『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=135818EID:135818, Map:0, LastModified:2016年11月22日(火) 14:13:11, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[野間 隆文], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Mori N (日) (読)
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学籍番号 (推奨):
[継承]
2. (英) Oka M (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Hazama S (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Iizuka N (日) (読)
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学籍番号 (推奨):
[継承]
5. (英) Yamamoto K (日) (読)
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学籍番号 (推奨):
[継承]
6. (英) Yoshino S (日) (読)
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[継承]
7.丹黒 章 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.胸部・内分泌・腫瘍外科学])
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学籍番号 (推奨):
[継承]
8.野間 隆文 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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学籍番号 (推奨):
[継承]
9. (英) Hirose K (日) (読)
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[継承]
題名 (必須): (英) Detection of telomerase activity in peritoneal lavage fluid from patients with gastric cancer using immunomagnetic beads  (日)    [継承]
副題 (任意):
要約 (任意): (英) Cytologic examination of peritoneal lavage fluid is a useful predictor of peritoneal recurrence in gastric cancer. However, this technique is not overly sensitive and requires special abilities in the cytologist. In this study, telomerase activity was used to detect free cancer cells in peritoneal lavage fluid from patients with gastric cancer. In the first part, 12 lavage-fluid samples obtained from 12 patients with gastric cancer were analysed using the conventional telomeric repeat amplification protocol (TRAP) assay. Three of five patients with early gastric cancer had positive telomerase activity. These false-positive results may have been due to lymphocyte contamination. Furthermore, polymerase chain reaction inhibitors were also detected in the lavage-fluid samples. Therefore, we developed a novel method for elimination of haematopoietic cell and Taq polymerase inhibitors to increase the accuracy of the TRAP assay using immunomagnetic beads, which bind to most normal and neoplastic human epithelial cells. Telomerase activity was found in 10 of 20 (50%) lavage-fluid samples from patients with serosal or subserosal invasion. Cytologic examination was positive in nine of 20 (45%) samples. Both the telomerase activity and cytology were negative in all 14 patients without serosal or subserosal invasion. These results suggest that the TRAP assay combined with immunomagnetic beads might be useful for detection of free cancer cells in the peritoneal space in gastric cancer without the aid of an experienced cytologist.  (日)    [継承]
キーワード (推奨): 1. (英) Adenocarcinoma (日) (読) [継承]
2. (英) Adult (日) (読) [継承]
3. (英) Aged (日) (読) [継承]
4. (英) Ascitic Fluid (日) (読) [継承]
5. (英) False Positive Reactions (日) (読) [継承]
6. (英) Female (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Immunomagnetic Separation (日) (読) [継承]
9. (英) Male (日) (読) [継承]
10. (英) Middle Aged (日) (読) [継承]
11. (英) Peritoneal Lavage (日) (読) [継承]
12. (英) Polymerase Chain Reaction (日) (読) [継承]
13. (英) Reproducibility of Results (日) (読) [継承]
14. (英) Stomach Neoplasms (日) (読) [継承]
15. (英) Telomerase (日) (読) [継承]
16. (英) Tumor Cells, Cultured (日) (読) [継承]
発行所 (推奨):
誌名 (必須): British Journal of Cancer (British Empire Cancer Campaign/Cancer Research UK)
(pISSN: 0007-0920, eISSN: 1532-1827)

ISSN (任意): 0007-0920
ISSN: 0007-0920 (pISSN: 0007-0920, eISSN: 1532-1827)
Title: British journal of cancer
Title(ISO): Br. J. Cancer
Publisher: Nature Publishing Group
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 83 [継承]
(必須): 8 [継承]
(必須): 1026 1032 [継承]
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年月日 (必須): 西暦 2000年 10月 初日 (平成 12年 10月 初日) [継承]
URL (任意): http://www.nature.com/bjc/journal/v83/n8/abs/6691408a.html [継承]
DOI (任意): 10.1054/bjoc.2000.1408    (→Scopusで検索) [継承]
PMID (任意): 10993650    (→Scopusで検索) [継承]
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WOS (任意): 000089733300010 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
3.(英) OtherID: PMC2363555  (日)    [継承]

標準的な表示

和文冊子 ● N Mori, M Oka, S Hazama, N Iizuka, K Yamamoto, S Yoshino, Akira Tangoku, Takafumi Noma and K Hirose : Detection of telomerase activity in peritoneal lavage fluid from patients with gastric cancer using immunomagnetic beads, British Journal of Cancer, Vol.83, No.8, 1026-1032, 2000.
欧文冊子 ● N Mori, M Oka, S Hazama, N Iizuka, K Yamamoto, S Yoshino, Akira Tangoku, Takafumi Noma and K Hirose : Detection of telomerase activity in peritoneal lavage fluid from patients with gastric cancer using immunomagnetic beads, British Journal of Cancer, Vol.83, No.8, 1026-1032, 2000.

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