『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=176259EID:176259, Map:0, LastModified:2013年6月10日(月) 14:50:08, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[石丸 直澄], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.医療創生科学部門.分子口腔医学講座 (2004年4月1日〜2015年3月31日) [継承]
著者 (必須): 1.大谷 直子
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2. (英) Imamura Yuko (日) (読)
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[継承]
3. (英) Yamakoshi Kimi (日) (読)
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4.廣田 史子
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5. (英) Nakayama Rika (日) (読)
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[継承]
6. (英) Kubo Yoshiaki (日) (読)
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7.石丸 直澄 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子病態学])
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8. (英) Takahashi Akiko (日) (読)
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9. (英) Hirao Atsushi (日) (読)
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10. (英) Shimizu Takatsune (日) (読)
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11. (英) Mann J. David (日) (読)
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12. (英) Saya Hideyuki (日) (読)
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13.林 良夫
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14.荒瀬 誠治
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15.松本 満 ([徳島大学.先端酵素学研究所.重点研究部門])
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16. (英) Kazuki Nakao (日) (読)
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17.原 英二
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題名 (必須): (英) Visualizing the dynamics of p21 (Wafl/Cip1)cyclin-dependent kinase inhibitor expression in living animals.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Although the role of p21(Waf1/Cip1) gene expression is well documented in various cell culture studies, its in vivo roles are poorly understood. To gain further insight into the role of p21(Waf1/Cip1) gene expression in vivo, we attempted to visualize the dynamics of p21(Waf1/Cip1) gene expression in living animals. In this study, we established a transgenic mice line (p21-p-luc) expressing the firefly luciferase under the control of the p21(Waf1/Cip1) gene promoter. In conjunction with a noninvasive bioluminescent imaging technique, p21-p-luc mice enabled us to monitor the endogenous p21(Waf1/Cip1) gene expression in vivo. By monitoring and quantifying the p21(Waf1/Cip1) gene expression repeatedly in the same mouse throughout its entire lifespan, we were able to unveil the dynamics of p21(Waf1/Cip1) gene expression in the aging process. We also applied this system to chemically induced skin carcinogenesis and found that the levels of p21(Waf1/Cip1) gene expression rise dramatically in benign skin papillomas, suggesting that p21(Waf1/Cip1) plays a preventative role(s) in skin tumor formation. Surprisingly, moreover, we found that the level of p21(Waf1/Cip1) expression strikingly increased in the hair bulb and oscillated with a 3-week period correlating with hair follicle cycle progression. Notably, this was accompanied by the expression of p63 but not p53. This approach, together with the analysis of p21(Waf1/Cip1) knockout mice, has uncovered a novel role for the p21(Waf1/Cip1) gene in hair development. These data illustrate the unique utility of bioluminescence imaging in advancing our understanding of the timing and, hence, likely roles of specific gene expression in higher eukaryotes.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Cyclin-Dependent Kinase Inhibitor p21 (日) (読) [継承]
3. (英) Firefly Luciferin (日) (読) [継承]
4. (英) Fluorescent Antibody Technique (日) (読) [継承]
5.遺伝子発現 (gene expression) [継承]
6. (英) Luciferases (日) (読) [継承]
7. (英) Luminescent Agents (日) (読) [継承]
8. (英) Luminescent Measurements (日) (読) [継承]
9. (英) Mice (日) (読) [継承]
10.ノックアウトマウス (knockout mice) [継承]
11. (英) Mice, Transgenic (日) (読) [継承]
12. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Proceedings of the National Academy of Sciences of the United States of America ([The National Academy of Sciences of the United States of America])
(pISSN: 0027-8424, eISSN: 1091-6490)

ISSN (任意): 0027-8424
ISSN: 0027-8424 (pISSN: 0027-8424, eISSN: 1091-6490)
Title: Proceedings of the National Academy of Sciences of the United States of America
Title(ISO): Proc Natl Acad Sci U S A
Publisher: National Academy of Sciences
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 104 [継承]
(必須): 38 [継承]
(必須): 15034 15039 [継承]
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年月日 (必須): 西暦 2007年 9月 11日 (平成 19年 9月 11日) [継承]
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DOI (任意): 10.1073/pnas.0706949104    (→Scopusで検索) [継承]
PMID (任意): 17848507    (→Scopusで検索) [継承]
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WOS (任意): 000249715100030 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Institute for Genome Research, University of Tokushima, Tokushima 770-8503, Japan. ohtani@genome.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Evaluation Studies  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) OtherID: PMC1975854  (日)    [継承]

標準的な表示

和文冊子 ● Naoko Ohtani, Yuko Imamura, Kimi Yamakoshi, Fumiko Hirota, Rika Nakayama, Yoshiaki Kubo, Naozumi Ishimaru, Akiko Takahashi, Atsushi Hirao, Takatsune Shimizu, David J. Mann, Hideyuki Saya, Yoshio Hayashi, Seiji Arase, Mitsuru Matsumoto, Nakao Kazuki and Eiji Hara : Visualizing the dynamics of p21 (Wafl/Cip1)cyclin-dependent kinase inhibitor expression in living animals., Proceedings of the National Academy of Sciences of the United States of America, Vol.104, No.38, 15034-15039, 2007.
欧文冊子 ● Naoko Ohtani, Yuko Imamura, Kimi Yamakoshi, Fumiko Hirota, Rika Nakayama, Yoshiaki Kubo, Naozumi Ishimaru, Akiko Takahashi, Atsushi Hirao, Takatsune Shimizu, David J. Mann, Hideyuki Saya, Yoshio Hayashi, Seiji Arase, Mitsuru Matsumoto, Nakao Kazuki and Eiji Hara : Visualizing the dynamics of p21 (Wafl/Cip1)cyclin-dependent kinase inhibitor expression in living animals., Proceedings of the National Academy of Sciences of the United States of America, Vol.104, No.38, 15034-15039, 2007.

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