『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=172173)

EID=172173EID:172173, Map:0, LastModified:2018年2月8日(木) 14:03:15, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
著者 (必須): 1. (英) Qiu Lihong (日) (読)
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2.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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3. (英) Amorim Rabelo Bruna (日) (読)
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4.岡村 裕彦
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5.羽地 達次
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題名 (必須): (英) Calyculin A stimulates the expression of TNF-a mRNA via phosphorylation of Akt in mouse osteoblastic MC3T3-E1 cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Intracellular phosphatase activity has been recognized to play a central role in signal transduction. In the present study, we investigated the effects of calyculin A, an inhibitor of protein phosphatases, on the expression of TNF-alpha mRNA and the possible signaling pathways in mouse osteoblastic MC3T3-E1 cells. The result of semiquantitative RT-PCR showed that calyculin A increased the expression of TNF-alpha mRNA in MC3T3-E1 cells. Pre-treatment of LY294002 and Wortmannin, inhibitors of PI3K, inhibited the calyculin A-stimulated TNF-alpha mRNA expression. Western blot result disclosed that calyculin A increased the phosphorylation status of Akt at Ser473. However, U0126 and SB203580, specific inhibitor of MEK1/2 and p38MAPK, respectively, had no effect on calyculin A-stimulated expression of TNF-alpha mRNA. BAY11-7085 and CAPE, inhibitors of NF-kappaB activity, did not alter the calyculin A-stimulated TNF-alpha mRNA expression. Indirect immunofluorescent study confirmed that NF-kappaB was not translocated to the nucleus by calyculin A treatment. Our present results suggest that inhibition of phosphatase activity by calyculin A stimulate the phosphorylation of Akt at Ser473 by PI3K/Akt signaling pathway, resulting in the expression TNF-alpha mRNA.  (日)    [継承]
キーワード (推奨): 1. (英) 3T3 Cells (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Enzyme Inhibitors (日) (読) [継承]
4. (英) Mice (日) (読) [継承]
5. (英) Mitogen-Activated Protein Kinases (日) (読) [継承]
6. (英) NF-kappa B (日) (読) [継承]
7. (英) Oxazoles (日) (読) [継承]
8. (英) Phosphatidylinositol 3-Kinases (日) (読) [継承]
9. (英) Proto-Oncogene Proteins c-akt (日) (読) [継承]
10. (英) RNA, Messenger (日) (読) [継承]
11. (英) Signal Transduction (日) (読) [継承]
12. (英) Tumor Necrosis Factor-alpha (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Molecular and Cellular Endocrinology ([Elsevier])
(pISSN: 0303-7207, eISSN: 1872-8057)

ISSN (任意): 0303-7207
ISSN: 0303-7207 (pISSN: 0303-7207, eISSN: 1872-8057)
Title: Molecular and cellular endocrinology
Title(ISO): Mol Cell Endocrinol
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2007年 4月 4日 (平成 19年 4月 4日) [継承]
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DOI (任意): 10.1016/j.mce.2007.03.005    (→Scopusで検索) [継承]
PMID (任意): 17482757    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Lihong Qiu, Kaya Yoshida, Bruna Rabelo Amorim, Hirohiko Okamura and Tatsuji Haneji : Calyculin A stimulates the expression of TNF-a mRNA via phosphorylation of Akt in mouse osteoblastic MC3T3-E1 cells, Molecular and Cellular Endocrinology, Vol.271, No.1-2, 38-44, 2007.
欧文冊子 ● Lihong Qiu, Kaya Yoshida, Bruna Rabelo Amorim, Hirohiko Okamura and Tatsuji Haneji : Calyculin A stimulates the expression of TNF-a mRNA via phosphorylation of Akt in mouse osteoblastic MC3T3-E1 cells, Molecular and Cellular Endocrinology, Vol.271, No.1-2, 38-44, 2007.

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