『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.新田 剛
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Igarashi Kazuei (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Yamamoto Naoki (日) (読)
役割 (任意):
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学籍番号 (推奨):
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題名 (必須): (英) Polyamine depletion induces apoptosis through mitochondria-mediated pathway.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Polyamines, namely putrescine, spermidine, and spermine, are essential for cell survival and proliferation. A decrease in intracellular polyamine levels is associated with apoptosis. In this study, we used inhibitors of polyamine biosynthesis to examine the effect of polyamine depletion. A combination of inhibitors of ornithine decarboxylase, S-adenosylmethionine decarboxylase, or spermidine synthase decreased intracellular polyamine levels and induced cell death in a WEHI231 murine B cell line. These cells exhibited apoptotic features including chromatin condensation and oligonucleosomal DNA fragmentation. Addition of exogenous polyamines reversed the observed features of apoptotic cell death. Similar effects were also observed in other cell lines: a human B cell line Ramos and a human T cell line Jurkat. Depletion of polyamines induced activation of caspase-3 and disruption of the mitochondrial membrane potential (Delta psi m). Inhibition of caspase activities by an inhibitor prevented the apoptotic nuclear changes but not Delta psi m disruption induced by polyamine depletion. Overexpression of Bcl-xl, an anti-apoptotic Bcl-2 family protein, completely inhibited Delta psi m disruption, caspase activation, and cell death. These results indicate that the depletion of intracellular polyamines triggers the mitochondria-mediated pathway for apoptosis, resulting in caspase activation and apoptotic cell death.  (日)    [継承]
キーワード (推奨): 1. (英) Adenosylmethionine Decarboxylase (日) (読) [継承]
2.アポトーシス (apoptosis) [継承]
3. (英) B-Lymphocytes (日) (読) [継承]
4. (英) Caspase 3 (日) (読) [継承]
5. (英) Caspase Inhibitors (日) (読) [継承]
6. (英) Caspases (日) (読) [継承]
7. (英) Cell Line (日) (読) [継承]
8. (英) Cysteine Proteinase Inhibitors (日) (読) [継承]
9. (英) Enzyme Inhibitors (日) (読) [継承]
10. (英) Humans (日) (読) [継承]
11. (英) Jurkat Cells (日) (読) [継承]
12. (英) Kinetics (日) (読) [継承]
13.ミトコンドリア (mitochondria) [継承]
14. (英) Ornithine Decarboxylase (日) (読) [継承]
15. (英) Polyamines (日) (読) [継承]
16. (英) Proto-Oncogene Proteins c-bcl-2 (日) (読) [継承]
17.シグナル伝達 (signal transduction) [継承]
18. (英) Spermidine Synthase (日) (読) [継承]
19. (英) Tumor Cells, Cultured (日) (読) [継承]
20. (英) bcl-X Protein (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Experimental Cell Research ([Academic Press])
(pISSN: 0014-4827, eISSN: 1090-2422)

ISSN (任意): 0014-4827
ISSN: 0014-4827 (pISSN: 0014-4827, eISSN: 1090-2422)
Title: Experimental cell research
Title(ISO): Exp Cell Res
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 276 [継承]
(必須): 1 [継承]
(必須): 120 128 [継承]
都市 (任意):
年月日 (必須): 西暦 2002年 5月 15日 (平成 14年 5月 15日) [継承]
URL (任意):
DOI (任意): 10.1006/excr.2002.5517    (→Scopusで検索) [継承]
PMID (任意): 11978014    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Molecular Virology, Tokyo Medical and Dental University, Tokyo 113-8519, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Takeshi Nitta, Kazuei Igarashi and Naoki Yamamoto : Polyamine depletion induces apoptosis through mitochondria-mediated pathway., Experimental Cell Research, Vol.276, No.1, 120-128, 2002.
欧文冊子 ● Takeshi Nitta, Kazuei Igarashi and Naoki Yamamoto : Polyamine depletion induces apoptosis through mitochondria-mediated pathway., Experimental Cell Research, Vol.276, No.1, 120-128, 2002.

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