):
DOI (任意): 10.1152/ajpheart.00960.2008    (→Scopusで検索) [継承]
PMID (任意): 18978189    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Dept. of Cardiovascular Medicine, Univ. of Tokyo Graduate School of Medicine, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan. meguro@med.email.ne.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Kentaro Meguro, Haruko Iida, Haruhito Takano, Toshihiro Morita, Masataka Sata, Ryozo Nagai and Toshiaki Nakajima : Function and Role of Voltage-Gated Sodium Channel (NaV1.7) Expressed in Aortic Smooth Muscle Cells., American Journal of Physiology, Heart and Circulatory Physiology, Vol.296, No.1, H211-219, 2009.
欧文冊子 ● Kentaro Meguro, Haruko Iida, Haruhito Takano, Toshihiro Morita, Masataka Sata, Ryozo Nagai and Toshiaki Nakajima : Function and Role of Voltage-Gated Sodium Channel (NaV1.7) Expressed in Aortic Smooth Muscle Cells., American Journal of Physiology, Heart and Circulatory Physiology, Vol.296, No.1, H211-219, 2009.

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