『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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組織 (推奨): 1.徳島大学.工学部.生物工学科.生物反応工学講座 [継承]
著者 (必須): 1.長浜 正巳
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2. (英) Yamazoe Takeshi (日) (読)
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3. (英) Hara Yoshimitsu (日) (読)
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4. (英) Tani Katsuko (日) (読)
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5.辻 明彦
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6. (英) Tagaya Mitsuo (日) (読)
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題名 (必須): (英) The AAA-ATPase NVL2 is a component of pre-ribosomal particles that interacts with the DexD/H-box RNA helicase DOB1  (日)    [継承]
副題 (任意):
要約 (任意): (英) Nuclear VCP/p97-like protein 2 (NVL2) is a member of the chaperone-like AAA-ATPase family with two conserved ATP-binding modules. Our previous studies have shown that NVL2 is localized to the nucleolus by interacting with ribosomal protein L5 and may participate in ribosome synthesis, a process involving various non-ribosomal factors including chaperones and RNA helicases. Here, we show that NVL2 is associated with pre-ribosomal particles in the nucleus. Moreover, we used yeast two-hybrid and co-immunoprecipitation assays to identify an NVL2-interacting protein that could yield insights into NVL2 function in ribosome biogenesis. We found that NVL2 interacts with DOB1, a DExD/H-box RNA helicase, whose yeast homologue functions in a late stage of the 60S subunit synthesis. DOB1 can interact with a second ATP-binding module mutant of NVL2, which shows a dominant negative effect on ribosome synthesis. In contrast, it cannot interact with a first ATP-binding module mutant, which does not show the dominant negative effect. When the dominant negative mutant of NVL2 was overexpressed in cells, DOB1 appeared to remain associated with nuclear pre-ribosomal particles. Such accumulation was not observed upon overexpression of wild-type NVL2 or a nondominant-negative mutant. Taken together, our results suggest that NVL2 might regulate the association/dissociation reaction of DOB1 with pre-ribosomal particles by acting as a molecular chaperone.  (日)    [継承]
キーワード (推奨): 1. (英) Adenosine Triphosphatases (日) (読) [継承]
2. (英) Cell Line (日) (読) [継承]
3. (英) Cell Nucleus (日) (読) [継承]
4. (英) Humans (日) (読) [継承]
5. (英) Mutation (日) (読) [継承]
6. (英) Protein Binding (日) (読) [継承]
7. (英) RNA Helicases (日) (読) [継承]
8. (英) Ribosomes (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)

ISSN (任意): 0006-291X
ISSN: 0006-291X (pISSN: 0006-291X, eISSN: 1090-2104)
Title: Biochemical and biophysical research communications
Title(ISO): Biochem Biophys Res Commun
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 346 [継承]
(必須): 3 [継承]
(必須): 1075 1082 [継承]
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年月日 (必須): 西暦 2006年 6月 12日 (平成 18年 6月 12日) [継承]
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DOI (任意): 10.1016/j.bbrc.2006.06.017    (→Scopusで検索) [継承]
PMID (任意): 16782053    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan. nagahama@bio.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Masami Nagahama, Takeshi Yamazoe, Yoshimitsu Hara, Katsuko Tani, Akihiko Tsuji and Mitsuo Tagaya : The AAA-ATPase NVL2 is a component of pre-ribosomal particles that interacts with the DexD/H-box RNA helicase DOB1, Biochemical and Biophysical Research Communications, Vol.346, No.3, 1075-1082, 2006.
欧文冊子 ● Masami Nagahama, Takeshi Yamazoe, Yoshimitsu Hara, Katsuko Tani, Akihiko Tsuji and Mitsuo Tagaya : The AAA-ATPase NVL2 is a component of pre-ribosomal particles that interacts with the DexD/H-box RNA helicase DOB1, Biochemical and Biophysical Research Communications, Vol.346, No.3, 1075-1082, 2006.

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