『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=132634EID:132634, Map:0, LastModified:2012年10月24日(水) 16:05:05, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[細井 和雄], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座 (2004年4月1日〜2015年3月31日) [継承]
著者 (必須): 1.姚 陳娟 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])
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2. (英) Karabasil, Ratko, Mileva (日) (読) カラバシル ラトコ ミレーバ
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3. (英) Purwanti, Nunuk (日) (読) プルワンティ ヌヌク
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4.李 雪飛
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5.赤松 徹也 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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6.金森 憲雄
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7.細井 和雄
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題名 (必須): (英) Tissue kallikrein mK13 is a candidate processing enzyme for the precursor of interleukin-1b in the submandibular gland of mice  (日)    [継承]
副題 (任意):
要約 (任意): (英) By using Western blot analysis, high levels of 17.5- and 20-kDa interleukin-1beta (IL-1beta) proteins were detected in the submandibular gland (SMG) of mice. Despite this fact, the amount of pro-IL-1beta protein, a precursor of IL-1beta, with a molecular size of 35 kDa in this tissue was below the detectable level, although strong expression of pro-IL-1beta mRNA was observed. A large amount of 17.5-kDa IL-1beta also appeared in the saliva of mice injected with lipopolysaccharide, suggesting that this IL-1beta is a secretory form produced by the SMG. The protein for IL-1beta-converting enzyme, a processing enzyme for pro-IL-1beta, was expressed only at a low level in the SMG as compared with its level in various epithelial tissues or lipopolysaccharide-stimulated macrophages. On the other hand, mK1, mK9, mK13, and mK22, members of the kallikrein family, were detected strongly in the SMG but not in other tissues. By incubation with mK13, but not with mK1, mK9, or mK22, the 35-kDa pro-IL-1beta was cleaved into two major products with molecular masses of 17.5 and 22 kDa, and production was inhibited by phenylmethylsulfonyl fluoride, a serine protease inhibitor, but not by IL-1beta-converting enzyme inhibitors. A peptide segment corresponding to amino acid residues 107-121 of mouse pro-IL-1beta (107WDDDDNLLVCDVPIR) was cleaved by incubation with mK13, generating two peptides, 107WDDDDNL and 114LVCDVPIR. Therefore, kallikrein mK13 would appear to hydrolyze pro-IL-1beta between its Leu113 and Leu114 residues. The results of immunohistochemistry and an autonomic therapy experiment showed that IL-1beta and kallikrein mK13 were co-localized in the secretory granules of granular convoluted tubular cells. Our present results thus suggest kallikrein mK13 is a plausible candidate for the processing enzyme for pro-IL-1beta in the SMG of mice.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Blotting, Western (日) (読) [継承]
3. (英) Caspases (日) (読) [継承]
4. (英) Electrophoresis (日) (読) [継承]
5. (英) Enzyme Inhibitors (日) (読) [継承]
6. (英) Enzyme-Linked Immunosorbent Assay (日) (読) [継承]
7. (英) Epithelium (日) (読) [継承]
8. (英) Hydrolysis (日) (読) [継承]
9. (英) Immunoblotting (日) (読) [継承]
10. (英) Immunohistochemistry (日) (読) [継承]
11. (英) Interleukin-1 (日) (読) [継承]
12. (英) Isoenzymes (日) (読) [継承]
13. (英) Kallikreins (日) (読) [継承]
14. (英) Leucine (日) (読) [継承]
15. (英) Lipopolysaccharides (日) (読) [継承]
16. (英) Macrophages (日) (読) [継承]
17. (英) Mice (日) (読) [継承]
18. (英) Mice, Inbred C3H (日) (読) [継承]
19. (英) Models, Genetic (日) (読) [継承]
20. (英) Peptides (日) (読) [継承]
21. (英) Phenylmethylsulfonyl Fluoride (日) (読) [継承]
22. (英) Protease Inhibitors (日) (読) [継承]
23. (英) Protein Binding (日) (読) [継承]
24. (英) RNA, Messenger (日) (読) [継承]
25. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
26. (英) Saliva (日) (読) [継承]
27. (英) Serine Endopeptidases (日) (読) [継承]
28. (英) Submandibular Gland (日) (読) [継承]
29. (英) Time Factors (日) (読) [継承]
30. (英) Tissue Kallikreins (日) (読) [継承]
発行所 (推奨): The American Society for Biochemistry and Molecular Biology [継承]
誌名 (必須): The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)

ISSN (任意): 0021-9258
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 7968 7976 [継承]
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年月日 (必須): 西暦 2006年 3月 初日 (平成 18年 3月 初日) [継承]
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DOI (任意): 10.1074/jbc.M507705200    (→Scopusで検索) [継承]
PMID (任意): 16423834    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Molecular Oral Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School, 3-18-15, Kuramoto-Cho, Tokushima-Shi, Tokushima 770-8504, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Chenjuan Yao, Mileva Ratko Karabasil, Nunuk Purwanti, Xuefei Li, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi : Tissue kallikrein mK13 is a candidate processing enzyme for the precursor of interleukin-1b in the submandibular gland of mice, The Journal of Biological Chemistry, Vol.281, No.12, 7968-7976, 2006.
欧文冊子 ● Chenjuan Yao, Mileva Ratko Karabasil, Nunuk Purwanti, Xuefei Li, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi : Tissue kallikrein mK13 is a candidate processing enzyme for the precursor of interleukin-1b in the submandibular gland of mice, The Journal of Biological Chemistry, Vol.281, No.12, 7968-7976, 2006.

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