『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.学長裁量プロジェクト (2004年6月1日〜2015年3月31日) [継承]
著者 (必須): 1.堀尾 哲也
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学籍番号 (推奨):
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2. (英) Basaki, Akemi (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Takeoka, Aya (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Yamato, Masayuki (日) (読)
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Lethal level overexpression of γ-tubulin in fission yeast causes mitotic arrest.  (日)    [継承]
副題 (任意):
要約 (任意): (英) gamma-Tubulin is a member of the tubulin superfamily and plays essential roles in microtubule nucleation. While the level of other tubulins, alpha- and beta-tubulin, is strictly regulated in higher eukaryotes and overexpression of beta-tubulin is toxic in yeasts, gamma-tubulin can be overexpressed by fivefold in fission yeast without any obvious defect in growth. Extreme overexpression of gamma-tubulin in mammalian cells caused growth arrest; however, the exact level of gamma-tubulin and the critical level of gamma-tubulin necessary for growth defect were undetermined. We have constructed strains that over- or underexpress gamma-tubulin by placing the gamma-tubulin gene under the control of the inducible nmt1 promoter and its variants. Among these, the weakest promoter was able to produce enough gamma-tubulin to support normal growth when its expression was induced. A strain in which the gamma-tubulin gene was placed under the control of the strongest inducible promoter achieved 160-fold overexpression of gamma-tubulin and its growth was suppressed. Normal cytoplasmic microtubules were mostly lost in gamma-tubulin overexpressing cells and gamma-tubulin was accumulated around the periphery of nuclei. Many of the cells were arrested in mitosis. A small fraction of cells did proceed to undergo nuclear division; however, its process looked either significantly deterred or abnormal. Our results presented here suggest that excess gamma-tubulin disrupts the microtubule array and significantly deters the formation of the mitotic spindle, most likely because of random nucleation of microtubules from excess gamma-tubulin in the cytoplasm.  (日)    [継承]
キーワード (推奨): 1. (英) gamma-tubulin (日) ガンマチューブリン (読) がんまちゅーぶりん [継承]
2. (英) microtubule (日) 微小管 (読) びしょうかん [継承]
3. (英) mitosis (日) 分裂期 (読) ぶんれつき [継承]
発行所 (推奨):
誌名 (必須): Cell Motility and the Cytoskeleton ([Wiley-Liss, Inc.])
(pISSN: 0886-1544, eISSN: 1097-0169)

ISSN (任意): 0886-1544
ISSN: 0886-1544 (pISSN: 0886-1544, eISSN: 1097-0169)
Title: Cell motility and the cytoskeleton
Title(ISO): Cell Motil Cytoskeleton
Publisher: Wiley
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 44 [継承]
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年月日 (必須): 西暦 1999年 12月 初日 (平成 11年 12月 初日) [継承]
URL (任意):
DOI (任意): 10.1002/(SICI)1097-0169(199912)44:4<284::AID-CM6>3.0.CO;2-I    (→Scopusで検索) [継承]
PMID (任意): 10602257    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-0032738905 [継承]
評価値 (任意):
被引用数 (任意): 16 [継承]
指導教員 (推奨):
備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Tetsuya Horio, Akemi Basaki, Aya Takeoka and Masayuki Yamato : Lethal level overexpression of γ-tubulin in fission yeast causes mitotic arrest., Cell Motility and the Cytoskeleton, Vol.44, No.4, 284-295, 1999.
欧文冊子 ● Tetsuya Horio, Akemi Basaki, Aya Takeoka and Masayuki Yamato : Lethal level overexpression of γ-tubulin in fission yeast causes mitotic arrest., Cell Motility and the Cytoskeleton, Vol.44, No.4, 284-295, 1999.

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