『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=137767)

EID=137767EID:137767, Map:0, LastModified:2013年5月1日(水) 17:29:29, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[島田 光生], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Harada Noboru (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.島田 光生 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Okano Shinji (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Suehiro Taketoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.副島 雄二
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Tomita Yukihiro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Maehara Yoshihiko (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) IL-12 gene therapy is an effective therapeutic strategy for hepatocellular carcinoma in immunosuppressed mice  (日)    [継承]
副題 (任意):
要約 (任意): (英) Immunosuppressive therapy for organ transplantation is essential for controlling rejection. When liver transplantation is performed as a therapy for hepatocellular carcinoma (HCC), recurrent HCC is one of the most fatal complications. In this study, we show that intratumoral murine IL-12 (mIL-12) gene therapy has the potential to be an effective treatment for malignancies under immunosuppression. C3H mice (H-2(k)), injected with FK506 (3 mg/kg) i.p., were s.c. implanted with 2.5 x 10(6) MH134 cells (H-2(k)) and we treated the established HCC with electroporation-mediated gene therapy using mIL-12 plasmid DNA. Intratumoral gene transfer of mIL-12 elevated intratumoral mIL-12, IFN-gamma, and IFN-gamma-inducible protein-10, significantly reduced the number of microvessels and inhibited the growth of HCC, compared with HCC-transferred control pCAGGS plasmid. The inhibition of tumor growth in immunosuppressed mice was comparable with that of mIL-12 gene therapy in immunocompetent mice. Intratumoral mIL-12 gene therapy enhanced lymphocytic infiltration into the tumor and elicited the MH134-specific CTL response even under FK506. The dose of FK506 was sufficient to prevent the rejection of distant allogenic skin grafts (BALB/c mice, H-2(d)) and tumors, B7-p815 (H-2(d)) used as transplants, during mIL-12 gene therapy against MH134. Ab-mediated depletion studies suggested that the inhibition of tumor growth, neovascularization, and spontaneous lung metastasis by mIL-12 was dependent almost entirely on NK cells and partially on T cells. These results suggest that intratumoral mIL-12 gene therapy is a potent effective strategy not only to treat recurrences of HCC in liver transplantation, but also to treat solid malignant tumors in immunosuppressed patients with transplanted organ.  (日)    [継承]
キーワード (推奨): 1. (英) Angiogenesis Inhibitors (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Antineoplastic Agents (日) (読) [継承]
4. (英) Carcinoma, Hepatocellular (日) (読) [継承]
5. (英) Cell Line, Tumor (日) (読) [継承]
6. (英) Dose-Response Relationship, Immunologic (日) (読) [継承]
7. (英) Electroporation (日) (読) [継承]
8. (英) Female (日) (読) [継承]
9. (英) Gene Transfer Techniques (日) (読) [継承]
10. (英) Genetic Therapy (日) (読) [継承]
11. (英) Genetic Vectors (日) (読) [継承]
12. (英) Graft Rejection (日) (読) [継承]
13. (英) Growth Inhibitors (日) (読) [継承]
14. (英) Immunosuppressive Agents (日) (読) [継承]
15. (英) Interleukin-12 (日) (読) [継承]
16. (英) Killer Cells, Natural (日) (読) [継承]
17. (英) Liver Neoplasms, Experimental (日) (読) [継承]
18. (英) Mice (日) (読) [継承]
19. (英) Mice, Inbred BALB C (日) (読) [継承]
20. (英) Mice, Inbred C3H (日) (読) [継承]
21. (英) Neoplasm Metastasis (日) (読) [継承]
22. (英) Neoplasm Transplantation (日) (読) [継承]
23. (英) Skin Transplantation (日) (読) [継承]
24. (英) T-Lymphocyte Subsets (日) (読) [継承]
25. (英) T-Lymphocytes, Cytotoxic (日) (読) [継承]
26. (英) Tacrolimus (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Immunology ([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)

ISSN (任意): 0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 173 [継承]
(必須): 11 [継承]
(必須): 6635 6644 [継承]
都市 (任意):
年月日 (必須): 西暦 2004年 12月 1日 (平成 16年 12月 1日) [継承]
URL (任意): http://www.jimmunol.org/cgi/content/abstract/173/11/6635 [継承]
DOI (任意):
PMID (任意): 15557154    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000225307500018 [継承]
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.Affiliation: Department of, Surgery and Science, Department of Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. nharada@surg2.med.kyushu-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Noboru Harada, Mitsuo Shimada, Shinji Okano, Taketoshi Suehiro, Yuji Soejima, Yukihiro Tomita and Yoshihiko Maehara : IL-12 gene therapy is an effective therapeutic strategy for hepatocellular carcinoma in immunosuppressed mice, The Journal of Immunology, Vol.173, No.11, 6635-6644, 2004.
欧文冊子 ● Noboru Harada, Mitsuo Shimada, Shinji Okano, Taketoshi Suehiro, Yuji Soejima, Yukihiro Tomita and Yoshihiko Maehara : IL-12 gene therapy is an effective therapeutic strategy for hepatocellular carcinoma in immunosuppressed mice, The Journal of Immunology, Vol.173, No.11, 6635-6644, 2004.

関連情報

Number of session users = 1, LA = 1.62, Max(EID) = 383267, Max(EOID) = 1024361.