『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.神経情報医学部門.病態情報医学講座 (2004年4月1日〜) [継承]
著者 (必須): 1. (英) Yamada Hirohide (日) (読)
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2.河野 崇
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3.田中 克哉 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.麻酔・疼痛治療医学]/[徳島大学.病院.診療科.脳·神経·精神科])
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[継承]
4. (英) Yasui Sonoko (日) (読)
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[継承]
5.馬渡 一諭
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6.髙橋 章 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.予防環境栄養学])
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7.中屋 豊
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[継承]
8.大下 修造
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[継承]
題名 (必須): (英) Effects of intracellular MgADP and acidification on the inhibition of cardiac sarcolemmal ATP-sensitive potassium channels by propofol  (日)    [継承]
副題 (任意):
要約 (任意): (英) Propofol inhibits adenosine triphosphate-sensitive potassium (K(ATP)) channels, which may result in the blocking of ischemic preconditioning in the heart. During cardiac ischemia, sarcolemmal K(ATP) channel activity is regulated by the increased levels of cytosolic metabolites, such as adenosine diphosphate (ADP) and protons. However, it remains unclear whether these cytosolic metabolites modulate the inhibitory action of propofol. The aim of this study was to investigate the effects of intracellular MgADP and acidification on K(ATP) channel inhibition by propofol. We used inside-out patch-clamp configurations to investigate the effects of propofol on the activities of recombinant cardiac sarcolemmal K(ATP) channels, which are reassociated by expressed subunits, sulfonylurea receptor (SUR) 2A, and inwardly rectifying potassium channels (Kir6.2). In the absence of MgADP, propofol inhibited the SUR2A/Kir6.2 channel currents in a concentration-dependent manner, and an IC(50) of 78 microM. Increasing the intracellular MgADP concentrations to 0.1 and 0.3 mM markedly attenuated the inhibitory potency of propofol, and shifted the IC(50) to 183 and 265 microM, respectively. Moreover, decreasing the intracellular pH from 7.4 to 6.5 attenuated the inhibitory potency of propofol, and shifted the IC(50) to 277 microM. In addition, propofol-induced inhibition of truncated Kir6.2DeltaC36 currents, which form a functional channel without SUR2A, was not affected by an increase in intracellular MgADP. However, intracellular acidification (pH 6.5) significantly reduced the propofol sensitivity of Kir6.2DeltaC36 channels. Our results demonstrated that the existence of intracellular MgADP and protons attenuated the direct inhibitory potency of propofol on recombinant cardiac sarcolemmal K(ATP) channels, via SUR2A and Kir6.2 subunits, respectively.  (日)    [継承]
キーワード (推奨): 1. (英) ATP-Binding Cassette Transporters (日) (読) [継承]
2. (英) Adenosine Diphosphate (日) (読) [継承]
3. (英) Anesthetics, Intravenous (日) (読) [継承]
4. (英) Animals (日) (読) [継承]
5. (英) COS Cells (日) (読) [継承]
6. (英) Cercopithecus aethiops (日) (読) [継承]
7.心臓 (heart) [継承]
8. (英) Hydrogen-Ion Concentration (日) (読) [継承]
9. (英) KATP Channels (日) (読) [継承]
10. (英) Potassium Channel Blockers (日) (読) [継承]
11. (英) Potassium Channels (日) (読) [継承]
12. (英) Potassium Channels, Inwardly Rectifying (日) (読) [継承]
13. (英) Propofol (日) (読) [継承]
14. (英) Receptors, Drug (日) (読) [継承]
15. (英) Recombinant Proteins (日) (読) [継承]
16. (英) Sarcolemma (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Anesthesia ([社団法人 日本麻酔科学会])
(pISSN: 0913-8668, eISSN: 1438-8359)

ISSN (任意): 0913-8668
ISSN: 0913-8668 (pISSN: 0913-8668, eISSN: 1438-8359)
Title: Journal of anesthesia
Title(ISO): J Anesth
Supplier: Springer Online Journal Archive
Publisher: Springer
 (NLM Catalog  (医中誌Web  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 21 [継承]
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(必須): 472 479 [継承]
都市 (任意): 東京 (Tokyo/[日本国]) [継承]
年月日 (必須): 西暦 2007年 11月 1日 (平成 19年 11月 1日) [継承]
URL (任意): http://ci.nii.ac.jp/naid/10020022503/ [継承]
DOI (任意): 10.1007/s00540-007-0551-9    (→Scopusで検索) [継承]
PMID (任意): 18008114    (→Scopusで検索) [継承]
NAID (任意): 10020022503 [継承]
WOS (任意): 000207025700004 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Anesthesiology, Tokushima University School of Medicine, 3-18-15 Kuramoto, Tokushima, 770-8503, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hirohide Yamada, Takashi Kawano, Katsuya Tanaka, Sonoko Yasui, Kazuaki Mawatari, Akira Takahashi, Yutaka Nakaya and Shuzo Oshita : Effects of intracellular MgADP and acidification on the inhibition of cardiac sarcolemmal ATP-sensitive potassium channels by propofol, Journal of Anesthesia, Vol.21, No.4, 472-479, 2007.
欧文冊子 ● Hirohide Yamada, Takashi Kawano, Katsuya Tanaka, Sonoko Yasui, Kazuaki Mawatari, Akira Takahashi, Yutaka Nakaya and Shuzo Oshita : Effects of intracellular MgADP and acidification on the inhibition of cardiac sarcolemmal ATP-sensitive potassium channels by propofol, Journal of Anesthesia, Vol.21, No.4, 472-479, 2007.

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