『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=172599)

EID=172599EID:172599, Map:0, LastModified:2018年2月8日(木) 14:03:01, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
著者 (必須): 1.岡村 裕彦
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2.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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[継承]
3. (英) Sasaki Eiko (日) (読)
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[継承]
4.森本 景之
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学籍番号 (推奨):
[継承]
5.羽地 達次
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[継承]
題名 (必須): (英) Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) In this study, the expression and transcriptional regulation of the multidrug resistance-1 (MDR1) gene in multidrug-resistant SCCTF cells and -sensitive SCCKN cells derived from human squamous carcinoma were investigated. RT-PCR revealed that mdr1 mRNA was highly expressed in SCCTF cells while it was under the limit of detection in SCCKN cells. With an electrophoretic mobility shift assay using the mdr1 promoter region, a DNA-protein complex was detected strongly in SCCTF cells, but weakly in SCCKN cells. Incubation of the DNA-protein complex with an anti-NF-Y antibody caused a supershift in the migration to a position near the origin of the gel. Chromatin immunoprecipitation assay with an anti-NF-Y antibody showed that NF-Y binds to mdr1 promoter in SCCTF cells. The mdr1 promoter region including its NF-Y binding sequence was cloned into the luciferase reporter plasmid pGL3-basic vector, and this vector was used to transfect SCCTF and SCCKN cells. The luciferase assay showed that the inverted CCAAT sequence in the mdr1 promoter region is involved in the positive regulation of mdr1 promoter activity. NF-YA protein was expressed at higher levels in SCCTF cells than that in SCCKN cells. Hoechst dye staining also showed that MDR1 protein acts more effectively as an efflux pump in SCCTF cells than that in SCCKN cells.  (日)    [継承]
キーワード (推奨): 1. (英) Base Sequence (日) (読) [継承]
2. (英) CCAAT-Binding Factor (日) (読) [継承]
3. (英) Carcinoma, Squamous Cell (日) (読) [継承]
4. (英) Cell Line, Tumor (日) (読) [継承]
5. (英) DNA-Binding Proteins (日) (読) [継承]
6. (英) Drug Resistance, Neoplasm (日) (読) [継承]
7. (英) Gene Expression Regulation, Neoplastic (日) (読) [継承]
8. (英) Genes, MDR (日) (読) [継承]
9. (英) Humans (日) (読) [継承]
10. (英) Molecular Sequence Data (日) (読) [継承]
11. (英) Promoter Regions, Genetic (日) (読) [継承]
発行所 (推奨):
誌名 (必須): International Journal of Oncology (International Center for Cancer Research)
(pISSN: 1019-6439, eISSN: 1791-2423)

ISSN (任意): 1019-6439
ISSN: 1019-6439 (pISSN: 1019-6439, eISSN: 1791-2423)
Title: International journal of oncology
Title(ISO): Int J Oncol
Publisher: Spandidos Publications
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 25 [継承]
(必須): 4 [継承]
(必須): 1031 1037 [継承]
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年月日 (必須): 西暦 2004年 10月 初日 (平成 16年 10月 初日) [継承]
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PMID (任意): 15375553    (→Scopusで検索) [継承]
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WOS (任意): 000224073700027 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, 18-15, 3 Kuramoto-cho, Tokushima 770-8504, Japan. okamura@dent.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hirohiko Okamura, Kaya Yoshida, Eiko Sasaki, Hiroyuki Morimoto and Tatsuji Haneji : Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells, International Journal of Oncology, Vol.25, No.4, 1031-1037, 2004.
欧文冊子 ● Hirohiko Okamura, Kaya Yoshida, Eiko Sasaki, Hiroyuki Morimoto and Tatsuji Haneji : Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells, International Journal of Oncology, Vol.25, No.4, 1031-1037, 2004.

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