| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日)
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| ○著者 (必須): | 1. | 羽地 達次
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 2. | (英) Nagano Toshio (日) (読)
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 3. | (英) Koide S Samuel (日) (読)
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| ○貢献度 (任意): |
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| ○題名 (必須): | □ | (英) Identification and expression of boar sperm proteins that interact with antibodies in serum from an infertile woman (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Serum designated as IS obtained from a young healthy infertile woman induced a head-to-head agglutination of ejaculated boar sperm. The immunoglobulin G (IgG) prepared from IS localized to the acrosomal region of the sperm head obtained from the corpus and cauda epididymis as determined by an indirect immunofluorescent method. The IgG interacted with a boar sperm protein with an estimated molecular weight of 45-kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoretic (SDS-PAGE) immunoblotting technique. However, the IgG did not interact with proteins extracted from sperm obtained from the testis and caput epididymis or from non-gonadal tissues including liver, kidney, spleen, muscle and serum. The IgG interacted with additional proteins of about 75- and 38-kDa present in the corpus and cauda epididymal fluids but not those in the caput epididymal fluid. The staining intensity of the 75-kDa band was reduced and that of the 38-kDa was nullified with ejaculated seminal plasma proteins. The interacting proteins were adsorbed when chromatographed on Concanavalin A Sepharose column, suggesting that they are glycoproteins. (日)
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| ○キーワード (推奨): | 1. | (英) Agglutination Tests (日) (読)
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| 2. | (英) Animals (日) (読)
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| 3. | (英) Electrophoresis, Polyacrylamide Gel (日) (読)
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| 4. | (英) Epididymis (日) (読)
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| 5. | (英) Female (日) (読)
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| 6. | (英) Fluorescent Antibody Technique (日) (読)
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| 7. | (英) Humans (日) (読)
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| 8. | (英) Immunoblotting (日) (読)
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| 9. | (英) Immunoglobulin G (日) (読)
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| 10. | (英) Infertility, Female (日) (読)
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| 11. | (英) Male (日) (読)
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| 12. | (英) Protein Biosynthesis (日) (読)
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| 13. | (英) Proteins (日) (読)
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| 14. | (英) Spermatozoa (日) (読)
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| 15. | (英) Swine (日) (読)
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| 16. | (英) Testis (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | The Journal of Experimental Zoology (Harrison, Ross G (Ross Granville)1870-1959/Brooks, William Keith, 1848-1908/Wistar Institute of Anatomy and Biology/American Society of Zoologists)
(pISSN: 0022-104X, eISSN: 1097-010X)
| ○ISSN (任意): | □ | 0022-104X
ISSN: 0022-104X
(pISSN: 0022-104X, eISSN: 1097-010X) Title: The Journal of experimental zoologyTitle(ISO): J Exp ZoolPublisher: John Wiley and Sons Inc (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 259
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 109 116
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 1991年 7月 初日 (平成 3年 7月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1002/jez.1402590113 (→Scopusで検索)
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| ○PMID (任意): | □ | 2072088 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-0026197838
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| ○researchmap (任意): |
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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