| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○著者 (必須): | 1. | 湯本 浩通 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
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| 2. | 中江 英明
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| 3. | 藤中 恵子
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| 4. | (英) Ebisu Shigeyuki (日) (読)
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| 5. | 松尾 敬志
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| ○題名 (必須): | □ | (英) Interleukin-6 (IL-6) and IL-8 are induced in human oral epithelial cells in response to exposure to periodontopathic Eikenella corrodens (日)
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| ○要約 (任意): | □ | (英) Periodontitis is the inflammatory response in periodontal tissues elicited by bacterial colonization in periodontal pockets. In this response, pocket epithelial cells are the first cells to come into contact with bacteria. To elucidate this mechanism, we determined the adherence of the periodontopathic bacterium Eikenella corrodens 1073, which has a GalNAc-sensitive lectin-like adhesin (EcLS), to a human oral epithelial carcinoma cell line (KB) and the induction of proinflammatory cytokine production in the cells following exposure to this bacterium in vitro. In the adherence assay, EcLS played a role as the adhesin of this bacterium in adherence to KB cells. In a reverse transcriptase PCR, significant interleukin-8 (IL-8) and IL-6 mRNA levels were induced in response to exposure to this bacterium. In an enzyme-linked immunosorbent assay after an 8-h bacterial exposure, the IL-8 and IL-6 protein levels were 13.5- and 8.3-fold higher than those in the nonexposed controls, respectively. These protein responses were time dependent. Interestingly, when E. corrodens was separated from KB cells by cell culture inserts, a slight stimulation of the IL-6 and IL-8 mRNA and secreted protein levels was seen. These results imply that the direct contact of E. corrodens 1073 with oral epithelial cells is not necessarily required for the stimulation of IL-6 and IL-8 secretion. We suggest that E. corrodens induces the epithelial cells to secrete proinflammatory cytokines which serve as an early signaling system to host immune and inflammatory cells in underlying connective tissues. (日)
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| ○キーワード (推奨): | 1. | (英) Adhesins, Bacterial (日) (読)
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| 2. | (英) Bacterial Adhesion (日) (読)
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| 3. | (英) Eikenella corrodens (日) (読)
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| 4. | (英) Epithelial Cells (日) (読)
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| 5. | (英) Gram-Negative Bacterial Infections (日) (読)
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| 6. | (英) Humans (日) (読)
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| 7. | (英) Interleukin-6 (日) (読)
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| 8. | (英) Interleukin-8 (日) (読)
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| 9. | (英) KB Cells (日) (読)
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| 10. | (英) Kinetics (日) (読)
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| 11. | (英) Lectins (日) (読)
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| 12. | (英) Mouth Mucosa (日) (読)
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| 13. | (英) Periodontitis (日) (読)
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| 14. | (英) RNA, Messenger (日) (読)
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| 15. | (英) Time Factors (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Infection and Immunity ([アメリカ微生物学会])
(pISSN: 0019-9567, eISSN: 1098-5522)
| ○ISSN (任意): | □ | 0019-9567
ISSN: 0019-9567
(pISSN: 0019-9567, eISSN: 1098-5522) Title: Infection and immunityTitle(ISO): Infect ImmunPublisher: American Society for Microbiology (NLM Catalog)
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| ○巻 (必須): | □ | 67
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 384 394
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| ○年月日 (必須): | □ | 西暦 1999年 1月 初日 (平成 11年 1月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1128/iai.67.1.384-394.1999 (→Scopusで検索)
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| ○PMID (任意): | □ | 9864240 (→Scopusで検索)
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| ○CRID (任意): |
| ○Scopus (任意): | 1. | 2-s2.0-0032952806
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| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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