『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Fujita Yoshiko (日) 藤田 佳子 (読) ふじた よしこ
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2.吉栖 正典
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3.井澤 有紀 ([徳島大学.大学院ヘルスバイオサイエンス研究部.神経情報医学部門.病態情報医学講座.薬理学])
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4. (英) Ali Nermin (日) アリ ネルミン (読) あり ねるみん
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5.兼松 康久 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.脳神経外科学]/[徳島大学.病院.中央診療施設等.地域脳神経外科診療部])
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6.石澤 啓介 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.臨床薬理学])
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7.土屋 浩一郎 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品機能生化学]/[徳島大学.医学部.医学科.病態情報医学講座])
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8.玉置 俊晃
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題名 (必須): (英) Transactivation of fetal liver kinase-1/kinase-insert domain-containing receptor by lysophosphatidylcholine induces vascular endothelial cell proliferation.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Lysophosphatidylcholine (LPC), a major lipid component of oxidized low-density lipoprotein, is a bioactive lipid molecule involved in numerous biological processes including the progression of atherosclerosis. Recently orphan G protein-coupled receptors were identified as high-affinity receptors for LPC. Although several G protein-coupled receptor ligands transactivate receptor tyrosine kinases, LPC-stimulated transactivation of receptor tyrosine kinase has not yet been reported. Here we observed for the first time that LPC treatment of human umbilical vein endothelial cells (HUVECs) induces tyrosyl phosphorylation of vascular endothelial growth factor receptor 2 [fetal liver kinase-1/kinase-insert domain-containing receptor, Flk-1/KDR)]. Flk-1/KDR transactivation by LPC was inhibited by vascular endothelial growth factor receptor tyrosine kinase inhibitors, SU1498 and 4-[(4'-chloro-2'-fluoro) phenylamino]6,7-dimethoxyquinazoline (VTKi) in immunoprecipitation. Furthermore, we examined the effects of the Src family kinases inhibitors, herbimycin A and 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo[3,4-d] pyrimidine (PP2), on LPC-induced Flk-1/KDR transactivation. Results from Western blots, c-Src is involved in LPC-induced Flk-1/KDR transactivation because herbimycin A and PP2 inhibited this transactivation. Kinase-inactive (KI) Src transfection also inhibited LPC-induced Flk-1/KDR transactivation. In addition, results from Western blots, ERK1/2 and Akt, which are downstream effectors of Flk-1/KDR, were also activated by LPC, and this was inhibited by SU1498, VTKi, herbimycin A, PP2, and KI Src transfection in HUVECs. LPC-induced stimulation of HUVEC proliferation was shown to be secondary to transactivation because it was suppressed by SU1498, VTKi, herbimycin A, PP2, and KI Src transfection in dimethylthiazoldiphenyltetra-zoliumbromide assay. These findings suggest that LPC-induced Flk-1/KDR transactivation via c-Src may have important implications for the progression of atherosclerosis.  (日)    [継承]
キーワード (推奨): 1.動脈硬化 (atherosclerosis) [継承]
2. (英) Benzoquinones (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) Cell Line (日) (読) [継承]
5. (英) Cell Proliferation (日) (読) [継承]
6. (英) Cell Survival (日) (読) [継承]
7. (英) Cells, Cultured (日) (読) [継承]
8. (英) Cinnamates (日) (読) [継承]
9. (英) Disease Progression (日) (読) [継承]
10. (英) Endothelial Cells (日) (読) [継承]
11. (英) Endothelium, Vascular (日) (読) [継承]
12. (英) Humans (日) (読) [継承]
13. (英) Immunoblotting (日) (読) [継承]
14. (英) Immunoprecipitation (日) (読) [継承]
15. (英) Lactams, Macrocyclic (日) (読) [継承]
16. (英) Ligands (日) (読) [継承]
17. (英) Lysophosphatidylcholines (日) (読) [継承]
18.リン酸化 (phosphorylation) [継承]
19. (英) Protein Structure, Tertiary (日) (読) [継承]
20. (英) Protein-Tyrosine Kinases (日) (読) [継承]
21. (英) Pyrimidines (日) (読) [継承]
22. (英) Quinazolines (日) (読) [継承]
23. (英) Quinones (日) (読) [継承]
24. (英) Transcriptional Activation (日) (読) [継承]
25. (英) Transfection (日) (読) [継承]
26. (英) Vascular Endothelial Growth Factor Receptor-2 (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Endocrinology ([The Endocrine Society])
(pISSN: 0013-7227, eISSN: 1945-7170)

ISSN (任意): 0013-7227
ISSN: 0013-7227 (pISSN: 0013-7227, eISSN: 1945-7170)
Title: Endocrinology
Title(ISO): Endocrinology
Publisher: Endocrine Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 147 [継承]
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年月日 (必須): 西暦 2006年 3月 初日 (平成 18年 3月 初日) [継承]
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DOI (任意): 10.1210/en.2005-0644    (→Scopusで検索) [継承]
PMID (任意): 16322069    (→Scopusで検索) [継承]
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WOS (任意): 000235350100039 [継承]
Scopus (任意): 2-s2.0-32644452665 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Pharmacology, The University of Tokushima Graduate School of Medical Sciences, 3-18-15 Kuramoto, Tokushima 770-8503, Japan. fujita56@ri.ncvc.go.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Yoshiko Fujita, Masanori Yoshizumi, Yuki Izawa, Nermin Ali, Yasuhisa Kanematsu, Keisuke Ishizawa, Koichiro Tsuchiya and Toshiaki Tamaki : Transactivation of fetal liver kinase-1/kinase-insert domain-containing receptor by lysophosphatidylcholine induces vascular endothelial cell proliferation., Endocrinology, Vol.147, No.3, 1377-1385, 2006.
欧文冊子 ● Yoshiko Fujita, Masanori Yoshizumi, Yuki Izawa, Nermin Ali, Yasuhisa Kanematsu, Keisuke Ishizawa, Koichiro Tsuchiya and Toshiaki Tamaki : Transactivation of fetal liver kinase-1/kinase-insert domain-containing receptor by lysophosphatidylcholine induces vascular endothelial cell proliferation., Endocrinology, Vol.147, No.3, 1377-1385, 2006.

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