『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.神経情報医学部門.情報統合医学講座 (2004年4月1日〜) [継承]
著者 (必須): 1. (英) Onda Masanori (日) (読)
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[継承]
2. (英) Nagata Satoshi (日) (読)
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[継承]
3. (英) FitzGerald J. David (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Beers Richard (日) (読)
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[継承]
5. (英) Fisher J. Robert (日) (読)
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[継承]
6. (英) Vincent J. James (日) (読)
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学籍番号 (推奨):
[継承]
7. (英) Lee B. (日) (読)
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学籍番号 (推奨):
[継承]
8.中村 教泰
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学籍番号 (推奨):
[継承]
9. (英) Hwang Jaulang (日) (読)
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学籍番号 (推奨):
[継承]
10. (英) Kreitman J. Robert (日) (読)
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学籍番号 (推奨):
[継承]
11. (英) Hassan Raffit (日) (読)
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[継承]
12. (英) Pastan Ira (日) (読)
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Characterization of the B Cell Epitopes Associated with a Truncated Form of Pseudomonas Exotoxin (PE38) Used to Make Immunotoxins for the Treatment of Cancer Patients  (日)    [継承]
副題 (任意):
要約 (任意): (英) Recombinant immunotoxins composed of an Ab Fv fragment joined to a truncated portion of Pseudomonas exotoxin A (termed PE38) have been evaluated in clinical trials for the treatment of various human cancers. Immunotoxin therapy is very effective in hairy cell leukemia and also has activity in other hemological malignancies; however, a neutralizing Ab response to PE38 in patients with solid tumors prevents repeated treatments to maximize the benefit. In this study, we analyze the murine Ab response as a model to study the B cell epitopes associated with PE38. Sixty distinct mAbs to PE38 were characterized. Mutual competitive binding of the mAbs indicated the presence of 7 major epitope groups and 13 subgroups. The competition pattern indicated that the epitopes are discrete and could not be reproduced using a computer simulation program that created epitopes out of random surface residues on PE38. Using sera from immunotoxin-treated patients, the formation of human Abs to each of the topographical epitopes was demonstrated. One epitope subgroup, E1a, was identified as the principal neutralizing epitope. The location of each epitope on PE38 was determined by preparing 41 mutants of PE38 in which bulky surface residues were mutated to either alanine or glycine. All 7 major epitope groups and 9 of 13 epitope subgroups were identified by 14 different mutants and these retained high cytotoxic activity. Our results indicate that a relatively small number of discrete immunogenic sites are associated with PE38, most of which can be eliminated by point mutations.  (日)    [継承]
キーワード (推奨): 1. (英) ADP Ribose Transferases (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Antibodies, Monoclonal (日) (読) [継承]
4. (英) Antibody Formation (日) (読) [継承]
5.細菌毒素 (bacterial toxins) [継承]
6. (英) Enzyme-Linked Immunosorbent Assay (日) (読) [継承]
7. (英) Epitope Mapping (日) (読) [継承]
8. (英) Epitopes, B-Lymphocyte (日) (読) [継承]
9. (英) Exotoxins (日) (読) [継承]
10. (英) Humans (日) (読) [継承]
11. (英) Immunoglobulin Fragments (日) (読) [継承]
12. (英) Immunotoxins (日) (読) [継承]
13. (英) Mice (日) (読) [継承]
14. (英) Neoplasms (日) (読) [継承]
15. (英) Pseudomonas (日) (読) [継承]
16. (英) Recombinant Fusion Proteins (日) (読) [継承]
17. (英) Virulence Factors (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Immunology ([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)

ISSN (任意): 0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 177 [継承]
(必須): 12 [継承]
(必須): 8822 8834 [継承]
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年月日 (必須): 西暦 2006年 12月 15日 (平成 18年 12月 15日) [継承]
URL (任意):
DOI (任意): 10.4049/jimmunol.177.12.8822    (→Scopusで検索) [継承]
PMID (任意): 17142785    (→Scopusで検索) [継承]
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WOS (任意): 000243416800064 [継承]
Scopus (任意): 2-s2.0-33845457606 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Bethesda, MD 20892, USA.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, N.I.H., Extramural  (日)    [継承]

標準的な表示

和文冊子 ● Masanori Onda, Satoshi Nagata, David J. FitzGerald, Richard Beers, Robert J. Fisher, James J. Vincent, B. Lee, Michihiro Nakamura, Jaulang Hwang, Robert J. Kreitman, Raffit Hassan and Ira Pastan : Characterization of the B Cell Epitopes Associated with a Truncated Form of Pseudomonas Exotoxin (PE38) Used to Make Immunotoxins for the Treatment of Cancer Patients, The Journal of Immunology, Vol.177, No.12, 8822-8834, 2006.
欧文冊子 ● Masanori Onda, Satoshi Nagata, David J. FitzGerald, Richard Beers, Robert J. Fisher, James J. Vincent, B. Lee, Michihiro Nakamura, Jaulang Hwang, Robert J. Kreitman, Raffit Hassan and Ira Pastan : Characterization of the B Cell Epitopes Associated with a Truncated Form of Pseudomonas Exotoxin (PE38) Used to Make Immunotoxins for the Treatment of Cancer Patients, The Journal of Immunology, Vol.177, No.12, 8822-8834, 2006.

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