『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.上野 明道
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[継承]
2. (英) Miwa Yoshihiro (日) (読)
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3.三好 圭子 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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4.堀口 大吾 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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学籍番号 (推奨):
[継承]
5. (英) Inoue Hideo (日) (読)
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6. (英) Ruspita Intan (日) INTAN RUSPITA (読) いんたん るすぴた
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学籍番号 (推奨): **** [ユーザ]
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7.阿部 佳織
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学籍番号 (推奨): **** [ユーザ]
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8.山下 菊治
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9. (英) Hayashi Eiji (日) 林 敬治 (読) はやし けいじ
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学籍番号 (推奨): **** [ユーザ]
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10.野間 隆文 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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[継承]
題名 (必須): (英) Constitutive expression of thrombospondin 1 in MC3T3-E1 osteoblastic cells inhibits mineralization  (日)    [継承]
副題 (任意):
要約 (任意): (英) Thrombospondin 1 (TSP1) is a multifunctional extracellular glycoprotein present mainly in the fetal and adult skeleton. Although an inhibitory effect of TSP1 against pathological mineralization in cultured vascular pericytes has been shown, its involvement in physiological mineralization by osteoblasts is still unknown. To determine the role of TSP1 in biomineralization, mouse osteoblastic MC3T3-E1 cells were cultured in the presence of antisense phosphorothioate oligodeoxynucleotides complementary to the TSP1 sequence. The 18- and 24-mer antisense oligonucleotides caused concentration-dependent increases in the number of mineralized nodules, acid-soluble calcium deposition in the cell/matrix layer, and alkaline phosphatase activity within 9 days, without affecting cell proliferation. The corresponding sense or scrambled oligonucleotides did not affect these parameters. In the antisense oligonucleotide-treated MC3T3-E1 cells, thickened extracellular matrix, well-developed cell processes, increased intracellular organelles, and collagen fibril bundles were observed. On the other hand, the addition of TSP1 to the culture decreased the production of a mineralized matrix by MC3T3-E1 cells. Furthermore, MC3T3-E1 clones overexpressing mouse TSP1 were established and assayed for TSP1 protein and their capacity to mineralize. TSP1 dose-dependently inhibited mineralization by these cells both in vitro and in vivo. These results indicate that TSP1 functions as an inhibitory regulator of bone mineralization and matrix production by osteoblasts to sustain bone homeostasis.  (日)    [継承]
キーワード (推奨): 1. (英) Alkaline Phosphatase (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Antibodies, Monoclonal (日) (読) [継承]
4. (英) Bone Matrix (日) (読) [継承]
5. (英) Calcification, Physiologic (日) (読) [継承]
6. (英) Cell Differentiation (日) (読) [継承]
7. (英) Cell Line (日) (読) [継承]
8. (英) Cell Proliferation (日) (読) [継承]
9. (英) Cell Transplantation (日) (読) [継承]
10. (英) Cells, Cultured (日) (読) [継承]
11. (英) Down-Regulation (日) (読) [継承]
12. (英) Female (日) (読) [継承]
13. (英) Gene Expression (日) (読) [継承]
14. (英) Mice (日) (読) [継承]
15. (英) Oligonucleotides, Antisense (日) (読) [継承]
16. (英) Osteoblasts (日) (読) [継承]
17. (英) Osteocalcin (日) (読) [継承]
18. (英) RNA, Messenger (日) (読) [継承]
19. (英) Recombinant Proteins (日) (読) [継承]
20. (英) Thrombospondin 1 (日) (読) [継承]
発行所 (推奨): (英) Inter Science (日) (読) [継承]
誌名 (必須): Journal of Cellular Physiology ([Wiley-Liss, Inc.])
(pISSN: 0021-9541, eISSN: 1097-4652)

ISSN (任意): 0021-9541
ISSN: 0021-9541 (pISSN: 0021-9541, eISSN: 1097-4652)
Title: Journal of cellular physiology
Title(ISO): J. Cell. Physiol.
Publisher: Wiley
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 209 [継承]
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都市 (任意):
年月日 (必須): 西暦 2006年 11月 初日 (平成 18年 11月 初日) [継承]
URL (任意): http://www3.interscience.wiley.com/cgi-bin/abstract/112737855/ABSTRACT?CRETRY=1&SRETRY=0 [継承]
DOI (任意): 10.1002/jcp.20735    (→Scopusで検索) [継承]
PMID (任意): 16883596    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Akemichi Ueno, Yoshihiro Miwa, Keiko Miyoshi, Taigo Horiguchi, Hideo Inoue, Intan Ruspita, Kaori Abe, Kikuji Yamashita, Eiji Hayashi and Takafumi Noma : Constitutive expression of thrombospondin 1 in MC3T3-E1 osteoblastic cells inhibits mineralization, Journal of Cellular Physiology, Vol.209, No.2, 322-332, 2006.
欧文冊子 ● Akemichi Ueno, Yoshihiro Miwa, Keiko Miyoshi, Taigo Horiguchi, Hideo Inoue, Intan Ruspita, Kaori Abe, Kikuji Yamashita, Eiji Hayashi and Takafumi Noma : Constitutive expression of thrombospondin 1 in MC3T3-E1 osteoblastic cells inhibits mineralization, Journal of Cellular Physiology, Vol.209, No.2, 322-332, 2006.

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