| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日)
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| 2. | 徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜)
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| ○著者 (必須): | 1. | (英) Fujita Michi (日) (読)
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| 2. | (英) Goto Kaho (日) (読)
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| 3. | 吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健支援学]/[徳島大学.歯学部.口腔保健学科.口腔保健支援学講座])
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| 4. | 岡村 裕彦
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| 5. | 森本 景之
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| 6. | (英) Kito Shinji (日) (読)
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| 7. | (英) Fukuda Jinichi (日) (読)
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| 8. | 羽地 達次
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| ○題名 (必須): | □ | (英) Okadaic acid stimulates expression of Fas receptor and Fas ligand by activation of nuclear factor kappa-B in human oral squamous carcinoma cells (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) In the present study, we used western blot and RT-PCR analysis to examine the expression of proteins and mRNAs of Fas receptor and Fas ligand in human oral squamous carcinoma SCC-25 cells treated with okadaic acid. Treatment with okadaic acid enhanced the expression of proteins and mRNAs of both Fas receptor and Fas ligand in SCC-25 cells. The amount of IkappaB-alpha in whole cell lysates decreased, while the level of NF-kappaB in nucleus increased, in the okadaic acid-treated cells. Okadaic acid-treatment also alters the cellular localization of NF-kappaB, from cytoplasm to nuclei. To investigate the activation of NF-kappaB in okadaic acid-treated SCC-25 cells, we performed electrophoretic mobility gel shift assay using nuclear extracts and the consensus oligonucleotide for NF-kappaB DNA binding site. The binding of nuclear proteins to the oligonucleotide of NF-kappaB increased when the cells had been treated with 20 nM okadaic acid for 4 h. We transfected the cells with pFLF1, which has the promoter region of Fas receptor gene containing NF-kappaB binding site. A luciferase reporter gene assay demonstrated that the activity in the cells transfected with pFLF1 and treated with 20 nM okadaic acid increased in a time-dependent manner and that the activity was more than three-fold over that in the control cells. Our results suggest that NF-kappaB activated at early stages in the okadaic acid-treated SCC-25 cells stimulated the promoter activity of Fas receptor in the cells leading to the apoptotic death of these cells. (日)
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| ○キーワード (推奨): | 1. | (英) Antigens, CD95 (日) (読)
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| 2. | (英) Carcinoma, Squamous Cell (日) (読)
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| 3. | (英) Enzyme Inhibitors (日) (読)
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| 4. | (英) Fas Ligand Protein (日) (読)
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| 5. | (英) Gene Expression Regulation, Neoplastic (日) (読)
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| 6. | (英) Humans (日) (読)
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| 7. | (英) I-kappa B Proteins (日) (読)
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| 8. | (英) Ligands (日) (読)
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| 9. | (英) Membrane Glycoproteins (日) (読)
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| 10. | (英) NF-kappa B (日) (読)
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| 11. | (英) Neoplasm Proteins (日) (読)
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| 12. | (英) Okadaic Acid (日) (読)
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| 13. | (英) Promoter Regions, Genetic (日) (読)
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| 14. | (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読)
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| 15. | (英) Tongue Neoplasms (日) (読)
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| 16. | (英) Transfection (日) (読)
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| 17. | (英) Tumor Cells, Cultured (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Oral Oncology (European Association of Oral Medicine/International Association of Oral Pathologists/International Academy of Oral Oncology)
(pISSN: 1368-8375, eISSN: 1879-0593)
| ○ISSN (任意): | □ | 1368-8375
ISSN: 1368-8375
(pISSN: 1368-8375, eISSN: 1879-0593) Title: Oral oncologyTitle(ISO): Oral OncolPublisher: Elsevier Ltd (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 40
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| ○号 (必須): | □ | 2
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| ○頁 (必須): | □ | 199 206
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2004年 2月 初日 (平成 16年 2月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/S1368-8375(03)00152-0 (→Scopusで検索)
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| ○PMID (任意): | □ | 14693245 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Histology and Oral Histology, The University of Tokushima 3-18-15, Tokushima 770-8504, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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