| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | 吉村 好之
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| 2. | (英) Aoi Chiaki (日) (読)
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| 3. | 山内 卓
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| ○題名 (必須): | □ | (英) Investigation of protein substrates of Ca2+/calmodulin-dependent protein kinase II translocated to the postsynaptic density (日)
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| ○要約 (任意): | □ | (英) To elucidate the physiological significance of the translocation of Ca(2+)/calmodulin-dependent protein kinase II (CaM kinase II), we investigated substrates of CaM kinase II in the postsynaptic density (PSD). PSD proteins were phosphorylated by CaM kinase II of its PSD complex, and separated by two-dimensional gel electrophoresis. More than 28 proteins were phosphorylated under experimental conditions. Proteins corresponding to CaM kinase II substrates were excised from the gels, eluted electrophoretically, and then sequenced. Several substrates were identified, including PSD95, SAP90, alpha-internexin, neurofilament L chain, cAMP phosphodiesterase, and alpha- and beta-tubulin. Some substrates were also identified by immunoblotting, including N-methyl-D-aspartic acid (NMDA) receptor 2B subunit, 1-alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor 1 (GluR1), neurofilament H chain and dynamin. PSD95, SAP90, dynamin, and alpha-internexin were demonstrated for the first time to be substrates of CaM kinase II. NMDA receptor 2B subunit and GluR1 existed as major substrates in the PSD. Moreover, translocation of CaM kinase II was inhibited by phosphorylation of PSD proteins. These results suggest that CaM kinase II plays important roles in the regulation of synaptic functions through phosphorylation of PSD proteins. (日)
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| ○キーワード (推奨): | 1. | (英) Ca2+/calmodulin-dependent protein kinase II (日) (読)
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| 2. | (英) Postsynaptic density (日) (読)
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| 3. | リン酸化 (phosphorylation)
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| 4. | (英) Translocation (日) (読)
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| 5. | (英) Two-dimensional gel electrophoresis (日) (読)
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| ○発行所 (推奨): | □ | Elsevier Science
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| ○誌名 (必須): | □ | Brain Research. Molecular Brain Research (Elsevier)
(pISSN: 0169-328X)
| ○ISSN (任意): | □ | 0169-328X
ISSN: 0169-328X
(pISSN: 0169-328X) Title: Brain research. Molecular brain researchTitle(ISO): Brain Res Mol Brain ResPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 81
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| ○号 (必須): | □ | 1-2
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| ○頁 (必須): | □ | 118 128
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2000年 9月 30日 (平成 12年 9月 30日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/S0169-328X(00)00170-4 (→Scopusで検索)
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| ○PMID (任意): | □ | 11000484 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Shomachi 1, Tokushima 770-8505, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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