『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=172949EID:172949, Map:0, LastModified:2013年5月29日(水) 11:21:59, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[川上 竜巳], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ソシオテクノサイエンス研究部 (2006年4月1日〜2016年3月31日) [継承]
著者 (必須): 1.櫻庭 春彦
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[継承]
2. (英) Yoneda Kazunari (日) (読)
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[継承]
3. (英) Yoshihara Kumiko (日) (読)
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[継承]
4. (英) Satoh Kyoko (日) (読)
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[継承]
5.川上 竜巳 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部])
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学籍番号 (推奨):
[継承]
6.宇都 義浩 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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学籍番号 (推奨):
[継承]
7.津下 英明 (徳島文理大学健康科学研究所)
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学籍番号 (推奨):
[継承]
8. (英) Takahashi Katsuyuki (日) (読)
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9.堀 均
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[継承]
10.大島 敏久 ([九州大学])
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Sequential aldol condensation catalyzed by hyperthermophilic 2-deoxy-D-ribose-5-phosphate aldolase.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Genes encoding 2-deoxy-d-ribose-5-phosphate aldolase (DERA) homologues from two hyperthermophiles, the archaeon Pyrobaculum aerophilum and the bacterium Thermotoga maritima, were expressed individually in Escherichia coli, after which the structures and activities of the enzymes produced were characterized and compared with those of E. coli DERA. To our surprise, the two hyperthermophilic DERAs showed much greater catalysis of sequential aldol condensation using three acetaldehydes as substrates than the E. coli enzyme, even at a low temperature (25 degrees C), although both enzymes showed much less 2-deoxy-d-ribose-5-phosphate synthetic activity. Both the enzymes were highly resistant to high concentrations of acetaldehyde and retained about 50% of their initial activities after a 20-h exposure to 300 mM acetaldehyde at 25 degrees C, whereas the E. coli DERA was almost completely inactivated after a 2-h exposure under the same conditions. The structure of the P. aerophilum DERA was determined by X-ray crystallography to a resolution of 2.0 A. The main chain coordinate of the P. aerophilum enzyme monomer was quite similar to those of the T. maritima and E. coli enzymes, whose crystal structures have already been solved. However, the quaternary structure of the hyperthermophilic enzymes was totally different from that of the E. coli DERA. The areas of the subunit-subunit interface in the dimer of the hyperthermophilic enzymes are much larger than that of the E. coli enzyme. This promotes the formation of the unique dimeric structure and strengthens the hydrophobic intersubunit interactions. These structural features are considered responsible for the extremely high stability of the hyperthermophilic DERAs.  (日)    [継承]
キーワード (推奨): 1. (英) Acetaldehyde (日) (読) [継承]
2. (英) Aldehyde-Lyases (日) (読) [継承]
3. (英) Archaeal Proteins (日) (読) [継承]
4. (英) Binding Sites (日) (読) [継承]
5. (英) Catalysis (日) (読) [継承]
6. (英) Enzyme Stability (日) (読) [継承]
7. (英) Hot Temperature (日) (読) [継承]
8. (英) Hydrogen-Ion Concentration (日) (読) [継承]
9. (英) Magnetic Resonance Spectroscopy (日) (読) [継承]
10. (英) Models, Molecular (日) (読) [継承]
11.分子構造 (molecular structure) [継承]
12. (英) Protein Structure, Secondary (日) (読) [継承]
13. (英) Protein Structure, Tertiary (日) (読) [継承]
14. (英) Pyrobaculum (日) (読) [継承]
15. (英) Ribosemonophosphates (日) (読) [継承]
16.構造活性相関 (structureactivity relationship) [継承]
17. (英) Substrate Specificity (日) (読) [継承]
18. (英) Thermotoga maritima (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Applied and Environmental Microbiology ([アメリカ微生物学会])
(pISSN: 0099-2240, eISSN: 1098-5336)

ISSN (任意): 0099-2240
ISSN: 0099-2240 (pISSN: 0099-2240, eISSN: 1098-5336)
Title: Applied and environmental microbiology
Title(ISO): Appl Environ Microbiol
Publisher: American Society for Microbiology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 73 [継承]
(必須): 22 [継承]
(必須): 7427 7434 [継承]
都市 (任意):
年月日 (必須): 西暦 2007年 9月 28日 (平成 19年 9月 28日) [継承]
URL (任意):
DOI (任意): 10.1128/AEM.01101-07    (→Scopusで検索) [継承]
PMID (任意): 17905878    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Life System, Institute of Technology and Science, University of Tokushima, 2-1 Minamijosanjima-cho, Tokushima 770-8506, Japan.  (日)    [継承]
2.(英) Article.DataBankList.DataBank.DataBankName: PDB  (日)    [継承]
3.(英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: UNKNOWN  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
5.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
6.(英) OtherID: PMC2168217  (日)    [継承]

標準的な表示

和文冊子 ● Haruhiko Sakuraba, Kazunari Yoneda, Kumiko Yoshihara, Kyoko Satoh, Ryushi Kawakami, Yoshihiro Uto, Hideaki Tsuge, Katsuyuki Takahashi, Hitoshi Hori and Toshihisa Ohshima : Sequential aldol condensation catalyzed by hyperthermophilic 2-deoxy-D-ribose-5-phosphate aldolase., Applied and Environmental Microbiology, Vol.73, No.22, 7427-7434, 2007.
欧文冊子 ● Haruhiko Sakuraba, Kazunari Yoneda, Kumiko Yoshihara, Kyoko Satoh, Ryushi Kawakami, Yoshihiro Uto, Hideaki Tsuge, Katsuyuki Takahashi, Hitoshi Hori and Toshihisa Ohshima : Sequential aldol condensation catalyzed by hyperthermophilic 2-deoxy-D-ribose-5-phosphate aldolase., Applied and Environmental Microbiology, Vol.73, No.22, 7427-7434, 2007.

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