| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Asahara Takashi (日) (読) あさはら たかし
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| 2. | 四宮 加容 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.眼科学])
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| 3. | 内藤 毅
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| 4. | 塩田 洋
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| ○題名 (必須): | □ | (英) Induction of Gene into the Rabbit Eye by Iontophoresis:Preliminary Report (日)
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| ○要約 (任意): | □ | (英) To assess the transfer of 6-carboxyfluorescein (6-FAM)-labeled phosphorothioate oligonucleotides(S-ODNs) into the ocular tissues, their stability, and possibility of injury to the ocular tissues. The S-ODNs(2 mL/eye)were transduced noninvasively into albino rabbit eyes.The iontophoresis group consisted of 6 rabbits (12 eyes); the control group consisted of 2 rabbits (4 eyes) given eye drops containing S-ODNs. Aqueous humor and vitreous humor were collected after iontophoresis, subjected to electrophoresis with a fluorescence DNA sequencer and analyzed by the Gene Scan program. Frozen sections, 10-microm thick, were prepared for observation under a fluorescence microscope. A plasmid 4.7 kbp in size that expresses green fluorescent protein (GFP) was induced into the 18 eyes of 9 rabbits by the same procedure. In the iontophoresis group, S-ODNs were detected in the anterior chamber 5 minutes after electrophoresis began and in the vitreous after 10 minutes. These S-ODNs maintained the same length as at the initial synthesis. The S-ODNs could also be detected in the posterior retina 20 minutes after electrophoresis. No evidence of degeneration or inflammation due to the above procedure was found in the ocular tissues. Fluorescence showing GFP gene expression was found in the cornea, the anterior chamber angle, and the ciliary subepithelial tissues. These findings show that iontophoresis is an effective method to induce genes into the rabbit eye. (日)
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| ○キーワード (推奨): | 1. | (英) Gene transduction (日) (読)
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| 2. | (英) green fluorescent protein (日) (読)
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| 3. | (英) iontophoresis (日) (読)
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| 4. | (英) phosphorothioatc oligonucleotide (日) (読)
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| ○発行所 (推奨): | □ | Elsevier Science
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| ○誌名 (必須): | □ | Japanese Journal of Ophthalmology ([日本眼科学会])
(pISSN: 0021-5155, eISSN: 1613-2246)
| ○ISSN (任意): | □ | 0021-5155
ISSN: 0021-5155
(pISSN: 0021-5155, eISSN: 1613-2246) Title: Japanese journal of ophthalmologyTitle(ISO): Jpn J OphthalmolPublisher: Springer (NLM Catalog)
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| ○巻 (必須): | □ | 45
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 31 39
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2001年 1月 初日 (平成 13年 1月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/S0021-5155(00)00291-4 (→Scopusで検索)
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| ○PMID (任意): | □ | 11163043 (→Scopusで検索)
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| ○Scopus (任意): | □ | 2-s2.0-0035191885
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| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) MedlineDate: 2001 Jan-Feb (日)
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