『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
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学究種別 (推奨):
組織 (推奨): 1.疾患酵素学研究センター (2007年4月1日〜2016年3月31日/->組織[徳島大学.先端酵素学研究所.次世代酵素学研究領域]) [継承]
著者 (必須): 1. (英) Jun Youn Do (日) (読)
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2. (英) Park Sun Hae (日) (読)
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3. (英) Lee Young Ji (日) (読)
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4. (英) Baek Youn Joo (日) (読)
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5. (英) Park Ki Hwan (日) 朴 煥埼 (読) ぱく はんき
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6.福井 清 ([徳島大学])
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7. (英) Kim Ho Young (日) 金 英浩 (読) きむ よんほ
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題名 (必須): (英) Positive regulation of promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by transcription factors Sp1 and NF-Y  (日)    [継承]
副題 (任意):
要約 (任意): (英) The PHGDH gene encodes the 3-phosphoglycerate dehydrogenase that catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxy pyruvate for the phosphorylated pathway of serine biosynthesis. To understand transcriptional regulation of the human PHGDH promoter, a genomic clone containing the 5'-flanking region of the PHGDH gene was isolated from a human genomic library. The 1192-bp PHGDH promoter region was cloned by PCR using the genomic DNA isolated from the PHGDH genomic clone. Sequence analysis of the promoter region exhibited several putative transcription factor binding sites for NF-Y, Sp1, GATA-1, p53, AP2, and AP1, with no TATA-box motif at an appropriate position. Transfection of a series of deletion constructs of the promoter region into HeLa cells revealed that the core positive promoter activity resided in the -276 to +1, which contains two GC-motifs for binding Sp1 and one CCAAT-motif for NF-Y. Mutational analysis and electrophoretic mobility shift assay indicated that both the proximal GC-motif and CCAAT-motif were crucial for full induction of the promoter activity. Chromatin immunoprecipitation analysis confirmed the recruitment of Sp1 and NF-Y to the promoter region in vivo. These results demonstrated that the promoter activity of the human PHGDH gene was positively regulated by the action of transcription factors Sp1 and NF-Y.  (日)    [継承]
キーワード (推奨): 1. (英) Human 3-phosphoglycerate dehydrogenase promoter (日) (読) [継承]
2. (英) Transcriptional regulation (日) (読) [継承]
3. (英) GC-motif (日) (読) [継承]
4. (英) CCAAT-motif (日) (読) [継承]
5. (英) HeLa cells (日) (読) [継承]
6. (英) Jurkat T cells (日) (読) [継承]
発行所 (推奨): Elsevier Science B.V. [継承]
誌名 (必須): Gene ([Elsevier Science B.V.])
(pISSN: 0378-1119, eISSN: 1879-0038)

ISSN (任意): 0378-1119
ISSN: 0378-1119 (pISSN: 0378-1119, eISSN: 1879-0038)
Title: Gene
Title(ISO): Gene
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 414 [継承]
(必須): 1-2 [継承]
(必須): 106 114 [継承]
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年月日 (必須): 西暦 2008年 5月 15日 (平成 20年 5月 15日) [継承]
URL (任意):
DOI (任意): 10.1016/j.gene.2008.02.018    (→Scopusで検索) [継承]
PMID (任意): 18378410    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Laboratory of Immunobiology, School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu, Republic of Korea.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Do Youn Jun, Hae Sun Park, Ji Young Lee, Joo Youn Baek, Hwan Ki Park, Kiyoshi Fukui and Young Ho Kim : Positive regulation of promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by transcription factors Sp1 and NF-Y, Gene, Vol.414, No.1-2, 106-114, 2008.
欧文冊子 ● Do Youn Jun, Hae Sun Park, Ji Young Lee, Joo Youn Baek, Hwan Ki Park, Kiyoshi Fukui and Young Ho Kim : Positive regulation of promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by transcription factors Sp1 and NF-Y, Gene, Vol.414, No.1-2, 106-114, 2008.

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