『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=171570EID:171570, Map:0, LastModified:2012年10月15日(月) 23:04:50, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[辻 明彦], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.工学部.生物工学科.生物反応工学講座 [継承]
著者 (必須): 1.辻 明彦
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2. (英) Kanie Hiroki (日) (読)
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3. (英) Makise Hirotaka (日) (読)
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4.湯浅 恵造 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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5.長浜 正巳
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6.松田 佳子
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題名 (必須): (英) Engineering of α1-antitrypsin cariants selective for subtilisin-like proprotein convertases PACE4 and PC6: Importance of the P2' residue in stable complex formation of the serpin with proprotein convertase  (日)    [継承]
副題 (任意):
要約 (任意): (英) Furin and PACE4, members of the subtilisin-like proprotein convertase (SPC) family, have been implicated in the metastatic progression of certain tumors in addition to the activation of viral coat proteins and bacterial toxins, indicating that these enzymes are potential targets for therapeutic agents. Alpha1-Antitrypsin Portland is an engineered alpha1-antitrypsin designed as a furin-specific inhibitor and has been used as a tool in the functional analysis of furin. In this work, we engineered rat alpha1-antitrypsin to create a PACE4-specific inhibitor. Substituting Arg-Arg-Arg-Arg for Ala-Val-Pro-Met(352) at P4-P1 and Ala for Leu(354) at P2' created a potent PACE4- and PC6-specific inhibitor. This variant (RRRRSA) formed an SDS- and heat-stable serpin/proteinase complex with PACE4 or PC6 and inhibited both enzyme activities. The RRRRSA variant was efficiently cleaved by furin without formation of the stable complex. This is the first report of a highly selective protein-based inhibitor of PACE4 and PC6. This inhibitor will be useful in delineating the roles of PACE4 and PC6 localized in the extracellular matrix.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Amino Acid Substitution (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Binding Sites (日) (読) [継承]
5. (英) Proprotein Convertase 5 (日) (読) [継承]
6. (英) Proprotein Convertases (日) (読) [継承]
7. (英) Protein Engineering (日) (読) [継承]
8. (英) Rats (日) (読) [継承]
9. (英) Serpins (日) (読) [継承]
10. (英) alpha 1-Antitrypsin (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Protein Engineering, Design & Selection ([Oxford University Press])
(pISSN: 1741-0126, eISSN: 1741-0134)

ISSN (任意): 1741-0126
ISSN: 1741-0126 (pISSN: 1741-0126, eISSN: 1741-0134)
Title: Protein engineering, design & selection : PEDS
Title(ISO): Protein Eng Des Sel
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 20 [継承]
(必須): 4 [継承]
(必須): 163 170 [継承]
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年月日 (必須): 西暦 2007年 3月 9日 (平成 19年 3月 9日) [継承]
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DOI (任意): 10.1093/protein/gzm007    (→Scopusで検索) [継承]
PMID (任意): 17351018    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biological Science and Technology, The University of Tokushima Graduate School, 2-1 Minamijosanjima, Tokushima 770-8506, Japan. tsuji@bio.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Akihiko Tsuji, Hiroki Kanie, Hirotaka Makise, Keizo Yuasa, Masami Nagahama and Yoshiko Matsuda : Engineering of α1-antitrypsin cariants selective for subtilisin-like proprotein convertases PACE4 and PC6: Importance of the P2' residue in stable complex formation of the serpin with proprotein convertase, Protein Engineering, Design & Selection, Vol.20, No.4, 163-170, 2007.
欧文冊子 ● Akihiko Tsuji, Hiroki Kanie, Hirotaka Makise, Keizo Yuasa, Masami Nagahama and Yoshiko Matsuda : Engineering of α1-antitrypsin cariants selective for subtilisin-like proprotein convertases PACE4 and PC6: Importance of the P2' residue in stable complex formation of the serpin with proprotein convertase, Protein Engineering, Design & Selection, Vol.20, No.4, 163-170, 2007.

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