○種別 (必須): | □ | 学術論文 (審査論文)
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○言語 (必須): | □ | 英語
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○著者 (必須): | 1. | (英) Sakurada Takumi (日) (読)
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| 2. | (英) Mima Kazuko (日) (読)
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| 3. | 栗崎 晃
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| 4. | 杉野 弘
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| 5. | 山内 卓
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○題名 (必須): | □ | (英) Neuronal cell type-specific promoter of the α CaM kinase II gene is activated by Zic2, a Zic family zinc finger protein (日)
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○要約 (任意): | □ | (英) To understand the neuronal cell type-specific expression of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II), we investigated binding proteins that specifically activated the promoter of the alpha isoform of CaM kinase II (alpha CaM kinase II). Proteins that bind the promoter sequence were found in rat brain nuclear extract by electrophoretic mobility shift assay. Then, we screened for binding proteins in a mouse brain cDNA library using the yeast one-hybrid system. Zic2, a Zic family zinc finger transcription factor, was identified as one of the binding proteins. To investigate the effect of Zic2 on the promoter activity, Zic2 cDNA was expressed with a luciferase reporter gene containing a neuronal cell type-specific promoter of alpha CaM kinase II in neuronal and non-neuronal cells. The promoter activity of alpha CaM kinase II was enhanced 1.3-5-fold in cultured neuronal cells by Zic2. The activation was varied among neuronal cell types. Zic2 also increased the promoter activity in non-neuronal cells, although the relative luciferase activites in non-neuronal cells were lower than those in neuronal cell lines. These results indicated that Zic2 was one of the proteins binding to, and regulating the activity of, the promoter of alpha CaM kinase II. (日)
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○キーワード (推奨): | 1. | (英) Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) (日) (読)
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| 2. | (英) Gene expression (日) (読)
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| 3. | (英) Neuronal cell (日) (読)
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| 4. | (英) Promoter-binding protein (日) (読)
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| 5. | (英) Yeast one-hybrid system (日) (読)
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| 6. | (英) Zic2 (日) (読)
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| 7. | (英) Zinc finger protein (日) (読)
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○発行所 (推奨): | □ | 日本神経科学学会
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○誌名 (必須): | □ | Neuroscience Research ([日本神経科学学会])
(pISSN: 0168-0102, eISSN: 1872-8111)
○ISSN (任意): | □ | 0168-0102
ISSN: 0168-0102
(pISSN: 0168-0102, eISSN: 1872-8111) Title: Neuroscience researchTitle(ISO): Neurosci ResPublisher: Elsevier Ireland Ltd (NLM Catalog)
(医中誌Web)
(Scopus)
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○巻 (必須): | □ | 53
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○号 (必須): | □ | 3
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○頁 (必須): | □ | 323 330
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○年月日 (必須): | □ | 西暦 2005年 11月 初日 (平成 17年 11月 初日)
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○URL (任意): | □ | http://dx.doi.org/10.1016/j.neures.2005.08.001
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○DOI (任意): | □ | 10.1016/j.neures.2005.08.001 (→Scopusで検索)
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○PMID (任意): | □ | 16157407 (→Scopusで検索)
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○備考 (任意): | 1. | (英) Article.Affiliation: Department of Biochemistry, Graduate School of Pharmaceutical Sciences, The University of Tokushima, Shomachi 1, Tokushima 770-8505, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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