| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○学究種別 (推奨): |
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| ○著者 (必須): | 1. | 長谷川 敬展 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])
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| 2. | (英) Suzuki Masakazu (日) 鈴木 雅一 (読) すずき まさかず
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| 3. | (英) Tanaka Shigeyasu (日) 田中 滋康 (読) たなか しげやす
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| ○題名 (必須): | □ | (英) Immunocytochemical studies on translocation of phosphorylated aquaporin-h2 protein in granular cells of the frog urinary bladder before and after stimulation with vasotocin. (日)
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| ○要約 (任意): | □ | (英) We have generated a specific antibody against phosphorylated aquaporin-h2 (pAQP-h2) protein to investigate the role of phosphorylation in the translocation of AQP-h2 protein within the granule cells of the urinary bladder of the frog (Hyla japonica). The antibody was generated against a synthetic peptide (ST-160) corresponding to amino acids 255-268, with a phosphorylated Ser-262, a residue that is putatively phosphorylated by protein A kinase. Using this antibody, we found, by Western blot analysis, that phosphorylation of the AQP-h2 protein rapidly increased within 2 min after vasotocin (AVT) stimulation and remained at a higher than normal level for 15 min. Moreover, quantitative immunoelectron microscopy indicated that the location of the AQP-h2 protein dramatically changed after AVT stimulation. Before stimulation, pAQP-h2 protein was localized in only a small number of intracellular vesicles near the nucleus of the granular cells, whereas the labeling density of the intracellular vesicles and the apical membrane rapidly increased after stimulation. This finding was also confirmed by the results of an immunofluorescence study. Thus, phosphorylation of AQP-h2 protein seems to be essential for translocation of the protein from the cytoplasmic pool to the apical plasma membrane of the granular cells in frog urinary bladder. (日)
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| ○キーワード (推奨): | 1. | (英) Amino Acid Sequence (日) (読)
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| 2. | (英) Animals (日) (読)
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| 3. | (英) Antibodies (日) (読)
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| 4. | (英) Antibody Specificity (日) (読)
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| 5. | (英) Anura (日) (読)
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| 6. | (英) Aquaporins (日) (読)
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| 7. | (英) Blotting, Western (日) (読)
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| 8. | (英) Enzyme-Linked Immunosorbent Assay (日) (読)
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| 9. | (英) Fluorescent Antibody Technique (日) (読)
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| 10. | (英) Immunohistochemistry (日) (読)
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| 11. | (英) Microscopy, Immunoelectron (日) (読)
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| 12. | (英) Molecular Sequence Data (日) (読)
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| 13. | (英) Peptide Fragments (日) (読)
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| 14. | (英) Phosphorylation (日) (読)
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| 15. | (英) Urinary Bladder (日) (読)
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| 16. | (英) Vasotocin (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Cell and Tissue Research ([Springer-Verlag])
(pISSN: 0302-766X, eISSN: 1432-0878)
| ○ISSN (任意): | □ | 0302-766X
ISSN: 0302-766X
(pISSN: 0302-766X, eISSN: 1432-0878) Title: Cell and tissue researchTitle(ISO): Cell Tissue ResSupplier: Springer Online Journal ArchivePublisher: Springer (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 322
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 407 415
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| ○年月日 (必須): | □ | 西暦 2005年 12月 初日 (平成 17年 12月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s00441-005-0037-8 (→Scopusで検索)
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| ○PMID (任意): | □ | 16047161 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Biology, Faculty of Science, Shizuoka University, Ohya 836, Shizuoka, 422-8529, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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