『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=135826EID:135826, Map:0, LastModified:2016年7月25日(月) 15:44:42, Operator:[三好 小文], Avail:TRUE, Censor:0, Owner:[野間 隆文], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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共著種別 (推奨): 徳島大学以外での国内共著 (徳島大学以外でのある一つの国に属する複数機関による共同研究 (国外研究者を含まない)) [継承]
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組織 (推奨):
著者 (必須): 1. (英) Yoshimura Kiyoshi (日) (読)
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2. (英) Hazama Shoichi (日) (読)
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3. (英) Iizuka Norio (日) (読)
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4. (英) Yoshino Shigefumi (日) (読)
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5. (英) Yamamoto Koutaro (日) (読)
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6. (英) Muraguchi Masahiro (日) (読)
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7. (英) Ohmoto Yasuichi (日) (読)
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8.野間 隆文 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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9. (英) Oka Masaaki (日) (読)
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題名 (必須): (英) Successful immunogene therapy using colon cancer cells (colon 26) transfected with plasmid vector containing mature interleukin-18 cDNA and the Ig leader sequence  (日)    [継承]
副題 (任意):
要約 (任意): (英) IL-18 is a novel cytokine that induces interferon (IFN)-gamma secretion and plays an important role in antitumor immunity. In the present study, we constructed plasmid vectors encoding the murine mature IL-18 cDNA linked with the Igkappa leader sequence and the pro-IL-18 cDNA to estimate the efficacy of the mature IL- 18 vector and to evaluate IL-18--producing tumor cells as a tumor vaccine. Colon 26 cells were transfected with the abovementioned vectors or with vector alone (mock). Reverse transcription-polymerase chain reaction analysis showed increased expression of murine IL-18 cDNA in both mature IL-18 and pro-IL-18 transfectants in comparison to that in mock transfected cells. The ability of the culture supernatants of mature IL-18 transfectants to induce IFN-gamma secretion was extremely high (40-140 pg/10(6) cells) in comparison to that of pro-IL-18 transfectants (4-18 pg/10(6) cells). When injected into syngeneic BALB/c mice, the growth of mature IL-18 transfectants, but not pro-IL-18 transfectants, was significantly less than that in mock transfected cells ( P< .01, by ANOVA and analysis of covariance). In addition, injection of colon 26 or Meth-A cells into mice immunized with a mature IL-18 transfectant revealed acquired immunity. Depletion of natural killer cells did not affect the growth of transfectants. However, the growth inhibitory effects were partially abrogated following treatment with anti-CD4+ and anti-CD8+ antibodies. These data suggest that the rejection of mature IL-18/colon 26 cells was mediated through T-cell activation. Gene therapy using mature IL-18 transfectants containing a plasmid vector and the Igkappa leader sequence may be a useful tumor vaccine.  (日)    [継承]
キーワード (推奨): 1. (英) IL-18 (日) (読) [継承]
2. (英) gene therapy (日) (読) [継承]
3. (英) plasmid (日) (読) [継承]
4. (英) immunotherapy (日) (読) [継承]
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誌名 (必須): Cancer Gene Therapy ([Nature Publishing Group])
(pISSN: 0929-1903, eISSN: 1476-5500)

ISSN (任意): 0929-1903
ISSN: 0929-1903 (pISSN: 0929-1903, eISSN: 1476-5500)
Title: Cancer gene therapy
Title(ISO): Cancer Gene Ther.
Publisher: Nature Publishing Group
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2001年 1月 8日 (平成 13年 1月 8日) [継承]
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DOI (任意): 10.1038/sj.cgt.7700277    (→Scopusで検索) [継承]
PMID (任意): 11219497    (→Scopusで検索) [継承]
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WOS (任意): 000166879400002 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Surgery II, Yamaguchi University School of Medicine, Ube, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Kiyoshi Yoshimura, Shoichi Hazama, Norio Iizuka, Shigefumi Yoshino, Koutaro Yamamoto, Masahiro Muraguchi, Yasuichi Ohmoto, Takafumi Noma and Masaaki Oka : Successful immunogene therapy using colon cancer cells (colon 26) transfected with plasmid vector containing mature interleukin-18 cDNA and the Ig leader sequence, Cancer Gene Therapy, Vol.8, No.1, 9-16, 2001.
欧文冊子 ● Kiyoshi Yoshimura, Shoichi Hazama, Norio Iizuka, Shigefumi Yoshino, Koutaro Yamamoto, Masahiro Muraguchi, Yasuichi Ohmoto, Takafumi Noma and Masaaki Oka : Successful immunogene therapy using colon cancer cells (colon 26) transfected with plasmid vector containing mature interleukin-18 cDNA and the Ig leader sequence, Cancer Gene Therapy, Vol.8, No.1, 9-16, 2001.

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