『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=134542EID:134542, Map:0, LastModified:2013年6月28日(金) 11:32:05, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[副研究部長]/[大学院ヘルスバイオサイエンス研究部]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1. (英) Jabasini Mohammad (日) (読)
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2. (英) Zhang Lihua (日) (読)
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3. (英) Dang Fuguan (日) (読)
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4. (英) Xu Feng (日) (読)
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5. (英) Almofti R Mohamad (日) (読)
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6. (英) Ewis A Ashraf (日) (読)
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7. (英) Lee Juwon (日) (読)
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8.中堀 豊
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9. (英) Baba Yoshinobu (日) (読)
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題名 (必須): (英) Analysis of DNA polymorphisms on the human Y-chromosome by microchip electrophoresis  (日)    [継承]
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要約 (任意): (英) Validation of microchip electrophoresis in DNA analysis has been carried out using an Agilent 2100 Bioanalyzer. With a DNA 500 Assay Kit, the reproducibility and accuracy of fragment sizing of a 10 bp DNA ladder have been shown to be satisfactory with the relative standard deviation and the relative error mostly below 1.0 and 5.0% (n = 12), respectively. Both intraday and interday validations of fragment sizing and quantitation have also been performed with a 7500 Assay Kit (n = 48). Although the results of quantitation are not as good as that of sizing, due to the manual introduction of samples and markers into the chip wells, they are still sufficient to carry out further analyses of practical samples. Based on such reliable results, fast analysis of DNA polymorphisms on the human Y-chromosome has been realized with microchip electrophoresis. The total analysis times of three genomic polymorphisms on the Y-chromosome, Y Alu polymorphism, 47z/StuI, and 12f2, are all within 100 s, and the relative standard deviation and relative error of fragment sizes are below 3.5 and 3.7%, respectively. In addition, a mixture of nine DNA markers on the human Y-chromosome related to examine the cause of spermatogenic failure have been separated successfully with the smallest fragment size difference of 7 bp. Our results demonstrate the potential of microchip electrophoresis in polymorphism analysis with the advantages of high speed, good reproducibility, high precision, and high resolution.  (日)    [継承]
キーワード (推奨): 1. (英) DNA polymorphism (日) (読) [継承]
2. (英) Genomic polymorphism (日) (読) [継承]
3. (英) Microchip electrophoresis (日) (読) [継承]
4. (英) Quantitation accuracy (日) (読) [継承]
5. (英) Sizing accuracy (日) (読) [継承]
発行所 (推奨): (英) WILEY-VCH Verlag GmbH (日) (読) [継承]
誌名 (必須): Electrophoresis (Electrophoresis Society)
(pISSN: 0173-0835, eISSN: 1522-2683)

ISSN (任意): 0173-0835
ISSN: 0173-0835 (pISSN: 0173-0835, eISSN: 1522-2683)
Title: Electrophoresis
Title(ISO): Electrophoresis
Publisher: Wiley
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 23 [継承]
(必須): 10 [継承]
(必須): 1537 1542 [継承]
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年月日 (必須): 西暦 2002年 5月 23日 (平成 14年 5月 23日) [継承]
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DOI (任意): 10.1002/1522-2683(200205)23:10<1537::AID-ELPS1537>3.0.CO;2-E    (→Scopusで検索) [継承]
PMID (任意): 12116166    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation, Shomachi, Tokushima, Japan. c400141005@stud.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Mohammad Jabasini, Lihua Zhang, Fuguan Dang, Feng Xu, Mohamad R Almofti, Ashraf A Ewis, Juwon Lee, Yutaka Nakahori and Yoshinobu Baba : Analysis of DNA polymorphisms on the human Y-chromosome by microchip electrophoresis, Electrophoresis, Vol.23, No.10, 1537-1542, 2002.
欧文冊子 ● Mohammad Jabasini, Lihua Zhang, Fuguan Dang, Feng Xu, Mohamad R Almofti, Ashraf A Ewis, Juwon Lee, Yutaka Nakahori and Yoshinobu Baba : Analysis of DNA polymorphisms on the human Y-chromosome by microchip electrophoresis, Electrophoresis, Vol.23, No.10, 1537-1542, 2002.

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