『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1.前川 正彦
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2. (英) Kamada Masaharu (日) (読)
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3.山本 哲史
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4.山野 修司
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5.苛原 稔
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6.木戸 博 ([徳島大学.先端酵素学研究所.重点研究部門])
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7.青野 敏博
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題名 (必須): (英) Involvement of carbohydrate molecules on zona pellucida in human fertilization  (日)    [継承]
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要約 (任意): (英) Although the involvement of several receptors and ligand molecules in sperm-zona interaction in many species have been proposed, there has been a little analysis of the kinetics between these molecules during the interaction. In the present study, we applied the detection method using surface plasmon resonance (SPR) by a BIAcore apparatus for the analysis of the putative receptor-ligand interaction of sperm-egg binding. Mannose-BSA or [man](5)-[GlcNAc](2)-Asp was immobilized on the surface of a sensor chip. When concanavalin A (Con A) was delivered to each of two different sensor chips to evaluate their usefulness, the resonance signal after sample injection onto a [man](5)-[GlcNAc](2)-Asp-fixed chip decreased rapidly than the mannose-BSA-fixed chip. However, the amount of binding for Con A during the injection onto the [man](5)-[GlcNAc](2)-Asp-fixed chip was high. When acid sperm extracts (acid extracts) and fractions through a CM column, containing protease activity (protease fractions), were delivered to the mannose-BSA-fixed chip, the SPR signal during the injection was not obviously changed compared with that of the control. However, when sperm samples were delivered to the [man](5)-[GlcNAc](2)-Asp-fixed chip, the SPR response during the injection was enormous. These results suggest that the [man](5)-[GlcNAc](2)-Asp-fixed chip is more useful than the mannose-BSA-fixed chip for investigating the interactions with sperm extracts and that the sensitive method using SPR by a BIAcore apparatus is applicable for the analysis of the putative receptor-ligand interaction of sperm-egg binding.  (日)    [継承]
キーワード (推奨): 1. (英) Mannose (日) (読) [継承]
2. (英) Surface plasmon resonance (日) (読) [継承]
3. (英) Sperm-Zona interaction (日) (読) [継承]
4. (英) Lectin-carbohydrate binding (日) (読) [継承]
発行所 (推奨): Elsevier (->組織[Elsevier Science]) [継承]
誌名 (必須): Journal of Reproductive Immunology (European Society of Reproductive Immunology)
(pISSN: 0165-0378, eISSN: 1872-7603)

ISSN (任意): 0165-0378
ISSN: 0165-0378 (pISSN: 0165-0378, eISSN: 1872-7603)
Title: Journal of reproductive immunology
Title(ISO): J Reprod Immunol
Publisher: Elsevier Ireland Ltd
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(必須): 79 89 [継承]
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年月日 (必須): 西暦 2002年 1月 初日 (平成 14年 1月 初日) [継承]
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DOI (任意): 10.1016/S0165-0378(01)00096-1    (→Scopusで検索) [継承]
PMID (任意): 11730906    (→Scopusで検索) [継承]
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WOS (任意): 000172873300008 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan. masahiko@clin.med.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: In Vitro  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Masahiko Maegawa, Masaharu Kamada, Satoshi Yamamoto, Shuji Yamano, Minoru Irahara, Hiroshi Kido and Toshihiro Aono : Involvement of carbohydrate molecules on zona pellucida in human fertilization, Journal of Reproductive Immunology, Vol.53, No.1-2, 79-89, 2002.
欧文冊子 ● Masahiko Maegawa, Masaharu Kamada, Satoshi Yamamoto, Shuji Yamano, Minoru Irahara, Hiroshi Kido and Toshihiro Aono : Involvement of carbohydrate molecules on zona pellucida in human fertilization, Journal of Reproductive Immunology, Vol.53, No.1-2, 79-89, 2002.

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