『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=172601)

EID=172601EID:172601, Map:0, LastModified:2018年2月8日(木) 14:03:01, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
3.徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.顎口腔病態制御学講座 (2004年4月1日〜) [継承]
著者 (必須): 1.森本 景之
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2.岡村 裕彦
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3.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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学籍番号 (推奨):
[継承]
4.北村 清一郎
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[継承]
5.羽地 達次
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[継承]
題名 (必須): (英) Okadaic acid induces apoptosis through double-stranded RNA-dependent protein kinase/eukaryotic initiation factor-2a pathway in human osteoblastic MG63 cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Double-stranded RNA-dependent protein kinase (PKR) is a participant in the cellular antiviral response and phosphorylates the alpha-subunit of eukaryotic translation initiation factor 2alpha (eIF-2alpha) to block protein synthesis. Treatment of human osteosarcoma cell line MG63 cells with a serine and threonine protein phosphatase inhibitor, okadaic acid, at the concentration of 100 nM, but not at 20 nM, induced apoptosis. To investigate the functional relationship between phosphatases and apoptosis, we examined the phosphorylation levels of PKR and eIF-2alpha by Western blot analysis. During treatment of cells with it at the higher concentration (100 nM), okadaic acid increased the level of phosphorylated PKR in MG63 cells, this kinase phosphorylating eIF-2alpha. However, at the lower concentration (20 nM), okadaic acid did not affect the level of phosphorylated PKR. In the cells treated with 100 nM okadaic acid, activation of NF-kappaB also occurred. Even though inhibition of translation occurred simultaneously in MG63 cells, the expression of pro-apoptotic proteins Fas and Bax was not affected by 100 nM okadaic acid in these cells. We concluded that the inhibition of translation decreased anti-apoptotic protein expression, thus resulting in apoptosis. Our results also suggest that the inhibition of the protein phosphatase activity by okadaic acid induced apoptosis in MG63 cells through PKR and eIF-2alpha.  (日)    [継承]
キーワード (推奨): 1. (英) Apoptosis (日) (読) [継承]
2. (英) Blotting, Western (日) (読) [継承]
3. (英) Cell Line, Tumor (日) (読) [継承]
4. (英) DNA (日) (読) [継承]
5. (英) Dose-Response Relationship, Drug (日) (読) [継承]
6. (英) Electrophoresis, Agar Gel (日) (読) [継承]
7. (英) Electrophoresis, Polyacrylamide Gel (日) (読) [継承]
8. (英) Eukaryotic Initiation Factor-2 (日) (読) [継承]
9. (英) Humans (日) (読) [継承]
10. (英) Microscopy, Phase-Contrast (日) (読) [継承]
11. (英) NF-kappa B (日) (読) [継承]
12. (英) Okadaic Acid (日) (読) [継承]
13. (英) Osteoblasts (日) (読) [継承]
14. (英) Phosphorylation (日) (読) [継承]
15. (英) Protein Biosynthesis (日) (読) [継承]
16. (英) Pyrazoles (日) (読) [継承]
17. (英) Pyrimidines (日) (読) [継承]
18. (英) Time Factors (日) (読) [継承]
19. (英) eIF-2 Kinase (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)

ISSN (任意): 0021-924X
ISSN: 0021-924X (pISSN: 0021-924X, eISSN: 1756-2651)
Title: Journal of biochemistry
Title(ISO): J Biochem
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2004年 10月 初日 (平成 16年 10月 初日) [継承]
URL (任意):
DOI (任意): 10.1093/jb/mvh144    (→Scopusで検索) [継承]
PMID (任意): 15625311    (→Scopusで検索) [継承]
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WOS (任意): 000226416900005 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Anatomy, School of Dentistry, The University of Tokushima, Kuramoto, Tokushima 770-8504, Japan. morimoto@dent.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hiroyuki Morimoto, Hirohiko Okamura, Kaya Yoshida, Seiichiro Kitamura and Tatsuji Haneji : Okadaic acid induces apoptosis through double-stranded RNA-dependent protein kinase/eukaryotic initiation factor-2a pathway in human osteoblastic MG63 cells, The Journal of Biochemistry, Vol.136, No.4, 433-438, 2004.
欧文冊子 ● Hiroyuki Morimoto, Hirohiko Okamura, Kaya Yoshida, Seiichiro Kitamura and Tatsuji Haneji : Okadaic acid induces apoptosis through double-stranded RNA-dependent protein kinase/eukaryotic initiation factor-2a pathway in human osteoblastic MG63 cells, The Journal of Biochemistry, Vol.136, No.4, 433-438, 2004.

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