『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=134550EID:134550, Map:0, LastModified:2012年9月14日(金) 16:45:14, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[副研究部長]/[大学院ヘルスバイオサイエンス研究部]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1.新家 利一
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2. (英) Naroda Takushi (日) (読)
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3. (英) Tamura Takamichi (日) (読)
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4. (英) Sasahara Kenji (日) (読)
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5.中堀 豊
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題名 (必須): (英) A rapid and simple method for sex identification by heteroduplex analysis, using denaturing high-performance liquid chromatography (DHPLC)  (日)    [継承]
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要約 (任意): (英) A novel method for sex identification, using a denaturing high-performance liquid chromatography (DHPLC) system, is described. Among many methods for identifying sex, the most popular and credible system has been the polymerase chain reaction (PCR) method, using nucleotide primer sets of the amelogenin gene, which is shared on both the X and Y chromosomes. With this conventional method, the judgment depends on detection of the size difference between the PCR products derived from the X and Y chromosomes. In this study, we adopted DHPLC to detect the difference by checking heteroduplex formation between the products, which enabled us to shorten the PCR products to 45bp and the separation time to within a period of 8min per sample. This new system may have wide applications in many different fields, such as forensic medicine, prenatal diagnosis, inbreeding of animals, and anthropology.  (日)    [継承]
キーワード (推奨): 1. (英) Sex Identification (日) (読) [継承]
2. (英) Amelogenin (日) (読) [継承]
3. (英) DHPLC (日) (読) [継承]
4. (英) X chromosome (日) (読) [継承]
5.Y染色体 (Y chromosome) [継承]
発行所 (推奨): (英) Japan Society Of Human Genetics and Springer-Verlag (日) (読) [継承]
誌名 (必須): Journal of Human Genetics (日本人類遺伝学会)
(pISSN: 1434-5161, eISSN: 1435-232X)

ISSN (任意): 1434-5161
ISSN: 1434-5161 (pISSN: 1434-5161, eISSN: 1435-232X)
Title: Journal of human genetics
Title(ISO): J Hum Genet
Publisher: Springer Science+Business Media
 (NLM Catalog  (医中誌Web  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 46 [継承]
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年月日 (必須): 西暦 2001年 0月 初日 (平成 13年 0月 初日) [継承]
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DOI (任意): 10.1007/s100380170076    (→Scopusで検索) [継承]
PMID (任意): 11355016    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Public Health, School of Medicine, University of Tokushima, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Toshikatsu Shinka, Takushi Naroda, Takamichi Tamura, Kenji Sasahara and Yutaka Nakahori : A rapid and simple method for sex identification by heteroduplex analysis, using denaturing high-performance liquid chromatography (DHPLC), Journal of Human Genetics, Vol.46, No.5, 263-266, 2001.
欧文冊子 ● Toshikatsu Shinka, Takushi Naroda, Takamichi Tamura, Kenji Sasahara and Yutaka Nakahori : A rapid and simple method for sex identification by heteroduplex analysis, using denaturing high-performance liquid chromatography (DHPLC), Journal of Human Genetics, Vol.46, No.5, 263-266, 2001.

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