『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=172168)

EID=172168EID:172168, Map:0, LastModified:2018年2月8日(木) 14:03:13, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
著者 (必須): 1. (英) Ozaki Akiko (日) (読)
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貢献度 (任意):
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[継承]
2.森本 景之
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[継承]
3. (英) Tanaka Hiroaki (日) (読)
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[継承]
4.岡村 裕彦
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[継承]
5.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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学籍番号 (推奨):
[継承]
6. (英) Amorim Rabelo Bruna (日) (読)
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学籍番号 (推奨):
[継承]
7.羽地 達次
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題名 (必須): (英) Okadaic acid induces phosphorylation of p65NF-κB on serine 536 adn activates NF-κB transcriptional activity in human osteoblastic MG63 cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Nuclear factor-kappa B (NF-kappaB) is an essential transcription factor in the control of expression of genes involved in cell growth, differentiation, inflammation, and neoplastic transformation. Previously, we reported that okadaic acid (OA), which is a specific inhibitor of serine/threonine protein phosphatases, induced apoptosis in cells of human osteosarcoma cell line MG63. However, to date, it is not clear whether the phosphorylation status of NF-kappaB could be affected by the treatment with OA. In this report, we demonstrate that treatment of MG63 cells with OA enhanced the phosphorylation level of NF-kappaB, as judged from the results of Western blot analysis and a lambda protein phosphatase dephosphorylation assay. The phosphorylation level of NF-kappaB was enhanced in both time- and dose-dependent manners. In the cells treated with 100 nM OA for 3 h, consequential translocation of NF-kappaB from the cytosol to the nucleus occurred. Western blotting experiments with an anti-phospho-p65NF-kappaB antibody disclosed that the NF-kappaB was phosphorylated on serine 536. Furthermore, OA stimulated the transcriptional activity of NF-kappaB in MG63 cells, as judged from the results of a luciferase assay. Our findings indicate that OA elicit phosphorylation of NF-kappaB on serine 536 in MG63 cells, resulting in the translocation of phospho-NF-kappaB to the nucleus, thereby promoting transcriptional activity of genes.  (日)    [継承]
キーワード (推奨): 1. (英) Cell Line (日) (読) [継承]
2. (英) Enzyme Inhibitors (日) (読) [継承]
3. (英) Humans (日) (読) [継承]
4. (英) Okadaic Acid (日) (読) [継承]
5. (英) Osteoblasts (日) (読) [継承]
6. (英) Phosphorylation (日) (読) [継承]
7. (英) Promoter Regions, Genetic (日) (読) [継承]
8. (英) Serine (日) (読) [継承]
9. (英) Transcription Factor RelA (日) (読) [継承]
10. (英) Transcription, Genetic (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Cellular Biochemistry ([Wiley-Liss, Inc.])
(pISSN: 0730-2312, eISSN: 1097-4644)

ISSN (任意): 0730-2312
ISSN: 0730-2312 (pISSN: 0730-2312, eISSN: 1097-4644)
Title: Journal of cellular biochemistry
Title(ISO): J Cell Biochem
Publisher: Wiley
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 99 [継承]
(必須): 5 [継承]
(必須): 1275 1284 [継承]
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年月日 (必須): 西暦 2006年 12月 1日 (平成 18年 12月 1日) [継承]
URL (任意):
DOI (任意): 10.1002/jcb.20873    (→Scopusで検索) [継承]
PMID (任意): 16795036    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima 770-8504, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Akiko Ozaki, Hiroyuki Morimoto, Hiroaki Tanaka, Hirohiko Okamura, Kaya Yoshida, Bruna Rabelo Amorim and Tatsuji Haneji : Okadaic acid induces phosphorylation of p65NF-κB on serine 536 adn activates NF-κB transcriptional activity in human osteoblastic MG63 cells, Journal of Cellular Biochemistry, Vol.99, No.5, 1275-1284, 2006.
欧文冊子 ● Akiko Ozaki, Hiroyuki Morimoto, Hiroaki Tanaka, Hirohiko Okamura, Kaya Yoshida, Bruna Rabelo Amorim and Tatsuji Haneji : Okadaic acid induces phosphorylation of p65NF-κB on serine 536 adn activates NF-κB transcriptional activity in human osteoblastic MG63 cells, Journal of Cellular Biochemistry, Vol.99, No.5, 1275-1284, 2006.

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