『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=135683EID:135683, Map:0, LastModified:2012年8月31日(金) 18:19:31, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[野間 隆文], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Takeda Shun-ichi (日) (読)
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2. (英) Naito Takayuki (日) (読)
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[継承]
3. (英) Hama Kazuaki (日) (読)
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4.野間 隆文 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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学籍番号 (推奨):
[継承]
5. (英) Honjo Tasuku (日) (読)
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[継承]
題名 (必須): (英) Construction of chimaeric processed immunoglobulin genes containing mouse variable and human constant region sequences  (日)    [継承]
副題 (任意):
要約 (任意): (英) The specificity of monoclonal antibodies provides a powerful diagnostic and therapeutic tool in investigating human neoplasia. Radiological scanning and immunotherapy with mouse tumour-specific monoclonal antibodies have been applied to patients with some success, but a major problem is the neutralization of the mouse antibody induced by repeated administration of heterologous antibodies. To avoid or reduce such immune reactions, chimaeric immunoglobulins consisting of mouse variable (V) and human constant (C) regions can be synthesized. We have constructed a recombinant retrovirus DNA carrying genomic heavy-chain (H) variable-diversity joining (VH-D-JH) and C gamma 1 genes from different species and show here that the chimaeric intervening sequences are spliced out precisely. This procedure provides a useful method to construct the chimaeric mouse-human immunoglobulin gene to be expressed in Escherichia coli, yeast and animal cells. Unexpectedly, a hidden splice donor site in the 5'-flanking region of a human VH gene is used in place of the donor site of the leader sequence exon, resulting in the formation of the V region without the leader sequence.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) Chimera (日) (読) [継承]
4. (英) DNA, Recombinant (日) (読) [継承]
5. (英) Genes (日) (読) [継承]
6. (英) Genetic Engineering (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Immunoglobulin Constant Regions (日) (読) [継承]
9. (英) Immunoglobulin Heavy Chains (日) (読) [継承]
10. (英) Immunoglobulin Variable Region (日) (読) [継承]
11. (英) Immunoglobulin kappa-Chains (日) (読) [継承]
12. (英) Immunoglobulins (日) (読) [継承]
13. (英) Mice (日) (読) [継承]
14. (英) Plasmids (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Nature ([Nature Publishing Group])
(pISSN: 0028-0836, eISSN: 1476-4687)

ISSN (任意): 0028-0836
ISSN: 0028-0836 (pISSN: 0028-0836, eISSN: 1476-4687)
Title: Nature
Title(ISO): Nature
Publisher: Nature Publishing Group
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 314 [継承]
(必須): 6010 [継承]
(必須): 452 454 [継承]
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年月日 (必須): 西暦 1985年 4月 初日 (昭和 60年 4月 初日) [継承]
URL (任意):
DOI (任意): 10.1038/314452a0    (→Scopusで検索) [継承]
PMID (任意): 3920533    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) MedlineDate: 1985 Apr 4-10  (日)    [継承]

標準的な表示

和文冊子 ● Shun-ichi Takeda, Takayuki Naito, Kazuaki Hama, Takafumi Noma and Tasuku Honjo : Construction of chimaeric processed immunoglobulin genes containing mouse variable and human constant region sequences, Nature, Vol.314, No.6010, 452-454, 1985.
欧文冊子 ● Shun-ichi Takeda, Takayuki Naito, Kazuaki Hama, Takafumi Noma and Tasuku Honjo : Construction of chimaeric processed immunoglobulin genes containing mouse variable and human constant region sequences, Nature, Vol.314, No.6010, 452-454, 1985.

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