| ○種別 (必須): | □ | 学術論文 (審査論文)
| [継承] |
| ○言語 (必須): | □ | 英語
| [継承] |
| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
| [継承] |
| ○カテゴリ (推奨): | □ | 研究
| [継承] |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Kubota Makoto (日) 久保田 眞 (読) くぼた まこと
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 2. | 長谷川 敬展 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 3. | (英) Nakakura Takashi (日) 中倉 敬 (読) なかくら たかし
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 4. | (英) Tanii Haruna (日) 谷井 はるな (読) たにいはるな
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | (英) Suzuki Masakazu (日) 鈴木 雅一 (読) すずきまさかず
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 6. | (英) Tanaka Shigeyasu (日) 田中 滋康 (読) たなかしげやす
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) Molecular and cellular characterization of a new aquaporin, AQP-x5, specifically expressed in the small granular glands of Xenopus skin. (日)
| [継承] |
| ○副題 (任意): |
| ○要約 (任意): | □ | (英) A new toad aquaporin (AQP) cDNA was cloned from a cDNA library constructed from the ventral skin of Xenopus laevis. This AQP (Xenopus AQP-x5) consisted of 273 amino acid residues with a high sequence homology to mammalian AQP5. The predicted amino acid sequence contained the two conserved Asn-Pro-Ala motifs found in all major intrinsic protein (MIP) family members and six putative transmembrane domains. The sequence also contained a mercurial-sensitive cysteine and a putative phosphorylation motif site for protein kinase A at Ser-257. The swelling assay using Xenopus oocytes revealed that AQP-x5 facilitated water permeability. Expression of AQP-x5 mRNA was restricted to the skin, brain, lungs and testes. Immunofluorescence and immunoelectron microscopical studies using an anti-peptide antibody (ST-156) against the C-terminal region of the AQP-x5 protein revealed the presence of immunopositive cells in the skin, with the label predominately localized in the apical plasma membrane of the secretory cells of the small granular glands. These glands are unique both in being close to the epidermal layer of the skin and in containing mitochondria-rich cells with vacuolar H+-ATPase dispersed among its secretory cells. Results from immunohistochemical experiments on the mucous or seromucous glands of several other anurans verified this result. We conclude that the presence of AQP-x5 in the apical plasma membrane of the small granular glands suggests its involvement in water secretion from the skins. The physiological roles of the AQP-x5 protein in the small or mucous glands are discussed. (日)
| [継承] |
| ○キーワード (推奨): | 1. | (英) Amino Acid Sequence (日) (読)
| [継承] |
| 2. | (英) Animals (日) (読)
| [継承] |
| 3. | (英) Anura (日) (読)
| [継承] |
| 4. | (英) Aquaporin 5 (日) (読)
| [継承] |
| 5. | (英) Base Sequence (日) (読)
| [継承] |
| 6. | (英) Brain (日) (読)
| [継承] |
| 7. | (英) Cloning, Molecular (日) (読)
| [継承] |
| 8. | (英) Gene Library (日) (読)
| [継承] |
| 9. | (英) Lung (日) (読)
| [継承] |
| 10. | (英) Male (日) (読)
| [継承] |
| 11. | (英) Molecular Sequence Data (日) (読)
| [継承] |
| 12. | (英) Oocytes (日) (読)
| [継承] |
| 13. | (英) Protein Structure, Tertiary (日) (読)
| [継承] |
| 14. | (英) RNA, Messenger (日) (読)
| [継承] |
| 15. | (英) Secretory Vesicles (日) (読)
| [継承] |
| 16. | (英) Sequence Analysis, Protein (日) (読)
| [継承] |
| 17. | (英) Sequence Homology, Amino Acid (日) (読)
| [継承] |
| 18. | (英) Skin (日) (読)
| [継承] |
| 19. | (英) Testis (日) (読)
| [継承] |
| 20. | (英) Water (日) (読)
| [継承] |
| 21. | (英) Xenopus Proteins (日) (読)
| [継承] |
| 22. | (英) Xenopus laevis (日) (読)
| [継承] |
| ○発行所 (推奨): | □ | The Company of Biologists Limited
| [継承] |
| ○誌名 (必須): | □ | The Journal of Experimental Biology ([The Company of Biologists Limited])
(pISSN: 0022-0949, eISSN: 1477-9145)
| ○ISSN (任意): | □ | 0022-0949
ISSN: 0022-0949
(pISSN: 0022-0949, eISSN: 1477-9145) Title: The Journal of experimental biologyTitle(ISO): J Exp BiolPublisher: Company of Biologists (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 209
| [継承] |
| ○号 (必須): | □ | 16
| [継承] |
| ○頁 (必須): | □ | 3199 3208
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2006年 8月 初日 (平成 18年 8月 初日)
| [継承] |
| ○URL (任意): |
| ○DOI (任意): | □ | 10.1242/jeb.02351 (→Scopusで検索)
| [継承] |
| ○PMID (任意): | □ | 16888067 (→Scopusで検索)
| [継承] |
| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Biology, Faculty of Science, Shizuoka University, Ohya 836, Shizuoka 422-8529, Japan. (日)
| [継承] |
| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
| [継承] |
| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
| [継承] |