『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=137497)

EID=137497EID:137497, Map:0, LastModified:2012年11月3日(土) 17:46:09, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[辻 明彦], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.工学部.生物工学科.生物反応工学講座 [継承]
著者 (必須): 1.辻 明彦
役割 (任意): (英)   (日) 計画,実験,解析,考察,論文執筆   [継承]
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学籍番号 (推奨):
[継承]
2. (英) Kikuchi Yayoi (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Sato Yukimi (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Koide Shizuyo (日) (読)
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学籍番号 (推奨):
[継承]
5.湯浅 恵造 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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学籍番号 (推奨):
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6.長浜 正巳
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学籍番号 (推奨):
[継承]
7.松田 佳子
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学籍番号 (推奨):
[継承]
題名 (必須): (英) A proteomic approach reveals transient association of reticulocalbin-3, a novel member of the CREC family, with the precursor of subtilisin-like proprotein convertase, PACE4  (日)    [継承]
副題 (任意):
要約 (任意): (英) SPCs (subtilisin-like proprotein convertases) are a family of seven structurally related serine endoproteases that are involved in the proteolytic activation of proproteins. In an effort to examine the substrate protein for PACE4 (paired basic amino-acid-cleaving enzyme-4), an SPC, a potent protein inhibitor of PACE4, an alpha1-antitrypsin RVRR (Arg-Val-Arg-Arg) variant, was expressed in GH4C1 cells. Ectopic expression of the RVRR variant caused accumulation of the 48 kDa protein in cells. Sequence analysis indicates that the 48 kDa protein is a putative Ca2+-binding protein, RCN-3 (reticulocalbin-3), which had previously been predicted by bioinformatic analysis of cDNA from the human hypothalamus. RCN-3 is a member of the CREC (Cab45/reticulocalbin/ERC45/calumenin) family of multiple EF-hand Ca2+-binding proteins localized to the secretory pathway. The most interesting feature of the RCN-3 sequence is the presence of five Arg-Xaa-Xaa-Arg motifs, which represents the target sequence of SPCs. Biosynthetic studies showed that RCN-3 is transiently associated with proPACE4, but not with mature PACE4. Inhibition of PACE4 maturation by a Ca2+ ionophore resulted in accumulation of the proPACE4-RCN-3 complex in cells. Furthermore, autoactivation and secretion of PACE4 was increased upon co-expression with RCN-3. Our findings suggest that selective and transient association of RCN-3 with the precursor of PACE4 plays an important role in the biosynthesis of PACE4.  (日)    [継承]
キーワード (推奨): 1. (英) Adenoma (日) (読) [継承]
2. (英) Amino Acid Sequence (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) COS Cells (日) (読) [継承]
5. (英) Calcium-Binding Proteins (日) (読) [継承]
6. (英) Cell Line (日) (読) [継承]
7. (英) Cell Line, Tumor (日) (読) [継承]
8. (英) Cercopithecus aethiops (日) (読) [継承]
9. (英) Enzyme Activation (日) (読) [継承]
10. (英) Furin (日) (読) [継承]
11. (英) Humans (日) (読) [継承]
12. (英) Kidney (日) (読) [継承]
13. (英) Molecular Sequence Data (日) (読) [継承]
14. (英) Neoplasm Proteins (日) (読) [継承]
15. (英) Pituitary Neoplasms (日) (読) [継承]
16. (英) Proprotein Convertase 5 (日) (読) [継承]
17. (英) Proprotein Convertases (日) (読) [継承]
18. (英) Protein Binding (日) (読) [継承]
19. (英) Protein Precursors (日) (読) [継承]
20. (英) Proteomics (日) (読) [継承]
21. (英) Rats (日) (読) [継承]
22. (英) Recombinant Fusion Proteins (日) (読) [継承]
23. (英) Sequence Alignment (日) (読) [継承]
24. (英) Sequence Homology, Amino Acid (日) (読) [継承]
25. (英) Serine Endopeptidases (日) (読) [継承]
26. (英) Transfection (日) (読) [継承]
27. (英) alpha 1-Antitrypsin (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 1470-8728
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2006年 4月 初日 (平成 18年 4月 初日) [継承]
URL (任意):
DOI (任意): 10.1042/BJ20051524    (→Scopusで検索) [継承]
PMID (任意): 16433634    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, 2-1 Minamijosanjima, Tokushima, 770-8506, Japan. tsuji@bio.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Comparative Study  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) OtherID: PMC1449992  (日)    [継承]

標準的な表示

和文冊子 ● Akihiko Tsuji, Yayoi Kikuchi, Yukimi Sato, Shizuyo Koide, Keizo Yuasa, Masami Nagahama and Yoshiko Matsuda : A proteomic approach reveals transient association of reticulocalbin-3, a novel member of the CREC family, with the precursor of subtilisin-like proprotein convertase, PACE4, The Biochemical Journal, Vol.396, No.1, 51-59, 2006.
欧文冊子 ● Akihiko Tsuji, Yayoi Kikuchi, Yukimi Sato, Shizuyo Koide, Keizo Yuasa, Masami Nagahama and Yoshiko Matsuda : A proteomic approach reveals transient association of reticulocalbin-3, a novel member of the CREC family, with the precursor of subtilisin-like proprotein convertase, PACE4, The Biochemical Journal, Vol.396, No.1, 51-59, 2006.

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