| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院ヘルスバイオサイエンス研究部.学長裁量プロジェクト (2004年6月1日〜2015年3月31日)
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| ○著者 (必須): | 1. | 堀尾 哲也
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| 2. | (英) Basaki, Akemi (日) (読)
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| 3. | (英) Takeoka, Aya (日) (読)
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| 4. | (英) Yamato, Masayuki (日) (読)
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| ○題名 (必須): | □ | (英) Lethal level overexpression of γ-tubulin in fission yeast causes mitotic arrest. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) gamma-Tubulin is a member of the tubulin superfamily and plays essential roles in microtubule nucleation. While the level of other tubulins, alpha- and beta-tubulin, is strictly regulated in higher eukaryotes and overexpression of beta-tubulin is toxic in yeasts, gamma-tubulin can be overexpressed by fivefold in fission yeast without any obvious defect in growth. Extreme overexpression of gamma-tubulin in mammalian cells caused growth arrest; however, the exact level of gamma-tubulin and the critical level of gamma-tubulin necessary for growth defect were undetermined. We have constructed strains that over- or underexpress gamma-tubulin by placing the gamma-tubulin gene under the control of the inducible nmt1 promoter and its variants. Among these, the weakest promoter was able to produce enough gamma-tubulin to support normal growth when its expression was induced. A strain in which the gamma-tubulin gene was placed under the control of the strongest inducible promoter achieved 160-fold overexpression of gamma-tubulin and its growth was suppressed. Normal cytoplasmic microtubules were mostly lost in gamma-tubulin overexpressing cells and gamma-tubulin was accumulated around the periphery of nuclei. Many of the cells were arrested in mitosis. A small fraction of cells did proceed to undergo nuclear division; however, its process looked either significantly deterred or abnormal. Our results presented here suggest that excess gamma-tubulin disrupts the microtubule array and significantly deters the formation of the mitotic spindle, most likely because of random nucleation of microtubules from excess gamma-tubulin in the cytoplasm. (日)
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| ○キーワード (推奨): | 1. | (英) gamma-tubulin (日) ガンマチューブリン (読) がんまちゅーぶりん
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| 2. | (英) microtubule (日) 微小管 (読) びしょうかん
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| 3. | (英) mitosis (日) 分裂期 (読) ぶんれつき
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Cell Motility and the Cytoskeleton ([Wiley-Liss, Inc.])
(pISSN: 0886-1544, eISSN: 1097-0169)
| ○ISSN (任意): | □ | 0886-1544
ISSN: 0886-1544
(pISSN: 0886-1544, eISSN: 1097-0169) Title: Cell motility and the cytoskeletonTitle(ISO): Cell Motil CytoskeletonPublisher: Wiley (NLM Catalog)
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| ○巻 (必須): | □ | 44
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| ○号 (必須): | □ | 4
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| ○頁 (必須): | □ | 284 295
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 1999年 12月 初日 (平成 11年 12月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1002/(SICI)1097-0169(199912)44:4<284::AID-CM6>3.0.CO;2-I (→Scopusで検索)
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| ○PMID (任意): | □ | 10602257 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-0032738905
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| ○被引用数 (任意): | □ | 16
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| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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