『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=175207EID:175207, Map:0, LastModified:2012年10月10日(水) 13:59:30, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[山﨑 尚志], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨): 研究 [継承]
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.ゲノム機能研究センター (〜2008年3月31日/->組織[徳島大学.疾患プロテオゲノム研究センター]) [継承]
2.徳島大学.疾患プロテオゲノム研究センター (2008年4月1日〜2016年3月31日/->組織[徳島大学.先端酵素学研究所.プロテオゲノム研究領域]) [継承]
著者 (必須): 1. (英) Iwahashi Akihiro (日) (読)
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2. (英) Ishii Aoi (日) (読)
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3.山﨑 尚志 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.薬物治療学])
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4. (英) Hashimoto Mutsuru (日) (読)
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5. (英) Okura Kazuto (日) (読)
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6.片岡 正俊
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7.真島 英司 (アプロサイエンス)
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8.寺田 弘 ([東京理科大学])
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9.篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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題名 (必須): (英) Functionally important conserved length of C-terminal regions of yeast and bovine ADP/ATP carriers, identified by deletion mutants studies, and water accessibility of the amino acids at the C-terminal region of the yeast carrier.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Comparison of the amino acid sequence of yeast type 2 ADP/ATP carrier (yAAC2) with that of bovine type 1 AAC (bAAC1) revealed that the N- and C-terminus of yAAC2 are 15- and 6-amino acids longer, respectively, than those of bAAC1. In the present study, we focused on the difference in the C-terminal region between yAAC2 and bAAC1. Deletion of first six residues of C-terminus of yAAC did not markedly affect the function of yAAC2; however, further deletion of 1 amino acid (7th amino acid from the C-terminus) destroyed its function. On the contrary, deletion of the first amino acid residue of the C-terminus of bAAC1 caused failure of its functional expression in yeast mitochondria. Based on these results, we concluded that the 6-amino acid residue extension of the C-terminus of yAAC2 was not necessary for the function of this carrier and that the remainder of the C-terminal region of yAAC2, having a length conserved with that of bAAC1, is important for the transport function of AACs. We next prepared various single-Cys mutants in which each of 32 residues in the C-terminus of yAAC2 was replaced by a Cys residue. Since all mutants were successfully expressed in yeast mitochondria, we examined the reactivity of these cysteine residues with the membrane-impermeable sulfhydryl reagent eosin 5-maleimide (EMA). As a result, all cysteine residues that replaced the 9 continuous amino acids in Met310-Lys318 showed high reactivity with EMA regardless of the presence of carboxyatractyloside or bongkrekic acid; and so this region was concluded to be exposed to the water-accessible environment. Furthermore, based on the reactivities of cysteine residues that replaced amino acids in the sixth transmembrane segment, the probable structural features of the C-terminal region of this carrier in the presence of bongkrekic acid were discussed.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Bongkrekic Acid (日) (読) [継承]
4. (英) Cattle (日) (読) [継承]
5. (英) Eosine Yellowish-(YS) (日) (読) [継承]
6. (英) Gene Deletion (日) (読) [継承]
7. (英) Mitochondrial ADP, ATP Translocases (日) (読) [継承]
8. (英) Molecular Sequence Data (日) (読) [継承]
9. (英) Protein Conformation (日) (読) [継承]
10. (英) Saccharomyces cerevisiae Proteins (日) (読) [継承]
11. (英) Sequence Alignment (日) (読) [継承]
12. (英) Transformation, Genetic (日) (読) [継承]
発行所 (推奨): Elsevier (->組織[Elsevier Science]) [継承]
誌名 (必須): Mitochondrion (Mitochondria Research Society)
(pISSN: 1567-7249, eISSN: 1872-8278)

ISSN (任意): 1567-7249
ISSN: 1567-7249 (pISSN: 1567-7249, eISSN: 1872-8278)
Title: Mitochondrion
Title(ISO): Mitochondrion
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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都市 (任意): アムステルダム (Amsterdam/[オランダ王国]) [継承]
年月日 (必須): 西暦 2008年 3月 初日 (平成 20年 3月 初日) [継承]
URL (任意):
DOI (任意): 10.1016/j.mito.2008.01.004    (→Scopusで検索) [継承]
PMID (任意): 18313366    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima 770-8503, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Comparative Study  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Akihiro Iwahashi, Aoi Ishii, Naoshi Yamazaki, Mutsuru Hashimoto, Kazuto Okura, Masatoshi Kataoka, Eiji Majima, Hiroshi Terada and Yasuo Shinohara : Functionally important conserved length of C-terminal regions of yeast and bovine ADP/ATP carriers, identified by deletion mutants studies, and water accessibility of the amino acids at the C-terminal region of the yeast carrier., Mitochondrion, Vol.8, No.2, 196-204, 2008.
欧文冊子 ● Akihiro Iwahashi, Aoi Ishii, Naoshi Yamazaki, Mutsuru Hashimoto, Kazuto Okura, Masatoshi Kataoka, Eiji Majima, Hiroshi Terada and Yasuo Shinohara : Functionally important conserved length of C-terminal regions of yeast and bovine ADP/ATP carriers, identified by deletion mutants studies, and water accessibility of the amino acids at the C-terminal region of the yeast carrier., Mitochondrion, Vol.8, No.2, 196-204, 2008.

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