『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=126709EID:126709, Map:0, LastModified:2012年11月3日(土) 17:44:15, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[湯浅 恵造], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1.湯浅 恵造 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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2. (英) Mi-ichi Fumika (日) (読)
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3. (英) Kobayashi Tamaki (日) (読)
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4. (英) Yamanouchi Masaya (日) (読)
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5. (英) Kotera Jun (日) (読)
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6. (英) Kita Kiyoshi (日) (読)
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7. (英) Omori Kenji (日) (読)
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題名 (必須): (英) PfPDE1, a novel cGMP-specific phosphodiesterase from the human malaria parasite Plasmodium falciparum  (日)    [継承]
副題 (任意):
要約 (任意): (英) This is the first report of molecular characterization of a novel cyclic nucleotide PDE (phosphodiesterase), isolated from the human malaria parasite Plasmodium falciparum and designated PfPDE1. PfPDE1 cDNA encodes an 884-amino-acid protein, including six putative transmembrane domains in the N-terminus followed by a catalytic domain. The PfPDE1 gene is a single-copy gene consisting of two exons and a 170 bp intron. PfPDE1 transcripts were abundant in the ring form of the asexual blood stages of the parasite. The C-terminal catalytic domain of PfPDE1, produced in Escherichia coli, specifically hydrolysed cGMP with a K(m) value of 0.65 microM. Among the PDE inhibitors tested, a PDE5 inhibitor, zaprinast, was the most effective, having an IC50 value of 3.8 microM. The non-specific PDE inhibitors IBMX (3-isobutyl-1-methylxanthine), theophylline and the antimalarial chloroquine had IC50 values of over 100 microM. Membrane fractions prepared from P. falciparum at mixed asexual blood stages showed potent cGMP hydrolytic activity compared with cytosolic fractions. This hydrolytic activity was sensitive to zaprinast with an IC50 value of 4.1 microM, but insensitive to IBMX and theophylline. Furthermore, an in vitro antimalarial activity assay demonstrated that zaprinast inhibited the growth of the asexual blood parasites, with an ED50 value of 35 microM. The impact of cyclic nucleotide signalling on the cellular development of this parasite has previously been discussed. Thus this enzyme is suggested to be a novel potential target for the treatment of the disease malaria.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Cloning, Molecular (日) (読) [継承]
3. (英) Cyclic GMP (日) (読) [継承]
4. (英) Erythrocytes (日) (読) [継承]
5. (英) Gene Expression Regulation, Developmental (日) (読) [継承]
6. (英) Gene Expression Regulation, Enzymologic (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Molecular Sequence Data (日) (読) [継承]
9. (英) Phosphoric Diester Hydrolases (日) (読) [継承]
10. (英) Phylogeny (日) (読) [継承]
11. (英) Plasmodium falciparum (日) (読) [継承]
12. (英) RNA, Messenger (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 1470-8728
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 392 [継承]
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(必須): 221 229 [継承]
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年月日 (必須): 西暦 2005年 11月 15日 (平成 17年 11月 15日) [継承]
URL (任意):
DOI (任意): 10.1042/BJ20050425    (→Scopusで検索) [継承]
PMID (任意): 16038615    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Discovery Research Laboratories, Tanabe Seiyaku Co. Ltd., 2-50, Kawagishi 2-chome, Toda, Saitama 335-8505, Japan.  (日)    [継承]
2.(英) Article.DataBankList.DataBank.DataBankName: GENBANK  (日)    [継承]
3.(英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB100091  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
5.(英) OtherID: PMC1317681  (日)    [継承]

標準的な表示

和文冊子 ● Keizo Yuasa, Fumika Mi-ichi, Tamaki Kobayashi, Masaya Yamanouchi, Jun Kotera, Kiyoshi Kita and Kenji Omori : PfPDE1, a novel cGMP-specific phosphodiesterase from the human malaria parasite Plasmodium falciparum, The Biochemical Journal, Vol.392, No.1, 221-229, 2005.
欧文冊子 ● Keizo Yuasa, Fumika Mi-ichi, Tamaki Kobayashi, Masaya Yamanouchi, Jun Kotera, Kiyoshi Kita and Kenji Omori : PfPDE1, a novel cGMP-specific phosphodiesterase from the human malaria parasite Plasmodium falciparum, The Biochemical Journal, Vol.392, No.1, 221-229, 2005.

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