『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.医学部.保健学科.検査技術科学専攻.形態系検査学講座 [継承]
2.大学院ヘルスバイオサイエンス研究部.プロテオミクス医科学部門.生体制御医学講座 (2004年4月1日〜) [継承]
著者 (必須): 1.梅野 真由美
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2.新家 利一
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3.佐藤 陽一 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品情報学])
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4. (英) Yang Xin-Jun (日) (読)
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5.馬場 嘉信
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6. (英) Iwamoto Teruaki (日) (読)
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7.中堀 豊
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[継承]
題名 (必須): (英) A rapid and simple system of detecting deletions on the Y chromosome related with male infertility using multiplex PCR  (日)    [継承]
副題 (任意):
要約 (任意): (英) Around 10% of males with idiopathic azoospermia or oligozoospermia, which are important causes of male infertility, have partial deletions on the long arm of the Y chromosome. To develop a rapid and accurate detection system for screening major Y deletions found in infertile men, we developed a multiplex PCR system that can simultaneously amplify five loci on the Y chromosome, SRY, AMELY, DBY, RBMY, DAZ and one locus on the X chromosome, AMELX. The size of the PCR products was designed to increase gradually from the distal Yp to the distal Yq. Our system could detect deletions of three major candidate regions for the azoospermic factor, AZFa, AZFb and AZFc on the Y chromosome together with sex. The gradual increase in the size of the PCR products was convenient for imaging the location of deletions on the Y chromosome. Moreover, the multiplex PCR system was combined with microchip-based electrophoresis, and the PCR products derived from each locus were separated within 4 min. Our system is useful for screening Y chromosomes bearing the structural anomalies including three major AZF deletions found among azoospermic or oligozoospermic males.  (日)    [継承]
キーワード (推奨): 1. (英) Base Sequence (日) (読) [継承]
2. (英) Case-Control Studies (日) (読) [継承]
3. (英) Chromosome Deletion (日) (読) [継承]
4. (英) Chromosomes, Human, Y (日) (読) [継承]
5. (英) DNA Primers (日) (読) [継承]
6. (英) Electrophoresis (日) (読) [継承]
7. (英) Genetic Loci (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Male (日) (読) [継承]
10. (英) Oligospermia (日) (読) [継承]
11. (英) Polymerase Chain Reaction (日) (読) [継承]
12. (英) Seminal Plasma Proteins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Medical Investigation : JMI ([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)

ISSN (任意): 1343-1420
ISSN: 1343-1420 (pISSN: 1343-1420, eISSN: 1349-6867)
Title: The journal of medical investigation : JMI
Title(ISO): J Med Invest
Supplier: 徳島大学
Publisher: Tokushima Daigaku Igakubu
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 53 [継承]
(必須): 1,2 [継承]
(必須): 147 152 [継承]
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年月日 (必須): 西暦 2006年 2月 初日 (平成 18年 2月 初日) [継承]
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DOI (任意): 10.2152/jmi.53.147    (→Scopusで検索) [継承]
PMID (任意): 16538008    (→Scopusで検索) [継承]
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機関リポジトリ : 110800 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Evaluation Studies  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Mayumi Umeno, Toshikatsu Shinka, Youichi Sato, Xin-Jun Yang, Yoshinobu Baba, Teruaki Iwamoto and Yutaka Nakahori : A rapid and simple system of detecting deletions on the Y chromosome related with male infertility using multiplex PCR, The Journal of Medical Investigation : JMI, Vol.53, No.1,2, 147-152, 2006.
欧文冊子 ● Mayumi Umeno, Toshikatsu Shinka, Youichi Sato, Xin-Jun Yang, Yoshinobu Baba, Teruaki Iwamoto and Yutaka Nakahori : A rapid and simple system of detecting deletions on the Y chromosome related with male infertility using multiplex PCR, The Journal of Medical Investigation : JMI, Vol.53, No.1,2, 147-152, 2006.

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