『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=154818)

EID=154818EID:154818, Map:0, LastModified:2018年12月18日(火) 15:06:51, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[木戸 博], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.分子酵素学研究センター (〜2007年3月31日/->組織[徳島大学.疾患酵素学研究センター]) [継承]
著者 (必須): 1. (英) Yamaguchi Toru (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
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2.木戸 博 ([徳島大学.先端酵素学研究所.重点研究部門])
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[継承]
3. (英) Kitazawa Riko (日) (読)
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[継承]
4. (英) Kitazawa Sohei (日) (読)
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学籍番号 (推奨):
[継承]
5. (英) Fukase Masaaki (日) (読)
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学籍番号 (推奨):
[継承]
6. (英) Katunuma Nobuhiko (日) (読)
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学籍番号 (推奨):
[継承]
題名 (必須): (英) A Membrane-Bound Metallo-Endopeptidase from Rat Kidney: Its Immunological Characterization,  (日)    [継承]
副題 (任意):
要約 (任意): (英) The structure and location of a membrane-bound metallo-endopeptidase, previously purified from rat kidney [Yamaguchi et al. (1991) Eur. J. Biochem. 200, 563-571], were examined by immunochemical and immunohistochemical methods with a rabbit polyclonal antibody against the purified enzyme. On treatment with endoglycosidase F, the subunit of the purified enzyme (molecular mass = 88 kDa) was converted to a smaller form (78.5 kDa), indicating that the enzyme contained at least 11% N-linked carbohydrate. Treatment of kidney membranes with papain resulted in release of the enzyme, as shown by Western blotting analysis of the solubilized fraction. Immunoassays of rat tissues showed that only the kidney, and small and large intestine expressed significant amounts of the antigen. Moreover, immunohistochemical studies showed that the antigen was confined to the luminal surfaces of the proximal renal tubules and the intestinal villi. Thus, like another kidney membrane metallo-endopeptidase, meprin [Kounnas et al. (1991) J. Biol. Chem. 266, 17350-17357], the purified enzyme is shown to be a glycoprotein that is probably anchored in the plasma membrane, and located in the luminal surface of microvillar membranes of the kidney and intestine. These results indicate that our enzyme and meprin have clear structural and topological similarities.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Antibodies (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) Cell Membrane (日) (読) [継承]
5. (英) Electrophoresis, Polyacrylamide Gel (日) (読) [継承]
6. (英) Immunoenzyme Techniques (日) (読) [継承]
7.免疫組織化学 (immunohistochemistry) [継承]
8. (英) Intestines (日) (読) [継承]
9. (英) Kidney (日) (読) [継承]
10. (英) Kidney Tubules, Proximal (日) (読) [継承]
11. (英) Male (日) (読) [継承]
12. (英) Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase (日) (読) [継承]
13. (英) Metalloendopeptidases (日) (読) [継承]
14. (英) Microvilli (日) (読) [継承]
15. (英) Rats (日) (読) [継承]
16. (英) Rats, Wistar (日) (読) [継承]
17. (英) Tiopronin (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)

ISSN (任意): 0021-924X
ISSN: 0021-924X (pISSN: 0021-924X, eISSN: 1756-2651)
Title: Journal of biochemistry
Title(ISO): J Biochem
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 1993年 4月 3日 (平成 5年 4月 3日) [継承]
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PMID (任意): 8486602    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Comparative Study  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Toru Yamaguchi, Hiroshi Kido, Riko Kitazawa, Sohei Kitazawa, Masaaki Fukase and Nobuhiko Katunuma : A Membrane-Bound Metallo-Endopeptidase from Rat Kidney: Its Immunological Characterization,, The Journal of Biochemistry, Vol.113, No.3, 299-303, 1993.
欧文冊子 ● Toru Yamaguchi, Hiroshi Kido, Riko Kitazawa, Sohei Kitazawa, Masaaki Fukase and Nobuhiko Katunuma : A Membrane-Bound Metallo-Endopeptidase from Rat Kidney: Its Immunological Characterization,, The Journal of Biochemistry, Vol.113, No.3, 299-303, 1993.

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