『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=163186)

EID=163186EID:163186, Map:0, LastModified:2012年9月1日(土) 19:44:02, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[[学科長]/[徳島大学.医学部.医科栄養学科]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.学長裁量プロジェクト (2004年6月1日〜2015年3月31日) [継承]
著者 (必須): 1.堀尾 哲也
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学籍番号 (推奨):
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2. (英) Oakley, R. Berl (日) (読)
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学籍番号 (推奨):
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題名 (必須): (英) Human gamma-tubulin functions in fission yeast.  (日)    [継承]
副題 (任意):
要約 (任意): (英) gamma-Tubulin is a phylogenetically conserved component of microtubule-organizing centers that is essential for viability and microtubule function. To examine the functional conservation of gamma-tubulin, we have tested the ability of human gamma-tubulin to function in the fission yeast Schizosaccharomyces pombe. We have found that expression of a human gamma-tubulin cDNA restores viability and a near-normal growth rate to cells of S. pombe lacking endogenous gamma-tubulin. Immunofluorescence microscopy showed that these cells contained normal mitotic spindles and interphase microtubule arrays, and that human gamma-tubulin, like S. pombe gamma-tubulin, localized to spindle pole bodies, the fungal microtubule-organizing centers. These results demonstrate that human gamma-tubulin functions in fission yeast, and they suggest that in spite of the great morphological differences between the microtubule-organizing centers of humans and fission yeasts, gamma-tubulin is likely to perform the same tasks in both. They suggest, moreover, that the proteins that interact with gamma-tubulin, including, most obviously, microtubule-organizing center proteins, must also be conserved. We have also found that a fivefold overexpression of S. pombe gamma-tubulin causes no reduction in growth rates or alteration of microtubule organization. We hypothesize that the excess gamma-tubulin is maintained in the cytoplasm in a form incapable of nucleating microtubule assembly. Finally, we have found that expression of human gamma-tubulin or overexpression of S. pombe gamma-tubulin causes no significant alteration of resistance to the antimicrotubule agents benomyl, thiabendazole and nocodazole.  (日)    [継承]
キーワード (推奨): 1. (英) microtubule (日) 微小管 (読) びしょうかん [継承]
2. (英) gamma-tubulin (日) ガンマチューブリン (読) がんまちゅーぶりん [継承]
3. (英) fission yeast (日) 分裂酵母 (読) ぶんれつこうぼ [継承]
発行所 (推奨): The Rockefeller University Press [継承]
誌名 (必須): The Journal of Cell Biology (Rockefeller Institute/American Society for Cell Biology)
(pISSN: 0021-9525, eISSN: 1540-8140)

ISSN (任意): 0021-9525
ISSN: 0021-9525 (pISSN: 0021-9525, eISSN: 1540-8140)
Title: The Journal of cell biology
Title(ISO): J Cell Biol
Publisher: Rockefeller University Press
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 126 [継承]
(必須): 6 [継承]
(必須): 1465 1473 [継承]
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年月日 (必須): 西暦 1994年 6月 初日 (平成 6年 6月 初日) [継承]
URL (任意):
DOI (任意): 10.1083/jcb.126.6.1465    (→Scopusで検索) [継承]
PMID (任意): 8089179    (→Scopusで検索) [継承]
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被引用数 (任意): 48 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Molecular Genetics, Ohio State University, Columbus 43210.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.  (日)    [継承]
4.(英) OtherID: PMC2290944  (日)    [継承]

標準的な表示

和文冊子 ● Tetsuya Horio and Berl R. Oakley : Human gamma-tubulin functions in fission yeast., The Journal of Cell Biology, Vol.126, No.6, 1465-1473, 1994.
欧文冊子 ● Tetsuya Horio and Berl R. Oakley : Human gamma-tubulin functions in fission yeast., The Journal of Cell Biology, Vol.126, No.6, 1465-1473, 1994.

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