『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=163141EID:163141, Map:0, LastModified:2018年2月8日(木) 14:03:17, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座 (2007年4月1日〜) [継承]
3.徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.顎口腔病態制御学講座 (2004年4月1日〜) [継承]
著者 (必須): 1. (英) Tanaka Hiroaki (日) (読)
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2.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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3.岡村 裕彦
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4.森本 景之
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5.永田 俊彦
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6.羽地 達次
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題名 (必須): (英) Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-κB in human osteoblastic osteosarcoma MG63 cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Previously, we reported that okadaic acid, a specific inhibitor of serine/threonine protein phosphatases, induced apoptosis in human osteoblastic cells. However, it is not clear whether calyculin A, another inhibitor of protein phosphatases, would induce apoptosis in human osteoblastic cells and if so, which mechanisms are involved and whether the phosphorylation status of NF-kappaB could be affected by the treatment with calyculin A. In this report, we demonstrate that calyculin A induced apoptosis in MG63 cells, as judged by WST-8 assay, nuclear fragmentation, and DNA ladder formation. Expression of PTEN, FasL, and FasR mRNA was stimulated by calyculin A treatment in MG63 cells. Calyculin A also enhanced the phosphorylation level of NF-kappaB, as judged from the results of Western blot analysis and an in vitro dephosphorylation assay. Western blot analysis with anti-phospho-p65NF-kappaB antibody disclosed that the NF-kappaB was phosphorylated on serine 536 in cytosol and translocated into nucleus with calyculin A-treatment. The phosphorylation status of p65NF-kappaB was further confirmed by using the phosphorylation site-mutated p65NF-kappaB gene transfected into HEK293 cells. Unlike TNF-alpha, calyculin A treatment did not degraded IkappaBalpha within 10 min, while it degraded IkappaBalpha at 2-h treatment. Our findings indicate that calyculin A elicit phosphorylation of NF-kappaB on serine 536 in MG63 cells, resulting in the translocation of phospho-NF-kappaB to the nucleus, thereby promoting transcriptional activity of NF-kappaB-related genes.  (日)    [継承]
キーワード (推奨): 1. (英) Apoptosis (日) (読) [継承]
2. (英) Binding Sites (日) (読) [継承]
3. (英) Bone Neoplasms (日) (読) [継承]
4. (英) Cell Line, Tumor (日) (読) [継承]
5. (英) Cell Survival (日) (読) [継承]
6. (英) Enzyme Inhibitors (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Mutation (日) (読) [継承]
9. (英) Osteoblasts (日) (読) [継承]
10. (英) Osteosarcoma (日) (読) [継承]
11. (英) Oxazoles (日) (読) [継承]
12. (英) Phosphorylation (日) (読) [継承]
13. (英) Serine (日) (読) [継承]
14. (英) Transcription Factor RelA (日) (読) [継承]
15. (英) Transcription, Genetic (日) (読) [継承]
発行所 (推奨):
誌名 (必須): International Journal of Oncology (International Center for Cancer Research)
(pISSN: 1019-6439, eISSN: 1791-2423)

ISSN (任意): 1019-6439
ISSN: 1019-6439 (pISSN: 1019-6439, eISSN: 1791-2423)
Title: International journal of oncology
Title(ISO): Int J Oncol
Publisher: Spandidos Publications
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年月日 (必須): 西暦 2007年 8月 初日 (平成 19年 8月 初日) [継承]
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PMID (任意): 17611696    (→Scopusで検索) [継承]
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WOS (任意): 000248286000018 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima 770-8504, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hiroaki Tanaka, Kaya Yoshida, Hirohiko Okamura, Hiroyuki Morimoto, Toshihiko Nagata and Tatsuji Haneji : Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-κB in human osteoblastic osteosarcoma MG63 cells, International Journal of Oncology, Vol.31, No.2, 389-396, 2007.
欧文冊子 ● Hiroaki Tanaka, Kaya Yoshida, Hirohiko Okamura, Hiroyuki Morimoto, Toshihiko Nagata and Tatsuji Haneji : Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-κB in human osteoblastic osteosarcoma MG63 cells, International Journal of Oncology, Vol.31, No.2, 389-396, 2007.

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