『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=172171EID:172171, Map:0, LastModified:2018年2月8日(木) 14:03:16, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
3.徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.顎口腔病態制御学講座 (2004年4月1日〜) [継承]
著者 (必須): 1.森本 景之
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2. (英) Ozaki Akiko (日) (読)
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[継承]
3.岡村 裕彦
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4.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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5. (英) Amorim Rabelo Bruna (日) (読)
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[継承]
6. (英) Tanaka Hiroaki (日) (読)
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[継承]
7.北村 清一郎
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8.羽地 達次
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題名 (必須): (英) Differntial expression of protein phosphatase type 1 isotypes and nucleolin during cell cycle arrest  (日)    [継承]
副題 (任意):
要約 (任意): (英) In the present study, we examined the expression and cytolocalization of protein phosphatase type 1 (PP1) isoforms and nucleolin in human osteoblastic cell line MG63 cells at two boundaries in the cell cycle. We treated MG63 cells with hydroxyurea and nocodazole to arrest the cells at the G(1)/S and G(2)/M boundaries, respectively. As judged from the results of Western blot analysis, PP1 isoforms were expressed differently at each boundary of the cell cycle. Nucleolin was also shown to have a different expression pattern at each boundary. In the hydroxyurea-treated cells, nucleolus-like bodies were bigger in size and decreased in number compared with those in asynchronized cells. However, the subcellular localization of PP1s and nucleolin was not changed. Anti-nucleolin antibody interacted with 110-kDa and 95-kDa proteins present in asynchronized cells and in the cells treated with hydroxyurea. Treatment of the cells with nocodazole decreased the level of the 95-kDa form of nucleolin. In the nocodazole-treated cells, it was impossible to distinguish the distribution of each protein. The phosphorylation status of nucleolin in the cell cycle arrested samples was examined by 2D-IEF-PAGE followed by Western blot analysis. In the case of asynchronized cells or hydroxyurea-treated ones, nucleolin was located at a basic isoelectric point (dephosphorylated status); whereas in the G(2)/M arrest cells, the isoelectric point of nucleolin shifted to an acidic status, indicating that nucleolin was phosphorylated. The present results indicate that PP1 and nucleolin were differently expressed at G(1)/S and G(2)/M boundaries of the cell cycle and acted in a different fashion during cell-cycle progression.  (日)    [継承]
キーワード (推奨): 1. (英) Cell Cycle (日) (読) [継承]
2. (英) Cells, Cultured (日) (読) [継承]
3. (英) Cyclin B (日) (読) [継承]
4. (英) Cyclin B1 (日) (読) [継承]
5. (英) Cyclin D (日) (読) [継承]
6. (英) Cyclins (日) (読) [継承]
7. (英) Electrophoresis, Gel, Two-Dimensional (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Hydroxyurea (日) (読) [継承]
10. (英) Isoenzymes (日) (読) [継承]
11. (英) Nocodazole (日) (読) [継承]
12. (英) Phosphoprotein Phosphatases (日) (読) [継承]
13. (英) Phosphoproteins (日) (読) [継承]
14. (英) Protein Phosphatase 1 (日) (読) [継承]
15. (英) Protein Phosphatase 2 (日) (読) [継承]
16. (英) RNA-Binding Proteins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Cell Biochemistry and Function (John Wiley & Sons, Ltd.)
(pISSN: 0263-6484, eISSN: 1099-0844)

ISSN (任意): 0263-6484
ISSN: 0263-6484 (pISSN: 0263-6484, eISSN: 1099-0844)
Title: Cell biochemistry and function
Title(ISO): Cell Biochem Funct
Publisher: Wiley
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 25 [継承]
(必須): 4 [継承]
(必須): 369 375 [継承]
都市 (任意):
年月日 (必須): 西暦 2007年 7月 初日 (平成 19年 7月 初日) [継承]
URL (任意):
DOI (任意): 10.1002/cbf.1300    (→Scopusで検索) [継承]
PMID (任意): 16329155    (→Scopusで検索) [継承]
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WOS (任意): 000248157500002 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Oral and Maxillofacial Anatomy, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan. morimoto@dent.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) MedlineDate: 2007 Jul-Aug  (日)    [継承]

標準的な表示

和文冊子 ● Hiroyuki Morimoto, Akiko Ozaki, Hirohiko Okamura, Kaya Yoshida, Bruna Rabelo Amorim, Hiroaki Tanaka, Seiichiro Kitamura and Tatsuji Haneji : Differntial expression of protein phosphatase type 1 isotypes and nucleolin during cell cycle arrest, Cell Biochemistry and Function, Vol.25, No.4, 369-375, 2007.
欧文冊子 ● Hiroyuki Morimoto, Akiko Ozaki, Hirohiko Okamura, Kaya Yoshida, Bruna Rabelo Amorim, Hiroaki Tanaka, Seiichiro Kitamura and Tatsuji Haneji : Differntial expression of protein phosphatase type 1 isotypes and nucleolin during cell cycle arrest, Cell Biochemistry and Function, Vol.25, No.4, 369-375, 2007.

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