『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=139265EID:139265, Map:0, LastModified:2012年10月12日(金) 17:04:02, Operator:[三木 ちひろ], Avail:TRUE, Censor:承認済, Owner:[[学科長]/[徳島大学.薬学部.薬学科]], Read:継承, Write:継承, Delete:継承.
○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
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○著者 (必須): 1. (英) Sogawa Yoshimi (日) (読)
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2.吉村 好之
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3.山内 卓
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○題名 (必須): □(英) Investigation of the Ca2+-independent form of Ca2+/calmodulin-dependent protein kinase II in neurite outgrowth  (日)    [継承]
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○要約 (任意): □(英) Neuronal Ca(2+)/calmodulin-dependent protein kinase II (CaM kinase II) plays important roles in the control of nerve functions in response to intracellular Ca(2+) (for reviews [Annu. Rev. Physiol. 57 (1995) 417-445; Trends Neurosci. 17 (1994) 406-412]). Brief Ca(2+) signals activate CaM kinase II, and stimulate an autophosphorylation of Thr-286 which allows the kinase to maintain its activated state even after the Ca(2+) concentration has returned to basal levels [J. Biol. Chem. 264 (1989) 16759-16763; Neuron 3 (1989) 59-70; J. Biochem. 109 (1991) 137-143]. Autophosphorylation of CaM kinase II occurs in situ, but it occurs relatively quickly, within just a few minutes [Endocrinology 134 (1994) 2245-2250; J. Biol. Chem. 268 (1993) 7863-7867; J. Biol. Chem. 265 (1990) 18055-18058]. In the present study, we investigated the involvement of the autophosphorylated/Ca(2+)-independent form of CaM kinase II in neurite outgrowth. When neuroblastoma Neruo2a (Nb2a) cells expressing the alpha isoform of CaM kinase II (Nb2a/alpha cells) were stimulated by plating, they formed neurites. The autophosphorylation of Thr-286 and appearance of Ca(2+)-independent activity preceded the neurite formation. The effect of mutating of the kinase autophosphorylation site replacing Thr-286 with Ala (alpha T286A kinase) or Asp (alpha T286D kinase) was examined. alpha T286A kinase was not converted to a Ca(2+)-independent form, and alpha T286D kinase had Ca(2+)-independent activity significantly as an autophosphorylated kinase. Cells expressing alpha T286D kinase had much longer neurites than Nb2a/alpha cells, whereas cells with alpha T286A kinase did not form neurites. These results indicated that the Ca(2+)-independent form of CaM kinase II autophosphorylated at Thr-286 is involved in neurite outgrowth.  (日)    [継承]
○キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Calcium (日) (読) [継承]
3. (英) Calcium-Calmodulin-Dependent Protein Kinase Type 2 (日) (読) [継承]
4. (英) Calcium-Calmodulin-Dependent Protein Kinases (日) (読) [継承]
5. (英) Cell Line (日) (読) [継承]
6. (英) DNA, Complementary (日) (読) [継承]
7. (英) Fluorescent Dyes (日) (読) [継承]
8. (英) Green Fluorescent Proteins (日) (読) [継承]
9. (英) Immunoblotting (日) (読) [継承]
10. (英) Indicators and Reagents (日) (読) [継承]
11. (英) Isoenzymes (日) (読) [継承]
12. (英) Luminescent Proteins (日) (読) [継承]
13. (英) Mice (日) (読) [継承]
14. (英) Microscopy, Fluorescence (日) (読) [継承]
15. (英) Mutation (日) (読) [継承]
16. (英) Neurites (日) (読) [継承]
17. (英) Neuroblastoma (日) (読) [継承]
18.リン酸化 (phosphorylation) [継承]
19. (英) Transfection (日) (読) [継承]
○発行所 (推奨): □Elsevier Science [継承]
○誌名 (必須): □Brain Research. Brain Research Protocols ([Elsevier])
(pISSN: 1385-299X)

○ISSN (任意): □1385-299X
ISSN: 1385-299X (pISSN: 1385-299X)
Title: Brain research. Brain research protocols
Title(ISO): Brain Res Brain Res Protoc
Publisher: Elsevier B.V.
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○巻 (必須): □8 [継承]
○号 (必須): □3 [継承]
○頁 (必須): □159 169 [継承]
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○年月日 (必須): □西暦 2001年 12月 初日 (平成 13年 12月 初日) [継承]
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○DOI (任意): □10.1016/S1385-299X(01)00106-4    (→Scopusで検索) [継承]
○PMID (任意): □11733191    (→Scopusで検索) [継承]
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○備考 (任意): 1.(英) Article.Affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Shomachi 1, Tokushima 770-8505, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yoshimi Sogawa, Yoshiyuki Yoshimura and Takashi Yamauchi : Investigation of the Ca2+-independent form of Ca2+/calmodulin-dependent protein kinase II in neurite outgrowth, Brain Research. Brain Research Protocols, 8, 3, 159-169, 2001.
欧文冊子 ● Yoshimi Sogawa, Yoshiyuki Yoshimura and Takashi Yamauchi : Investigation of the Ca2+-independent form of Ca2+/calmodulin-dependent protein kinase II in neurite outgrowth, Brain Research. Brain Research Protocols, 8, 3, 159-169, 2001.

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