『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=136739EID:136739, Map:0, LastModified:2012年12月21日(金) 11:09:39, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[副研究部長]/[徳島大学.大学院ヘルスバイオサイエンス研究部]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1. (英) Nakanishi Shusuke (日) (読)
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2.桑原 知巳
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3.中山 治之
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4. (英) Tanaka Mamoru (日) (読)
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5. (英) Ohnishi Yoshinari (日) (読)
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題名 (必須): (英) Rapid Species Identification and Partial Strain Differentiation of Clostridium butyricum by PCR Using 16S-23S rDNA Intergenic Spacer Regions  (日)    [継承]
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要約 (任意): (英) Some Clostridium butyricum strains have been used as probiotics for both humans and animals. Strain-specific identification is necessary for the manufacturing process of probiotics. The aim of this study was to determine whether there are sufficient genetic variations in 16S-23S intergenic spacer regions (ISRs) to discriminate C. butyricum at the biovar level. We amplified ISRs from five reference strains, a probiotic strain (MIYAIRI 588) and 22 isolates, and we classified them into four groups on the basis of amplification patterns (type A through D). However, amplification of ISRs is not sufficient for discriminating strains. Moreover, we compared genetic structures of these ISRs. Sequence analysis revealed that the size variations of ISRs were generated by the insertion of tRNA genes and unique sequences into the internal portion, while the external portions were highly conserved. On the basis of the highly conserved nucleotide sequences within the ISRs, we developed a PCR primer set specific to C. butyricum. In addition, the PCR primer designed from the unique inserted sequence in type B strain was useful to differentiate probiotic strains at the biovar level.  (日)    [継承]
キーワード (推奨): 1. (英) Clostridium butyricum (日) (読) [継承]
2. (英) 16S-23S rDNA intergenic spacer regions (日) (読) [継承]
3. (英) PCR (日) (読) [継承]
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誌名 (必須): Microbiology and Immunology ([日本細菌学会])
(pISSN: 0385-5600, eISSN: 1348-0421)

ISSN (任意): 0385-5600
ISSN: 0385-5600 (pISSN: 0385-5600, eISSN: 1348-0421)
Title: Microbiology and immunology
Title(ISO): Microbiol Immunol
Supplier: 財団法人 学会誌刊行センター
Publisher: Wiley Publishing
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年月日 (必須): 西暦 2005年 0月 初日 (平成 17年 0月 初日) [継承]
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PMID (任意): 16034204    (→Scopusで検索) [継承]
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WOS (任意): 000230410300006 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan. s.nakanishi@miyarisan.com  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

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和文冊子 ● Shusuke Nakanishi, Tomomi Kuwahara, Haruyuki Nakayama, Mamoru Tanaka and Yoshinari Ohnishi : Rapid Species Identification and Partial Strain Differentiation of Clostridium butyricum by PCR Using 16S-23S rDNA Intergenic Spacer Regions, Microbiology and Immunology, Vol.49, No.7, 613-621, 2005.
欧文冊子 ● Shusuke Nakanishi, Tomomi Kuwahara, Haruyuki Nakayama, Mamoru Tanaka and Yoshinari Ohnishi : Rapid Species Identification and Partial Strain Differentiation of Clostridium butyricum by PCR Using 16S-23S rDNA Intergenic Spacer Regions, Microbiology and Immunology, Vol.49, No.7, 613-621, 2005.

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