『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=159567EID:159567, Map:0, LastModified:2014年2月28日(金) 13:55:25, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[長谷川 敬展], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Tanii Haruna (日) 谷井 はるな (読) たにいはるな
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2.長谷川 敬展 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生理学])
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3. (英) Hirakawa Noboru (日) 平川 昇 (読) ひらかわのぼる
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4. (英) Suzuki Masakazu (日) 鈴木 雅一 (読) すずきまさかず
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5. (英) Tanaka Shigeyasu (日) 田中 滋康 (読) たなかしげやす
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題名 (必須): (英) Molecular and cellular characterization of a water-channel protein, AQP-h3, specifically expressed in the frog ventral skin.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Two cDNAs encoding frog aquaporin (AQP) were cloned from a cDNA library constructed for the ventral skin of the tree frog, Hyla japonica and sequenced. One AQP (Hyla AQP-h1) consisted of 271 amino-acid residues with high homology to toad AQP-t1, Rana CHIP28 (AQP1), and rat AQP1. The other AQP (AQP-h3) consisted of 271 amino-acid residues with higher homology to mammalian AQP2 than to mammalian AQP3. The predicted amino-acid sequence contained the conserved two NPA motifs found in all MIP family members and the putative six transmembrane domains. The sequence also confers mercurial sensitivity, which is common to all the AQPs except AQP0, AQP4 and AQP7. Potential N-glycosylation sites were present at Asn-44 in AQP-h1, and at Asn-124 and Asn-125 in AQP-h3. In addition, AQP-h3 had a putative phosphorylation site by protein kinase A at Ser-255, which is identical to mammalian AQP2. In swelling assays using Xenopus oocytes, AQP-h1 facilitates water permeability, whereas AQP-h3 displayed weak water permeability. Searching for the expression of these two AQP mRNAs revealed that AQP-h1 was expressed in most tissues, whereas AQP-h3 was observed only in the ventral skin. An antibody (ST-141) against the C-terminal peptide of the AQP-h3 protein recognized a 29.0 kDa-protein with a molecular mass close to that of the Hyla AQP-h3 protein and immunostained predominantly in the abdominal pelvic skin. In pelvic skin, the label for AQP-h3 was more intense in the upper layer of the stratum granulosum and was localized to both the apical and basolateral plasma membranes of the principal cells. These findings suggest that Hyla AQP-h3 plays a pivotal role in constitutively absorbing water from ventral pelvic skin.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Anura (日) (読) [継承]
3. (英) Aquaporins (日) (読) [継承]
4. (英) Base Sequence (日) (読) [継承]
5. (英) DNA, Complementary (日) (読) [継承]
6. (英) Gene Expression (日) (読) [継承]
7. (英) Gene Expression Regulation (日) (読) [継承]
8. (英) Gene Library (日) (読) [継承]
9. (英) Molecular Sequence Data (日) (読) [継承]
10. (英) Oocytes (日) (読) [継承]
11. (英) Pelvis (日) (読) [継承]
12. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
13. (英) Sequence Analysis, DNA (日) (読) [継承]
14. (英) Skin (日) (読) [継承]
15. (英) Xenopus (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Membrane Biology ([Springer-Verlag])
(pISSN: 0022-2631, eISSN: 1432-1424)

ISSN (任意): 0022-2631
ISSN: 0022-2631 (pISSN: 0022-2631, eISSN: 1432-1424)
Title: The Journal of membrane biology
Title(ISO): J Membr Biol
Supplier: Springer Online Journal Archive
Publisher: Springer
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年月日 (必須): 西暦 2002年 7月 1日 (平成 14年 7月 1日) [継承]
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DOI (任意): 10.1007/s00232-001-0172-4    (→Scopusで検索) [継承]
PMID (任意): 12172646    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Comparative Study  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Haruna Tanii, Takahiro Hasegawa, Noboru Hirakawa, Masakazu Suzuki and Shigeyasu Tanaka : Molecular and cellular characterization of a water-channel protein, AQP-h3, specifically expressed in the frog ventral skin., The Journal of Membrane Biology, Vol.188, No.1, 43-53, 2002.
欧文冊子 ● Haruna Tanii, Takahiro Hasegawa, Noboru Hirakawa, Masakazu Suzuki and Shigeyasu Tanaka : Molecular and cellular characterization of a water-channel protein, AQP-h3, specifically expressed in the frog ventral skin., The Journal of Membrane Biology, Vol.188, No.1, 43-53, 2002.

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