『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=172043EID:172043, Map:0, LastModified:2018年2月7日(水) 11:59:52, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[副研究部長]/[徳島大学.大学院医歯薬学研究部]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
著者 (必須): 1.森本 景之
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学籍番号 (推奨):
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2.岡村 裕彦
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3.羽地 達次
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) We examined the expression and cytolocalization of the protein phosphatase type 1 delta (PP1delta) isoform and nucleolin in human osteoblastic MG63 and Saos-2 cells. Cellular fractionation of MG63 cells was done and protein was prepared from each fraction. Anti-nucleolin antibody interacted with the 100- and 95-kD proteins present in the whole-cell lysate. The 100-kD protein was detected in nuclear and nucleolar fractions. The 95-kD protein was detected in cytosolic and nucleoplasmic fractions. PP1delta and nucleolin were co-localized in the nucleolus in MG63 and Saos-2 cells revealed by an immunofluorescence method. PP1delta and nucleolin were also co-immunoprecipitated with anti-nucleolin and anti-PP1delta antibodies. In the actinomycin D-treated cells, the subcellular localization of PP1delta and nucleolin was changed. Expression of PP1delta was upregulated with actinomycin D treatment. The level of 100-kD protein did not change in the actinomycin D-treated cells. However, the level of the 95-kD band increased with actinomycin D treatment. These results indicate that PP1delta was associated with nucleolin in the nucleolus of MG63 and Saos-2 cells and that nucleolin is a possible candidate substrate for PP1delta.  (日)    [継承]
キーワード (推奨): 1. (英) Cell Line (日) (読) [継承]
2. (英) Dactinomycin (日) (読) [継承]
3. (英) Humans (日) (読) [継承]
4.免疫組織化学 (immunohistochemistry) [継承]
5. (英) Isoenzymes (日) (読) [継承]
6. (英) Nuclear Proteins (日) (読) [継承]
7. (英) Osteoblasts (日) (読) [継承]
8. (英) Phosphoprotein Phosphatases (日) (読) [継承]
9. (英) Phosphoproteins (日) (読) [継承]
10. (英) Precipitin Tests (日) (読) [継承]
11. (英) Protein Phosphatase 1 (日) (読) [継承]
12. (英) RNA-Binding Proteins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Histochemistry and Cytochemistry (The Histochemical Society)
(pISSN: 0022-1554, eISSN: 1551-5044)

ISSN (任意): 0022-1554
ISSN: 0022-1554 (pISSN: 0022-1554, eISSN: 1551-5044)
Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Title(ISO): J Histochem Cytochem
Publisher: Histochemical Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 50 [継承]
(必須): 9 [継承]
(必須): 1187 1193 [継承]
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年月日 (必須): 西暦 2002年 9月 初日 (平成 14年 9月 初日) [継承]
URL (任意):
DOI (任意): 10.1177/002215540205000905    (→Scopusで検索) [継承]
PMID (任意): 12185196    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000177967500005 [継承]
Scopus (任意): 2-s2.0-0036733594 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, Tokushima, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hiroyuki Morimoto, Hirohiko Okamura and Tatsuji Haneji : Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells, The Journal of Histochemistry and Cytochemistry, Vol.50, No.9, 1187-1193, 2002.
欧文冊子 ● Hiroyuki Morimoto, Hirohiko Okamura and Tatsuji Haneji : Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells, The Journal of Histochemistry and Cytochemistry, Vol.50, No.9, 1187-1193, 2002.

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