『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
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学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.新田 剛
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2. (英) Igarashi Kazuei (日) (読)
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3. (英) Yamashita Atsuya (日) (読)
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4. (英) Yamamoto Mikio (日) (読)
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5. (英) Yamamoto Naoki (日) (読)
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題名 (必須): (英) Involvement of polyamines in B cell receptor-mediated apoptosis: spermine functions as a negative modulator.  (日)    [継承]
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要約 (任意): (英) The B cell lymphoma WEHI231 has been used as a model for studying clonal deletion of B cells on the basis of its ability to undergo growth arrest and apoptosis by B cell antigen receptor (BCR) cross-linking. To comprehensively analyze the genes involved in BCR-mediated apoptosis, we applied the technique of serial analysis of gene expression (SAGE) to WEHI231. Comparison of expression patterns revealed that BCR cross-linking caused coordinate changes in the expression of genes involved in polyamine metabolism. Polyamines are ubiquitous compounds required for cell proliferation and homeostasis. The coordinate expression of the polyamine-related genes was confirmed by semiquantitative reverse transcriptase-polymerase chain reaction analysis. During apoptosis, the genes involved in polyamine biosynthesis were downregulated, whereas those involved in polyamine catabolism were upregulated, suggesting that intracellular polyamines play a role in BCR-mediated apoptosis. Levels of intracellular putrescine, spermidine, and spermine were reduced after BCR cross-linking. These effects were prevented by concurrent CD40 stimulation, which blocked BCR-mediated apoptosis. Furthermore, addition of spermine could repress the BCR-mediated apoptosis by attenuating the mitochondrial membrane potential (Deltapsim) loss and activation of caspase-7 induced by BCR signaling. These findings strongly suggest that polyamine regulation is involved in apoptosis during B cell clonal deletion.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Antigens, CD40 (日) (読) [継承]
3.アポトーシス (apoptosis) [継承]
4. (英) B-Lymphocytes (日) (読) [継承]
5.遺伝子発現 (gene expression) [継承]
6. (英) Intracellular Fluid (日) (読) [継承]
7. (英) Mice (日) (読) [継承]
8. (英) Receptors, Antigen, B-Cell (日) (読) [継承]
9. (英) Spermine (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Experimental Cell Research ([Academic Press])
(pISSN: 0014-4827, eISSN: 1090-2422)

ISSN (任意): 0014-4827
ISSN: 0014-4827 (pISSN: 0014-4827, eISSN: 1090-2422)
Title: Experimental cell research
Title(ISO): Exp Cell Res
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2001年 4月 15日 (平成 13年 4月 15日) [継承]
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DOI (任意): 10.1006/excr.2001.5177    (→Scopusで検索) [継承]
PMID (任意): 11281655    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Microbiology and Molecular Virology, Tokyo Medical and Dental University, Tokyo, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Takeshi Nitta, Kazuei Igarashi, Atsuya Yamashita, Mikio Yamamoto and Naoki Yamamoto : Involvement of polyamines in B cell receptor-mediated apoptosis: spermine functions as a negative modulator., Experimental Cell Research, Vol.265, No.1, 174-183, 2001.
欧文冊子 ● Takeshi Nitta, Kazuei Igarashi, Atsuya Yamashita, Mikio Yamamoto and Naoki Yamamoto : Involvement of polyamines in B cell receptor-mediated apoptosis: spermine functions as a negative modulator., Experimental Cell Research, Vol.265, No.1, 174-183, 2001.

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