| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院ヘルスバイオサイエンス研究部.学長裁量プロジェクト (2004年6月1日〜2015年3月31日)
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| ○著者 (必須): | 1. | 堀尾 哲也
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| 2. | (英) Oakley, R. Berl (日) (読)
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| ○題名 (必須): | □ | (英) Human gamma-tubulin functions in fission yeast. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) gamma-Tubulin is a phylogenetically conserved component of microtubule-organizing centers that is essential for viability and microtubule function. To examine the functional conservation of gamma-tubulin, we have tested the ability of human gamma-tubulin to function in the fission yeast Schizosaccharomyces pombe. We have found that expression of a human gamma-tubulin cDNA restores viability and a near-normal growth rate to cells of S. pombe lacking endogenous gamma-tubulin. Immunofluorescence microscopy showed that these cells contained normal mitotic spindles and interphase microtubule arrays, and that human gamma-tubulin, like S. pombe gamma-tubulin, localized to spindle pole bodies, the fungal microtubule-organizing centers. These results demonstrate that human gamma-tubulin functions in fission yeast, and they suggest that in spite of the great morphological differences between the microtubule-organizing centers of humans and fission yeasts, gamma-tubulin is likely to perform the same tasks in both. They suggest, moreover, that the proteins that interact with gamma-tubulin, including, most obviously, microtubule-organizing center proteins, must also be conserved. We have also found that a fivefold overexpression of S. pombe gamma-tubulin causes no reduction in growth rates or alteration of microtubule organization. We hypothesize that the excess gamma-tubulin is maintained in the cytoplasm in a form incapable of nucleating microtubule assembly. Finally, we have found that expression of human gamma-tubulin or overexpression of S. pombe gamma-tubulin causes no significant alteration of resistance to the antimicrotubule agents benomyl, thiabendazole and nocodazole. (日)
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| ○キーワード (推奨): | 1. | (英) microtubule (日) 微小管 (読) びしょうかん
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| 2. | (英) gamma-tubulin (日) ガンマチューブリン (読) がんまちゅーぶりん
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| 3. | (英) fission yeast (日) 分裂酵母 (読) ぶんれつこうぼ
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| ○発行所 (推奨): | □ | The Rockefeller University Press
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| ○誌名 (必須): | □ | The Journal of Cell Biology (Rockefeller Institute/American Society for Cell Biology)
(pISSN: 0021-9525, eISSN: 1540-8140)
| ○ISSN (任意): | □ | 0021-9525
ISSN: 0021-9525
(pISSN: 0021-9525, eISSN: 1540-8140) Title: The Journal of cell biologyTitle(ISO): J Cell BiolPublisher: Rockefeller University Press (NLM Catalog)
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| ○巻 (必須): | □ | 126
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| ○号 (必須): | □ | 6
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| ○頁 (必須): | □ | 1465 1473
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 1994年 6月 初日 (平成 6年 6月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1083/jcb.126.6.1465 (→Scopusで検索)
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| ○PMID (任意): | □ | 8089179 (→Scopusで検索)
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| ○被引用数 (任意): | □ | 48
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Molecular Genetics, Ohio State University, Columbus 43210. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S. (日)
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| 4. | (英) OtherID: PMC2290944 (日)
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