○種別 (必須): | □ | 学術論文 (審査論文)
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○言語 (必須): | □ | 英語
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○招待 (推奨): |
○審査 (推奨): |
○カテゴリ (推奨): |
○共著種別 (推奨): |
○学究種別 (推奨): |
○組織 (推奨): |
○著者 (必須): | 1. | (英) Sasagawa Takayo (日) (読)
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| 2. | (英) Oka Tatsuzo (日) (読)
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| 3. | 德村 彰
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| 4. | (英) Nishimoto Yuriko (日) (読)
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| 5. | (英) Munoz Saturnino (日) (読)
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| 6. | (英) Kuwahata Masashi (日) (読)
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| 7. | (英) Okita Misako (日) (読)
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| 8. | (英) Tsuji Hideaki (日) (読)
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| 9. | (英) Natori Yasuo (日) (読)
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○題名 (必須): | □ | (英) Analysis of the fatty acid components in a perchloric acid-soluble protein (日)
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○副題 (任意): |
○要約 (任意): | □ | (英) We had previously found that a perchloric acid-soluble protein (PSP1) occurs in rat liver, and that this novel protein inhibits protein synthesis in a rabbit reticulocyte lysate system (T. Oka, H. Tsuji, C. Noda, K. Sakai, Y.-H. Hong, I. Suzuki, S. Muñoz, Y. Natori, J. Biol. Chem. 270 (1995) 30060-30067). In the present study, we analyzed lipid components bound to PSP1. Native PSP1 was purified from rat liver using Sephadex G-75, DE-52 cellulose and IgGPSP-affinity chromatography, and the lipid components were extracted. The components obtained from the purified PSP1 were shown to be free fatty acids by thin-layer chromatography. By GC-MS, six major fatty acids were identified as 14:0, 16:0, 18:0, 18:1, 18:2 and 20:4. 1 mol of PSP1 contained 1.26 mol of total fatty acid components. The fatty acid-binding assay of PSP1 showed that the Bmax was 1.25 mol fatty acid/mol PSP1 and the Kd value for palmitic acid was 6.03 microM. The concentration of PSP1 mRNA in rat liver increased 2.3-fold by the administration of peroxisome proliferator, bezafibrate. These findings show that PSP1 is a fatty acid-binding protein-like protein, which is involved in the intracellular metabolism of fatty acid and is quite different from the known fatty acid-binding proteins. (日)
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○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Carrier Proteins (日) (読)
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| 3. | (英) Fatty Acid-Binding Proteins (日) (読)
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| 4. | (英) Fatty Acids (日) (読)
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| 5. | (英) Heat-Shock Proteins (日) (読)
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| 6. | (英) Liver (日) (読)
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| 7. | (英) Male (日) (読)
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| 8. | (英) Myelin P2 Protein (日) (読)
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| 9. | 心筋 (myocardium)
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| 10. | (英) Neoplasm Proteins (日) (読)
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| 11. | (英) Nerve Tissue Proteins (日) (読)
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| 12. | (英) Protein Synthesis Inhibitors (日) (読)
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| 13. | (英) RNA, Messenger (日) (読)
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| 14. | (英) Rats (日) (読)
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| 15. | (英) Rats, Wistar (日) (読)
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| 16. | (英) Recombinant Proteins (日) (読)
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| 17. | (英) Ribonucleases (日) (読)
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○発行所 (推奨): |
○誌名 (必須): | □ | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids ([Elsevier Science])
(pISSN: 1388-1981, eISSN: 1879-2618)
○ISSN (任意): | □ | 1388-1981
ISSN: 1388-1981
(pISSN: 1388-1981, eISSN: 1879-2618) Title: Biochimica et biophysica acta. Molecular and cell biology of lipidsTitle(ISO): Biochim Biophys Acta Mol Cell Biol LipidsPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login])
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○巻 (必須): | □ | 1437
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○号 (必須): | □ | 3
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○頁 (必須): | □ | 317 324
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○都市 (任意): |
○年月日 (必須): | □ | 西暦 1999年 3月 25日 (平成 11年 3月 25日)
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○URL (任意): | □ | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VNN-3W4GK5W-5&_coverDate=03%2F25%2F1999&_alid=436025813&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6183&_sort=d&view=c&_acct=C000008258&_version=1&_urlVersion=0&_userid=106892&md5=d5e49fe292242eb83201fcddb7e1dc68
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○DOI (任意): | □ | 10.1016/S1388-1981(99)00025-6 (→Scopusで検索)
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○PMID (任意): | □ | 10101265 (→Scopusで検索)
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○CRID (任意): |
○WOS (任意): | □ | 000079641400005
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○Scopus (任意): | □ | 2-s2.0-0033602463
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○評価値 (任意): |
○被引用数 (任意): |
○指導教員 (推奨): |
○備考 (任意): | 1. | (英) (日) 著者6.確認済 参照不要
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