| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Urushihara Masato (日) (読)
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| 2. | 山内 卓
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| ○題名 (必須): | □ | (英) Role of β isoform-specific insertions of Ca2+/calmodulin-dependent protein kinase II (日)
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| ○要約 (任意): | □ | (英) Alpha and beta isoforms of Ca2+/calmodulin-dependent protein kinase II (alpha and beta CaM kinase II, respectively) are highly conserved except for beta-specific insertions 1 and 2, located at amino acids 316-340 and 354-392, respectively. To investigate the role of these beta-specific insertions, we prepared the deletion mutants betaDelta1, betaDelta2 and betaDelta1/2, which lacked insertions 1, 2 and both, respectively. These mutant DNAs were expressed in neuroblastoma cells and compared with the wild-type enzyme. Green fluorescent protein tagged CaM kinase II was used to further explore the distribution of the kinase in living cells. Most (80%) of wild-type beta and mutant betaDelta1 were located in the particulate fraction, and distributed in the cell body and neurites, forming punctate or spot-like structures in the neurites. Mutants betaDelta2 and betaDelta1/2 were distributed in almost equal amounts in the soluble and particulate fractions. They were concentrated in the base of neurites and only partlially distributed throughout neurites, indicating that their transport to neurites was impaired. Beta(1-410), a deletion mutant of the association domain with a monomeric form, was located primarily in the soluble fraction. These results indicate that insertion 2, the association domain, and the oligomeric form of beta CaM kinase II play an important role in the cellular distribution of beta CaM kinase II. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Calcium (日) (読)
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| 3. | (英) Calcium-Calmodulin-Dependent Protein Kinase Type 2 (日) (読)
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| 4. | (英) Calcium-Calmodulin-Dependent Protein Kinases (日) (読)
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| 5. | (英) Calmodulin (日) (読)
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| 6. | (英) Green Fluorescent Proteins (日) (読)
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| 7. | (英) Isoenzymes (日) (読)
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| 8. | (英) Luminescent Proteins (日) (読)
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| 9. | (英) Mice (日) (読)
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| 10. | (英) Mutation (日) (読)
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| 11. | (英) Tumor Cells, Cultured (日) (読)
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| ○発行所 (推奨): | □ | Federation of European Biochemical Societies
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| ○誌名 (必須): | □ | European Journal of Biochemistry ([Federation of European Biochemical Societies])
(pISSN: 0014-2956, eISSN: 1432-1033)
| ○ISSN (任意): | □ | 0014-2956
ISSN: 0014-2956
(pISSN: 0014-2956, eISSN: 1432-1033) Title: European journal of biochemistryTitle(ISO): Eur J BiochemPublisher: Wiley-Blackwell Publishing Ltd (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 268
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| ○号 (必須): | □ | 17
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| ○頁 (必須): | □ | 4802 4808
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2001年 9月 初日 (平成 13年 9月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1046/j.1432-1327.2001.02410.x (→Scopusで検索)
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| ○PMID (任意): | □ | 11532017 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-0035723727
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| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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