『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=172208EID:172208, Map:0, LastModified:2018年2月8日(木) 14:03:07, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
著者 (必須): 1.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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2.岡村 裕彦
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3. (英) Amorim Rabelo Bruna (日) (読)
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[継承]
4. (英) Ozaki Akiko (日) (読)
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[継承]
5. (英) Tanaka Hiroaki (日) (読)
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[継承]
6.森本 景之
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7.羽地 達次
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Double-stranded RNA-dependent protein kinase is required for bone calcification in MC3T3-E1 cells in vitro  (日)    [継承]
副題 (任意):
要約 (任意): (英) In this study, we demonstrated that double-stranded RNA-dependent protein kinase (PKR) is required for the calcification of osteoblasts via the signal transducers and activators of transcription 1alpha (STAT1alpha) signaling in vitro. A dominant-negative mutant PKR cDNA, in which the amino acid lysine at 296 was replaced with arginine and which does not have catalytic activity, was transfected into mouse osteoblastic MC3T3-E1 cells; thereby, we established cells that stably expressed the PKR mutant gene (PKR-K/R). Phosphorylation of PKR was not stimulated by polyinosic-polycytidylic acid in the mutant cells. The PKR-K/R mutant cells exhibited up-regulated cell growth and had low alkaline phosphatase (ALP) activity. The PKR-K/R mutant cells were not able to form bone nodules in vitro. In the PKR-K/R mutant cells, runt-related gene 2 (Runx2)-mediated transcription decreased compared with the levels in the control cells. The expression of STAT1alpha protein increased and the protein was translocated to the nucleus in the PKR-K/R mutant cells. When the expression of STAT1alpha protein in PKR mutant cells was suppressed using RNAi, the activity of Runx2-mediated transcription recovered to the control level. Our results indicate that PKR is a stimulator of Runx2 transcription and is a negative modulator of STAT1alpha expression. Our findings also suggest that PKR plays important roles in the differentiation and calcification of osteoblasts by modulating STAT1alpha and/or Runx2 expression.  (日)    [継承]
キーワード (推奨): 1. (英) Alkaline Phosphatase (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Bone and Bones (日) (読) [継承]
4. (英) Calcification, Physiologic (日) (読) [継承]
5. (英) Cell Differentiation (日) (読) [継承]
6. (英) Cell Nucleus (日) (読) [継承]
7. (英) Core Binding Factor Alpha 1 Subunit (日) (読) [継承]
8. (英) Genes, Dominant (日) (読) [継承]
9. (英) Interferon-Stimulated Gene Factor 3 (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Osteoblasts (日) (読) [継承]
12. (英) Phosphorylation (日) (読) [継承]
13. (英) Protein Transport (日) (読) [継承]
14. (英) RNA, Double-Stranded (日) (読) [継承]
15. (英) RNA, Small Interfering (日) (読) [継承]
16. (英) Signal Transduction (日) (読) [継承]
17. (英) Trans-Activators (日) (読) [継承]
18. (英) Transcription, Genetic (日) (読) [継承]
19. (英) eIF-2 Kinase (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Experimental Cell Research ([Academic Press])
(pISSN: 0014-4827, eISSN: 1090-2422)

ISSN (任意): 0014-4827
ISSN: 0014-4827 (pISSN: 0014-4827, eISSN: 1090-2422)
Title: Experimental cell research
Title(ISO): Exp Cell Res
Publisher: Elsevier Inc.
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年月日 (必須): 西暦 2005年 10月 7日 (平成 17年 10月 7日) [継承]
URL (任意):
DOI (任意): 10.1016/j.yexcr.2005.09.006    (→Scopusで検索) [継承]
PMID (任意): 16216244    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, Tokushima 770-8504, Japan. kaya@dent.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Comparative Study  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: In Vitro  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
5.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Kaya Yoshida, Hirohiko Okamura, Bruna Rabelo Amorim, Akiko Ozaki, Hiroaki Tanaka, Hiroyuki Morimoto and Tatsuji Haneji : Double-stranded RNA-dependent protein kinase is required for bone calcification in MC3T3-E1 cells in vitro, Experimental Cell Research, Vol.311, No.1, 117-125, 2005.
欧文冊子 ● Kaya Yoshida, Hirohiko Okamura, Bruna Rabelo Amorim, Akiko Ozaki, Hiroaki Tanaka, Hiroyuki Morimoto and Tatsuji Haneji : Double-stranded RNA-dependent protein kinase is required for bone calcification in MC3T3-E1 cells in vitro, Experimental Cell Research, Vol.311, No.1, 117-125, 2005.

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