『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ソシオテクノサイエンス研究部.ライフシステム部門.生命情報工学 (2006年4月1日〜2016年3月31日) [継承]
著者 (必須): 1. (英) Kikuchi Yayoi (日) 菊池 弥生 (読) きくち やよい
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2. (英) Saika Hiroko (日) 雑賀 裕子 (読) さいか ひろこ
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3.湯浅 恵造 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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4.長浜 正巳
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5.辻 明彦
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題名 (必須): (英) Isolation and Biochemical Characterization of Two Forms of RD21 from Cotyledons of Daikon Radish (Raphanus sativus).  (日)    [継承]
副題 (任意):
要約 (任意): (英) RD21 (Responsive to desiccation-21) is an Arabidopsis cysteine protease which possesses a granulin-like domain at the C-terminus. Although two forms of RD21 have been identified, consisting of an intermediate form (iRD21) containing a granulin domain and a mature form (mRD21) lacking this domain, the enzymatic properties of these enzymes remain poorly understood. In this study, mRD21 orthologue was purified to homogeneity from the cotyledons of daikon radish (Raphanus sativus). RD21 preferentially cleaved peptide bond that had an aromatic or hydrophobic amino acid at the P2 position. Furthermore, the presence of a polar amino acid at the P1 position enhanced the cleavage susceptibility of the peptide bond, although the importance of the type of amino acid residue at the P1 and P1' positions was not as significant as the residue located at the P2 position. The iRD21 was also identified as an oligomeric form by gel filtration and sedimentation analyses. The expression of RD21 mRNA was initiated by imbibition and continued at almost constant levels during germination. On the other hand, the enzyme activity increased markedly 5 days after imbibition. These results indicate that this elevation of RD21 activity is generated post-transcriptionally.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Angiotensin II (日) (読) [継承]
3. (英) Base Sequence (日) (読) [継承]
4. (英) Chromatography, Gel (日) (読) [継承]
5. (英) Cotyledon (日) (読) [継承]
6. (英) Cysteine Endopeptidases (日) (読) [継承]
7. (英) Dynorphins (日) (読) [継承]
8. (英) Hydrolysis (日) (読) [継承]
9. (英) Molecular Sequence Data (日) (読) [継承]
10. (英) Plant Proteins (日) (読) [継承]
11. (英) Protein Isoforms (日) (読) [継承]
12. (英) Raphanus (日) (読) [継承]
13. (英) Substrate Specificity (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)

ISSN (任意): 0021-924X
ISSN: 0021-924X (pISSN: 0021-924X, eISSN: 1756-2651)
Title: Journal of biochemistry
Title(ISO): J Biochem
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2008年 10月 6日 (平成 20年 10月 6日) [継承]
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DOI (任意): 10.1093/jb/mvn132    (→Scopusで検索) [継承]
PMID (任意): 18838434    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biological Science and Technology, The University of Tokushima Graduate School, 2-1 Minamijosanjima, Tokushima 770-8506, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Yayoi Kikuchi, Hiroko Saika, Keizo Yuasa, Masami Nagahama and Akihiko Tsuji : Isolation and Biochemical Characterization of Two Forms of RD21 from Cotyledons of Daikon Radish (Raphanus sativus)., The Journal of Biochemistry, Vol.144, No.6, 789-798, 2008.
欧文冊子 ● Yayoi Kikuchi, Hiroko Saika, Keizo Yuasa, Masami Nagahama and Akihiko Tsuji : Isolation and Biochemical Characterization of Two Forms of RD21 from Cotyledons of Daikon Radish (Raphanus sativus)., The Journal of Biochemistry, Vol.144, No.6, 789-798, 2008.

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