『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=127936EID:127936, Map:0, LastModified:2014年4月11日(金) 11:02:47, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[片岡 佳子], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1.桑原 知巳
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2. (英) Norimatsu Izumi (日) (読)
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3.中山 治之
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4. (英) Akimoto Shigeru (日) (読)
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5.片岡 佳子 ([徳島大学.大学院医歯薬学研究部.保健学域.保健科学部門.医用検査学系.微生物・遺伝子解析学])
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6.有持 秀喜 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])
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7.大西 克成
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題名 (必須): (英) Genetic variation in 16S-23S rDNA internal transcribed spacer regions and the possible use of this genetic variation for molecular diagnosis of Bacteroides species  (日)    [継承]
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要約 (任意): (英) The structural variation in 16S-23S rDNA internal transcribed spacer regions (ITS) among Bacteroides species was assessed by PCR amplification and sequencing analysis, and its possible use for molecular diagnosis of these species was evaluated. Ninety strains of the genus Bacteroides, including the species B. distasonis, B. eggerthii, B. fragilis, B. ovatus, B. thetaiotaomicron, B. uniformis and B. vulgatus, produced one to three ITS amplification products with sizes ranging from 615 to 810 bp. Some Bacteroides strains could be differentiated at species level on the basis of ITS amplification patterns and restriction fragment length polymorphism (RFLP) analysis using a four-nucleotide-recognizing enzyme, Msp I. The results of sequence analysis of ITS amplification products revealed genes for Ile-tRNA and Ala-tRNA in all strains tested. The nucleotide sequence, except for that in tRNA-coding regions, was highly variable and characteristic for each species, but a common sequence among B. fragilis, B. thetaiotaomicron and B. ovatus was observed. A digoxigenin-labeled oligonucleotide probe (named FOT1), which was designed from this conserved sequence, specifically hybridized to the ITS amplification products from B. fragilis, B. thetaiotaomicron and B. ovatus. These results suggest that the ITS region is a useful target for the development of rapid and accurate techniques for identification of Bacteroides species.  (日)    [継承]
キーワード (推奨): 1. (英) Bacteroides (日) (読) [継承]
2. (英) 16S-23S rDNA spacer (日) (読) [継承]
3. (英) Molecular diagnosis (日) (読) [継承]
発行所 (推奨): (英) (日) 日本細菌学会,日本ウィルス学会,日本免疫学会 (読) にほんさいきんがっかい にほんうぃるすがっかい にほんめんえきがっかい [継承]
誌名 (必須): Microbiology and Immunology ([日本細菌学会])
(pISSN: 0385-5600, eISSN: 1348-0421)

ISSN (任意): 0385-5600
ISSN: 0385-5600 (pISSN: 0385-5600, eISSN: 1348-0421)
Title: Microbiology and immunology
Title(ISO): Microbiol Immunol
Supplier: 財団法人 学会誌刊行センター
Publisher: Wiley Publishing
 (NLM Catalog  (医中誌Web  (J-STAGE  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2001年 0月 初日 (平成 13年 0月 初日) [継承]
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PMID (任意): 11345528    (→Scopusで検索) [継承]
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WOS (任意): 000167322800001 [継承]
Scopus (任意): 2-s2.0-0035066982 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Comparative Study  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Tomomi Kuwahara, Izumi Norimatsu, Haruyuki Nakayama, Shigeru Akimoto, Keiko Kataoka, Hideki Arimochi and Yoshinari Ohnishi : Genetic variation in 16S-23S rDNA internal transcribed spacer regions and the possible use of this genetic variation for molecular diagnosis of Bacteroides species, Microbiology and Immunology, Vol.45, No.3, 191-199, 2001.
欧文冊子 ● Tomomi Kuwahara, Izumi Norimatsu, Haruyuki Nakayama, Shigeru Akimoto, Keiko Kataoka, Hideki Arimochi and Yoshinari Ohnishi : Genetic variation in 16S-23S rDNA internal transcribed spacer regions and the possible use of this genetic variation for molecular diagnosis of Bacteroides species, Microbiology and Immunology, Vol.45, No.3, 191-199, 2001.

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